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Recombinant Protein to Homo sapiens (Human) Nucleoporin 85kDa (NUP85)

Nup75; FROUNT; Nuclear pore complex protein Nup85; Pericentrin-1

Description Nucleoporin 85kDa (NUP85)
Catalog Number V703Hu01
Organism Species Homo sapiens (Human)
Source Prokaryotic expression
Expression System E.coli
Endotoxin Level <1.0EU per 1µg (determined by the LAL method)
Subcellular Localization Nucleus, nuclear pore complex. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Nucleus membrane
Predicted Molecular Mass 30.9kDa
Accurate Molecular Mass n/a
Residues & Tags Arg130~Leu368 (Accession # Q9BW27) with N-terminal His Tag
Buffer Formulation 20mM Tris, 150mM NaCl,pH8.0,5% Trehalose and Proclin300.
Form Supplied Lyophilized powder
Purity Purity was assessed by SDS-PAGE (≥98%) and by HPLC.
SDS-PAGE
Isoelectric Point 5.3
Applications Positive Control; Immunogen; SDS-PAGE; WB.
Storage Instruction We recommend a quick spin followed by reconstitution in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.
Stability The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Notes For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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