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Ribonuclease A (RNASE1)

RNASE; Rnase-A; RNase UpI-1; RIB-1; Ribonuclease,RNase A Family,1(Pancreatic)

Ribonuclease A (RNase A) is an endonuclease that cleaves single-stranded RNA. Bovine pancreatic RNase A is one of the classic model systems of protein science.RNase A is a relatively small protein. It can be characterized as a two-layer α + β protein that is folded in half to resemble a taco, with a deep cleft for binding the RNA substrate. The first layer is composed of three alpha helices (residues 3-13, 24-34 and 50-60) from the N-terminal half of the protein. The second layer consist of three β-hairpins (residues 61-74, 79-104 and 105-124 from the C-terminal half) arranged in two β-sheets. The hairpins 61-74 and 105-124 form a four-stranded, antiparallel β-sheet that lies on helix 3 (residues 50-60). The longest β-hairpin 79-104 mates with a short β-strand (residues 42-45) to form a three-stranded, antiparallel β-sheet that lies on helix 2 .

Organism species: Homo sapiens (Human)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins W466Hu01 Recombinant Ribonuclease A (RNASE1) Positive Control; Immunogen; SDS-PAGE; WB.
NONE Native Ribonuclease A (RNASE1) Bioactivity
Antibodies MW466Hu22 Monoclonal Antibody to Ribonuclease A (RNASE1) WB; IHC; ICC; IP.
PW466Hu01 Polyclonal Antibody to Ribonuclease A (RNASE1) WB; IHC; ICC; IF.
LW466Hu71 Biotin Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) ELISA(Det);IC; IH; IH(P); WB
NONE APC Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) IHC, WB
NONE HRP Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) Simple Western; WB
NONE PE Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) Flow Cytometry
NONE FITC Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) Flow Cytometry;ICC/IF
Assay Kits KEW466Hu ELISA Kit for Ribonuclease A (RNASE1) Enzyme-linked immunosorbent assay for Antigen Detection
NONE CLIA kit for Ribonuclease A (RNASE1) CLIA assay for Antigen Detection

Organism species: Mus musculus (Mouse)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins NONE Recombinant Ribonuclease A (RNASE1) Positive Control; Immunogen; SDS-PAGE; WB.
NONE Native Ribonuclease A (RNASE1) Bioactivity
Antibodies NONE Monoclonal Antibody to Ribonuclease A (RNASE1) WB; IHC; ICC; IP.
PW466Mu01 Polyclonal Antibody to Ribonuclease A (RNASE1) WB; IHC; ICC; IF.
LW466Mu71 Biotin Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) ELISA(Det);IC; IH; IH(P); WB
NONE APC Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) IHC, WB
NONE HRP Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) Simple Western; WB
NONE PE Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) Flow Cytometry
LW466Mu81 FITC Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) Flow Cytometry;ICC/IF
Assay Kits NONE ELISA Kit for Ribonuclease A (RNASE1) Enzyme-linked immunosorbent assay for Antigen Detection
NONE CLIA kit for Ribonuclease A (RNASE1) CLIA assay for Antigen Detection

Organism species: Rattus norvegicus (Rat)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins NONE Recombinant Ribonuclease A (RNASE1) Positive Control; Immunogen; SDS-PAGE; WB.
NONE Native Ribonuclease A (RNASE1) Bioactivity
Antibodies NONE Monoclonal Antibody to Ribonuclease A (RNASE1) WB; IHC; ICC; IP.
PW466Ra01 Polyclonal Antibody to Ribonuclease A (RNASE1) WB; IHC; ICC; IF.
LW466Ra71 Biotin Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) ELISA(Det);IC; IH; IH(P); WB
NONE APC Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) IHC, WB
NONE HRP Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) Simple Western; WB
NONE PE Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) Flow Cytometry
LW466Ra81 FITC Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) Flow Cytometry;ICC/IF
Assay Kits NONE ELISA Kit for Ribonuclease A (RNASE1) Enzyme-linked immunosorbent assay for Antigen Detection
NONE CLIA kit for Ribonuclease A (RNASE1) CLIA assay for Antigen Detection

Organism species: Canis familiaris; Canine (Dog)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins NONE Recombinant Ribonuclease A (RNASE1) Positive Control; Immunogen; SDS-PAGE; WB.
NONE Native Ribonuclease A (RNASE1) Bioactivity
Antibodies NONE Monoclonal Antibody to Ribonuclease A (RNASE1) WB; IHC; ICC; IP.
NONE Polyclonal Antibody to Ribonuclease A (RNASE1) WB; IHC; ICC; IF.
NONE Biotin Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) ELISA(Det);IC; IH; IH(P); WB
NONE APC Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) IHC, WB
NONE HRP Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) Simple Western; WB
NONE PE Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) Flow Cytometry
NONE FITC Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) Flow Cytometry;ICC/IF
Assay Kits NONE ELISA Kit for Ribonuclease A (RNASE1) Enzyme-linked immunosorbent assay for Antigen Detection
NONE CLIA kit for Ribonuclease A (RNASE1) CLIA assay for Antigen Detection

