Neutrophil Cytosolic Factor 2 (NCF2)

NOXA2; p67phox; 65kDa, Chronic Granulomatous Disease,Autosomal 2; NADPH Oxidase Activator 2; 67 kDa neutrophil oxidase factor; Neutrophil NADPH oxidase factor 2

SPN	W465
HGNC	7661
MGI	97284
PubMed	24163247
RGD	1309424
UniProt	P19878
Wiki	NCF2

Neutrophil cytosol factor 2 is the 67-kilodalton cytosolic subunit of the multi-protein complex known as NADPH oxidase found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in this gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease.

Using neutrophils from a patient with p67-deficient CGD, Okamura et al. (1990) confirmed that the p67 protein functions in the respiratory burst mediated by NADPH oxidase and that p67 may be complexed to p47-phox (NCF1) while it participates in oxidase activation.

Reference

"Cloning of a 67-kD neutrophil oxidase factor with similarity to a noncatalytic region of p60c-src."
Science 248:727-730(1990) [PubMed] [Europe PMC] [Abstract]

"Characterization of the p67phox gene: genomic organization and restriction fragment length polymorphism analysis for prenatal diagnosis in chronic granulomatous disease."
Blood 82:3739-3744(1993) [PubMed] [Europe PMC] [Abstract]

"Expression of a p67(phox) homolog in Caco-2 cells giving O(2)(-)-reconstituting ability to cytochrome b(558) together with recombinant p47(phox)."
Biochem. Biophys. Res. Commun. 296:1322-1328(2002) [PubMed] [Europe PMC] [Abstract]

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

"The DNA sequence and biological annotation of human chromosome 1."
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"JFC1, a novel tandem C2 domain-containing protein associated with the leukocyte NADPH oxidase."
J. Biol. Chem. 276:18855-18862(2001) [PubMed] [Europe PMC] [Abstract]

"Novel human homologues of p47phox and p67phox participate in activation of superoxide-producing NADPH oxidases."
J. Biol. Chem. 278:25234-25246(2003) [PubMed] [Europe PMC] [Abstract]

"The arachidonic acid-binding protein S100A8/A9 promotes NADPH oxidase activation by interaction with p67phox and Rac-2."
FASEB J. 19:467-469(2005) [PubMed] [Europe PMC] [Abstract]

"Full-length p40phox structure suggests a basis for regulation mechanism of its membrane binding."
EMBO J. 26:1176-1186(2007) [PubMed] [Europe PMC] [Abstract]

"Structure of the TPR domain of p67phox in complex with Rac.GTP."
Mol. Cell 6:899-907(2000) [PubMed] [Europe PMC] [Abstract]

"Diverse recognition of non-PxxP peptide ligands by the SH3 domains from p67(phox), Grb2 and Pex13p."
EMBO J. 21:4268-4276(2002) [PubMed] [Europe PMC] [Abstract]

"PB1 domain-mediated heterodimerization in NADPH oxidase and signaling complexes of atypical protein kinase C with Par6 and p62."
Mol. Cell 12:39-50(2003) [PubMed] [Europe PMC] [Abstract]

"Solution structure of the 1st SH3 domain from human neutrophil cytosol factor 2 (NCF-2)."
Submitted (MAY-2006) to the PDB data bank

"Autosomal recessive chronic granulomatous disease with absence of the 67-kD cytosolic NADPH oxidase component: identification of mutation and detection of carriers."
Blood 83:531-536(1994) [PubMed] [Europe PMC] [Abstract]

"Identification of a double mutation (D160V-K161E) (sic) in the p67phox gene of a chronic granulomatous disease patient."
Biochem. Biophys. Res. Commun. 231:861-863(1997) [PubMed] [Europe PMC] [Abstract]

"Molecular characterization of autosomal recessive chronic granulomatous disease caused by a defect of the nicotinamide adenine dinucleotide phosphate (reduced form) oxidase component p67-phox."
Blood 94:2505-2514(1999) [PubMed] [Europe PMC] [Abstract]

"Autosomal recessive chronic granulomatous disease caused by novel mutations in NCF-2, the gene encoding the p67-phox component of phagocyte NADPH oxidase."
Hum. Genet. 105:460-467(1999) [PubMed] [Europe PMC] [Abstract]

"Hematologically important mutations: the autosomal recessive forms of chronic granulomatous disease (first update)."
Blood Cells Mol. Dis. 26:561-565(2000) [PubMed] [Europe PMC] [Abstract]

"Genetic and mutational heterogeneity of autosomal recessive chronic granulomatous disease in Tunisia."
J. Hum. Genet. 51:887-895(2006) [PubMed] [Europe PMC] [Abstract]

"Focus on FOCIS: the continuing diagnostic challenge of autosomal recessive chronic granulomatous disease."
Clin. Immunol. 128:117-126(2008) [PubMed] [Europe PMC] [Abstract]

"Four different NCF2 mutations in six families from Turkey and an overview of NCF2 gene mutations."
Eur. J. Clin. Invest. 39:942-951(2009) [PubMed] [Europe PMC] [Abstract]

"Hematologically important mutations: the autosomal recessive forms of chronic granulomatous disease (second update)."
Blood Cells Mol. Dis. 44:291-299(2010) [PubMed] [Europe PMC] [Abstract]

"Clinical, functional, and genetic characterization of chronic granulomatous disease in 89 Turkish patients."
J. Allergy Clin. Immunol. 132:1156-1163(2013) [PubMed] [Europe PMC] [Abstract]