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Recombinant Protein to Mus musculus (Mouse) Leptin (LEP)

OB; OBS; Obesity Homolog; Obesity Factor; Obese Protein

Description Leptin (LEP)
Catalog Number W626Mu02
Organism Species Mus musculus (Mouse)
Source Prokaryotic expression
Expression System E.coli
Endotoxin Level <1.0EU per 1µg (determined by the LAL method)
Subcellular Localization n/a
Predicted Molecular Mass n/a
Accurate Molecular Mass n/a
Residues & Tags N-terminal His Tag
Buffer Formulation 20mM Tris, 150mM NaCl,pH8.0,5% Trehalose and Proclin300.
Form Supplied Lyophilized powder
Purity Purity was assessed by SDS-PAGE (≥98%) and by HPLC.
Isoelectric Point n/a
Applications Positive Control; Immunogen; SDS-PAGE; WB.
Storage Instruction We recommend a quick spin followed by reconstitution in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.
Stability The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Notes For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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