Organism species: Sus scrofa; Porcine (Pig)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins NONE Recombinant Ribonuclease A (RNASE1) Positive Control; Immunogen; SDS-PAGE; WB.
NONE Native Ribonuclease A (RNASE1) Bioactivity
Antibodies NONE Monoclonal Antibody to Ribonuclease A (RNASE1) WB; IHC; ICC; IP.
NONE Polyclonal Antibody to Ribonuclease A (RNASE1) WB; IHC; ICC; IF.
NONE Biotin Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) ELISA(Det);IC; IH; IH(P); WB
NONE APC Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) IHC, WB
NONE HRP Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) Simple Western; WB
NONE PE Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) Flow Cytometry
NONE FITC Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) Flow Cytometry;ICC/IF
Assay Kits NONE ELISA Kit for Ribonuclease A (RNASE1) Enzyme-linked immunosorbent assay for Antigen Detection
NONE CLIA kit for Ribonuclease A (RNASE1) CLIA assay for Antigen Detection

Organism species: Bos taurus; Bovine (Cattle)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins NONE Recombinant Ribonuclease A (RNASE1) Positive Control; Immunogen; SDS-PAGE; WB.
NONE Native Ribonuclease A (RNASE1) Bioactivity
Antibodies MW466Bo21 Monoclonal Antibody to Ribonuclease A (RNASE1) WB; IHC; ICC; IP.
PW466Bo01 Polyclonal Antibody to Ribonuclease A (RNASE1) WB; IHC; ICC; IF.
LW466Bo71 Biotin Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) ELISA(Det);IC; IH; IH(P); WB
NONE APC Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) IHC, WB
NONE HRP Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) Simple Western; WB
NONE PE Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) Flow Cytometry
NONE FITC Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) Flow Cytometry;ICC/IF
Assay Kits KEW466Bo ELISA Kit for Ribonuclease A (RNASE1) Enzyme-linked immunosorbent assay for Antigen Detection
KCW466Bo CLIA kit for Ribonuclease A (RNASE1) CLIA assay for Antigen Detection

Organism species: Rhesus monkey (Simian)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins NONE Recombinant Ribonuclease A (RNASE1) Positive Control; Immunogen; SDS-PAGE; WB.
NONE Native Ribonuclease A (RNASE1) Bioactivity
Antibodies NONE Monoclonal Antibody to Ribonuclease A (RNASE1) WB; IHC; ICC; IP.
NONE Polyclonal Antibody to Ribonuclease A (RNASE1) WB; IHC; ICC; IF.
NONE Biotin Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) ELISA(Det);IC; IH; IH(P); WB
NONE APC Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) IHC, WB
NONE HRP Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) Simple Western; WB
NONE PE Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) Flow Cytometry
NONE FITC Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) Flow Cytometry;ICC/IF
Assay Kits NONE ELISA Kit for Ribonuclease A (RNASE1) Enzyme-linked immunosorbent assay for Antigen Detection
NONE CLIA kit for Ribonuclease A (RNASE1) CLIA assay for Antigen Detection

Organism species: Chicken (Gallus)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins NONE Recombinant Ribonuclease A (RNASE1) Positive Control; Immunogen; SDS-PAGE; WB.
NONE Native Ribonuclease A (RNASE1) Bioactivity
Antibodies NONE Monoclonal Antibody to Ribonuclease A (RNASE1) WB; IHC; ICC; IP.
NONE Polyclonal Antibody to Ribonuclease A (RNASE1) WB; IHC; ICC; IF.
NONE Biotin Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) ELISA(Det);IC; IH; IH(P); WB
NONE APC Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) IHC, WB
NONE HRP Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) Simple Western; WB
NONE PE Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) Flow Cytometry
NONE FITC Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) Flow Cytometry;ICC/IF
Assay Kits NONE ELISA Kit for Ribonuclease A (RNASE1) Enzyme-linked immunosorbent assay for Antigen Detection
NONE CLIA kit for Ribonuclease A (RNASE1) CLIA assay for Antigen Detection

