| Description |
Vinculin (VCL) |
| Catalog Number |
V002Hu01 |
| Organism Species |
Homo sapiens (Human) |
| Source |
Prokaryotic expression |
| Expression System |
E.coli |
| Endotoxin Level |
<1.0EU per 1µg (determined by the LAL method) |
| Subcellular Localization |
Cytoplasm. cytoskeleton. Cell junction. adherens junction. Cell membrane; Peripheral membrane protein; Cytoplasmic side. focal adhesi |
| Predicted Molecular Mass |
14.6kDa |
| Accurate Molecular Mass |
n/a |
| Residues & Tags |
Lys1020~Gln1134 (Accession # P18206) with N-terminal His Tag |
| Buffer Formulation |
20mM Tris, 150mM NaCl,pH8.0,5% Trehalose and Proclin300. |
| Form Supplied |
Lyophilized powder |
| Purity |
Purity was assessed by SDS-PAGE (≥98%) and by HPLC. |
| SDS-PAGE |
 |
| Isoelectric Point |
9.0 |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB. |
| Storage Instruction |
We recommend a quick spin followed by reconstitution in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use. |
| Stability |
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition. |
| Notes |
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. |
