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Recombinant Protein to Canis familiaris; Canine (Dog) Epidermal Growth Factor (EGF)

URG; Beta-Urogastrone; Pro-epidermal growth factor

Description Epidermal Growth Factor (EGF)
Catalog Number W250Ca01
Organism Species Canis familiaris; Canine (Dog)
Source Prokaryotic expression
Expression System E.coli
Endotoxin Level <1.0EU per 1µg (determined by the LAL method)
Subcellular Localization Membrane; Single-pass type I membrane prote
Predicted Molecular Mass 14.4kDa
Accurate Molecular Mass n/a
Residues & Tags His367~Phe489 (Accession # Q9BEA0) with N-terminal His Tag
Buffer Formulation 20mM Tris, 150mM NaCl,pH8.0,5% Trehalose and Proclin300.
Form Supplied Lyophilized powder
Purity Purity was assessed by SDS-PAGE (≥98%) and by HPLC.
Isoelectric Point 5.6
Applications Positive Control; Immunogen; SDS-PAGE; WB.
Storage Instruction We recommend a quick spin followed by reconstitution in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.
Stability The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Notes For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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