Organism species: Ovis aries; Ovine (Sheep)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins NONE Recombinant Ribonuclease A (RNASE1) Positive Control; Immunogen; SDS-PAGE; WB.
NONE Native Ribonuclease A (RNASE1) Bioactivity
Antibodies NONE Monoclonal Antibody to Ribonuclease A (RNASE1) WB; IHC; ICC; IP.
NONE Polyclonal Antibody to Ribonuclease A (RNASE1) WB; IHC; ICC; IF.
NONE Biotin Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) ELISA(Det);IC; IH; IH(P); WB
NONE APC Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) IHC, WB
NONE HRP Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) Simple Western; WB
NONE PE Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) Flow Cytometry
NONE FITC Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) Flow Cytometry;ICC/IF
Assay Kits NONE ELISA Kit for Ribonuclease A (RNASE1) Enzyme-linked immunosorbent assay for Antigen Detection
NONE CLIA kit for Ribonuclease A (RNASE1) CLIA assay for Antigen Detection

Organism species: Oryctolagus cuniculus (Rabbit)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins NONE Recombinant Ribonuclease A (RNASE1) Positive Control; Immunogen; SDS-PAGE; WB.
NONE Native Ribonuclease A (RNASE1) Bioactivity
Antibodies NONE Monoclonal Antibody to Ribonuclease A (RNASE1) WB; IHC; ICC; IP.
NONE Polyclonal Antibody to Ribonuclease A (RNASE1) WB; IHC; ICC; IF.
NONE Biotin Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) ELISA(Det);IC; IH; IH(P); WB
NONE APC Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) IHC, WB
NONE HRP Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) Simple Western; WB
NONE PE Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) Flow Cytometry
NONE FITC Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) Flow Cytometry;ICC/IF
Assay Kits NONE ELISA Kit for Ribonuclease A (RNASE1) Enzyme-linked immunosorbent assay for Antigen Detection
NONE CLIA kit for Ribonuclease A (RNASE1) CLIA assay for Antigen Detection

Organism species: Cavia (Guinea pig )

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins NONE Recombinant Ribonuclease A (RNASE1) Positive Control; Immunogen; SDS-PAGE; WB.
NONE Native Ribonuclease A (RNASE1) Bioactivity
Antibodies NONE Monoclonal Antibody to Ribonuclease A (RNASE1) WB; IHC; ICC; IP.
NONE Polyclonal Antibody to Ribonuclease A (RNASE1) WB; IHC; ICC; IF.
NONE Biotin Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) ELISA(Det);IC; IH; IH(P); WB
NONE APC Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) IHC, WB
NONE HRP Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) Simple Western; WB
NONE PE Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) Flow Cytometry
NONE FITC Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) Flow Cytometry;ICC/IF
Assay Kits NONE ELISA Kit for Ribonuclease A (RNASE1) Enzyme-linked immunosorbent assay for Antigen Detection
NONE CLIA kit for Ribonuclease A (RNASE1) CLIA assay for Antigen Detection

Organism species: Equus caballus; Equine (Horse)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins NONE Recombinant Ribonuclease A (RNASE1) Positive Control; Immunogen; SDS-PAGE; WB.
NONE Native Ribonuclease A (RNASE1) Bioactivity
Antibodies NONE Monoclonal Antibody to Ribonuclease A (RNASE1) WB; IHC; ICC; IP.
NONE Polyclonal Antibody to Ribonuclease A (RNASE1) WB; IHC; ICC; IF.
NONE Biotin Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) ELISA(Det);IC; IH; IH(P); WB
NONE APC Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) IHC, WB
NONE HRP Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) Simple Western; WB
NONE PE Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) Flow Cytometry
NONE FITC Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) Flow Cytometry;ICC/IF
Assay Kits NONE ELISA Kit for Ribonuclease A (RNASE1) Enzyme-linked immunosorbent assay for Antigen Detection
NONE CLIA kit for Ribonuclease A (RNASE1) CLIA assay for Antigen Detection

Organism species: Capra hircus; Caprine (Goat)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins NONE Recombinant Ribonuclease A (RNASE1) Positive Control; Immunogen; SDS-PAGE; WB.
NONE Native Ribonuclease A (RNASE1) Bioactivity
Antibodies NONE Monoclonal Antibody to Ribonuclease A (RNASE1) WB; IHC; ICC; IP.
NONE Polyclonal Antibody to Ribonuclease A (RNASE1) WB; IHC; ICC; IF.
NONE Biotin Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) ELISA(Det);IC; IH; IH(P); WB
NONE APC Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) IHC, WB
NONE HRP Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) Simple Western; WB
NONE PE Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) Flow Cytometry
NONE FITC Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) Flow Cytometry;ICC/IF
Assay Kits NONE ELISA Kit for Ribonuclease A (RNASE1) Enzyme-linked immunosorbent assay for Antigen Detection
NONE CLIA kit for Ribonuclease A (RNASE1) CLIA assay for Antigen Detection

