Call Now

ELISA kit for Rattus norvegicus (Rat) Osteocalcin (OC)

BGLAP; OT; BGP; Bone Gla Protein; Bone Gamma-Carboxyglutamate Protein; Gamma-carboxyglutamic acid-containing protein

Description: Osteocalcin (OC)

Organism Species: Rattus norvegicus (Rat)

Sample Type: serum, plasma, tissue homogenates, cell lysates and other biological fluids

Test Method: Double-antibody Sandwich

Assay Length: 3h

Detection Range: 15.6-1000pg/mL

Sensitivity: The minimum detectable dose of this kit is typically less than 6.3pg/mL.

 

Specifity: This assay has high sensitivity and excellent specificity for detection of Osteocalcin (OC). No significant cross-reactivity or interference between Osteocalcin (OC) and analogues was observed.

Typical Standard Curve:

 

 

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Recovery

Matrices listed below were spiked with certain level of recombinant Osteocalcin (OC) and the recovery rates were calculated by comparing the measured value to the expected amount of Osteocalcin (OC) in samples.

MatrixRecovery range (%)Average(%)
serum(n=5)80-9584
EDTA plasma(n=5)80-9487
heparin plasma(n=5)80-9687

 

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Osteocalcin (OC) were tested 24 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Osteocalcin (OC) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

 

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Osteocalcin (OC) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)94%-101%96-102%99-105%99-104%
EDTA plasma(n=5)91-100%93-101%82-91%87-94%
heparin plasma(n=5)94-103%85-94%97-97%88-102%

 

Reagents and materials provided

ReagentsQuantityReagentsQuantity
Pre-coated, ready to use 96-well strip plate1Plate sealer for 96 wells4
Standard2Standard Diluent1×20mL
Detection Reagent A1×120µLAssay Diluent A1×12mL
Detection Reagent B1×120µLAssay Diluent B1×12mL
TMB Substrate1×9mLStop Solution1×6mL
Wash Buffer (30 × concentrate)1×20mLInstruction manual1

 

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

 

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Osteocalcin (OC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Osteocalcin (OC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Osteocalcin (OC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Osteocalcin (OC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Related Products:

Catalog NO. Product Name Product Type Organism species
KEW335Bo Osteocalcin (OC) ELISA kit Bos taurus; Bovine (Cattle)
KEW335Ca Osteocalcin (OC) ELISA kit Canis familiaris; Canine (Dog)
KEW335Ga Osteocalcin (OC) ELISA kit Chicken (Gallus)
KEW335Hu Osteocalcin (OC) ELISA kit Homo sapiens (Human)
KEW335Mu Osteocalcin (OC) ELISA kit Mus musculus (Mouse)
KEW335Po Osteocalcin (OC) ELISA kit Sus scrofa; Porcine (Pig)
KEW335Ra Osteocalcin (OC) ELISA kit Rattus norvegicus (Rat)
KEW335Rb Osteocalcin (OC) ELISA kit Oryctolagus cuniculus (Rabbit)
LW335Ra71 Osteocalcin (OC) Biotin Linked-Polyclonal Antibody Rattus norvegicus (Rat)
LW335Ra81 Osteocalcin (OC) FITC Linked-Polyclonal Antibody Rattus norvegicus (Rat)
MW335Bo21 Osteocalcin (OC) Monoclonal Antibody Bos taurus; Bovine (Cattle)
MW335Ca21 Osteocalcin (OC) Monoclonal Antibody Canis familiaris; Canine (Dog)
MW335Ga21 Osteocalcin (OC) Monoclonal Antibody Chicken (Gallus)
MW335Hu22 Osteocalcin (OC) Monoclonal Antibody Homo sapiens (Human)
MW335Po21 Osteocalcin (OC) Monoclonal Antibody Sus scrofa; Porcine (Pig)
MW335Ra21 Osteocalcin (OC) Monoclonal Antibody Rattus norvegicus (Rat)
MW335Rb21 Osteocalcin (OC) Monoclonal Antibody Oryctolagus cuniculus (Rabbit)
PW335Bo01 Osteocalcin (OC) Polyclonal Antibody Bos taurus; Bovine (Cattle)
PW335Ca01 Osteocalcin (OC) Polyclonal Antibody Canis familiaris; Canine (Dog)
PW335Ga01 Osteocalcin (OC) Polyclonal Antibody Chicken (Gallus)
PW335Hu01 Osteocalcin (OC) Polyclonal Antibody Homo sapiens (Human)
PW335Hu08 Osteocalcin (OC) Polyclonal Antibody Homo sapiens (Human)
PW335Mu01 Osteocalcin (OC) Polyclonal Antibody Mus musculus (Mouse)
PW335Mu02 Osteocalcin (OC) Polyclonal Antibody Mus musculus (Mouse)
PW335Po01 Osteocalcin (OC) Polyclonal Antibody Sus scrofa; Porcine (Pig)
PW335Ra01 Osteocalcin (OC) Polyclonal Antibody Rattus norvegicus (Rat)
PW335Rb51 Osteocalcin (OC) Polyclonal Antibody Oryctolagus cuniculus (Rabbit)
W335Bo01 Osteocalcin (OC) Recombinant Protein Bos taurus; Bovine (Cattle)
W335Ca01 Osteocalcin (OC) Recombinant Protein Canis familiaris; Canine (Dog)
W335Ga01 Osteocalcin (OC) Recombinant Protein Chicken (Gallus)
W335Hu01 Osteocalcin (OC) Recombinant Protein Homo sapiens (Human)
W335Mu01 Osteocalcin (OC) Recombinant Protein Mus musculus (Mouse)
W335Mu02 Osteocalcin (OC) Recombinant Protein Mus musculus (Mouse)
W335Po01 Osteocalcin (OC) Recombinant Protein Sus scrofa; Porcine (Pig)
W335Ra01 Osteocalcin (OC) Recombinant Protein Rattus norvegicus (Rat)
W335Rb01 Osteocalcin (OC) Recombinant Protein Oryctolagus cuniculus (Rabbit)