Organism species: Pan-species (General)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins NONE Recombinant Ribonuclease A (RNASE1) Positive Control; Immunogen; SDS-PAGE; WB.
NONE Native Ribonuclease A (RNASE1) Bioactivity
Antibodies NONE Monoclonal Antibody to Ribonuclease A (RNASE1) WB; IHC; ICC; IP.
NONE Polyclonal Antibody to Ribonuclease A (RNASE1) WB; IHC; ICC; IF.
NONE Biotin Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) ELISA(Det);IC; IH; IH(P); WB
NONE APC Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) IHC, WB
NONE HRP Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) Simple Western; WB
NONE PE Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) Flow Cytometry
NONE FITC Linked-Polyclonal Antibody to Ribonuclease A (RNASE1) Flow Cytometry;ICC/IF
Assay Kits NONE ELISA Kit for Ribonuclease A (RNASE1) Enzyme-linked immunosorbent assay for Antigen Detection
NONE CLIA kit for Ribonuclease A (RNASE1) CLIA assay for Antigen Detection

Reference

"Nucleotide sequence encoding human pancreatic ribonuclease."
Biochim. Biophys. Acta 1218:466-468(1994) [PubMed] [Europe PMC] [Abstract]

"Duplication and divergence of two distinct pancreatic ribonuclease genes in leaf-eating African and Asian colobine monkeys."
Mol. Biol. Evol. 23:1465-1479(2006) [PubMed] [Europe PMC] [Abstract]

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Primary structure of the coding part of the gene for human pancreatic ribonuclease and its chromosomal location."
Bioorg. Khim. 21:691-694(1995) [PubMed] [Europe PMC] [Abstract]

"Expression in mammalian cells, purification and characterization of recombinant human pancreatic ribonuclease."
FEBS Lett. 369:352-352(1995) [PubMed] [Europe PMC] [Abstract]

"The DNA sequences of the human and hamster secretory ribonucleases determined with the polymerase chain reaction (PCR)."
Nucleic Acids Res. 20:612-612(1992) [PubMed] [Europe PMC] [Abstract]

"The amino acid sequence of human pancreatic ribonuclease."
Anal. Biochem. 136:48-64(1984) [PubMed] [Europe PMC] [Abstract]

"Differences in glycosylation pattern of human secretory ribonucleases."
Biochem. J. 255:501-505(1988) [PubMed] [Europe PMC] [Abstract]

"Purification and characterization of three ribonucleases from human kidney: comparison with urine ribonucleases."
Arch. Biochem. Biophys. 281:144-151(1990) [PubMed] [Europe PMC] [Abstract]

"A putative mouse oocyte maturation inhibitory protein from urine of pregnant women: N-terminal sequence homology with human nonsecretory ribonuclease."
Chem. Pharm. Bull. 39:146-149(1991) [PubMed] [Europe PMC] [Abstract]

"Characterization of a unique nonsecretory ribonuclease from urine of pregnant women."
J. Biochem. 111:325-330(1992) [PubMed] [Europe PMC] [Abstract]

"Two distinct secretory ribonucleases from human cerebrum: purification, characterization and relationships to other ribonucleases."
Biochem. J. 296:617-625(1993) [PubMed] [Europe PMC] [Abstract]

"Three-dimensional structure of a human pancreatic ribonuclease variant, a step forward in the design of cytotoxic ribonucleases."
J. Mol. Biol. 303:49-60(2000) [PubMed] [Europe PMC] [Abstract]

"Three-dimensional structure of human RNase 1 delta N7 at 1.9 A resolution."
Acta Crystallogr. D 57:498-505(2001) [PubMed] [Europe PMC] [Abstract]

"The structure of an engineered domain-swapped ribonuclease dimer and its implications for the evolution of proteins toward oligomerization."
Structure 9:967-976(2001) [PubMed] [Europe PMC] [Abstract]

"Inhibition of human pancreatic ribonuclease by the human ribonuclease inhibitor protein."
J. Mol. Biol. 368:434-449(2007) [PubMed] [Europe PMC] [Abstract]

"'Crystal lattice engineering,' an approach to engineer protein crystal contacts by creating intermolecular symmetry: crystallization and structure determination of a mutant human RNase 1 with a hydrophobic interface of leucines."
Protein Sci. 16:1389-1397(2007) [PubMed] [Europe PMC] [Abstract]