Phosphoinositide-3-Kinase Class-2-Alpha Polypeptide (PIK3C2a)

PI3K2a; CPK; PI3-K-C2(Alpha); PI3-K-C2A; PI3K2-a; Phosphoinositide 3-Kinase 2a; Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha

SPN	W420
HGNC	8971
MGI	1203729
PubMed	21963845
RGD	1310389
UniProt	O00443
Wiki	PIK3C2A

The protein encoded by this gene belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is not sensitive to nanomolar levels of the inhibitor wortmannin. This protein was shown to be able to be activated by insulin and may be involved in integrin-dependent signaling.

Reference

"Cloning of a human phosphoinositide 3-kinase with a C2 domain which displays reduced sensitivity to the inhibitor wortmannin."
Biochem. J. 326:139-147(1997) [PubMed] [Europe PMC] [Abstract]

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

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Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"The class II phosphoinositide 3-kinase PI3K-C2alpha is concentrated in the trans-Golgi network and present in clathrin-coated vesicles."
J. Biol. Chem. 275:11943-11950(2000) [PubMed] [Europe PMC] [Abstract]

"Class II phosphoinositide 3-kinases are downstream targets of activated polypeptide growth factor receptors."
Mol. Cell. Biol. 20:3817-3830(2000) [PubMed] [Europe PMC] [Abstract]

"Phosphatidylinositol 3-kinase c2alpha contains a nuclear localization sequence and associates with nuclear speckles."
J. Biol. Chem. 276:48135-48142(2001) [PubMed] [Europe PMC] [Abstract]

"The class II phosphoinositide 3-kinase C2alpha is activated by clathrin and regulates clathrin-mediated membrane trafficking."
Mol. Cell 7:443-449(2001) [PubMed] [Europe PMC] [Abstract]

"Topographical expression of class IA and class II phosphoinositide 3-kinase enzymes in normal human tissues is consistent with a role in differentiation."
BMC Clin. Pathol. 3:4-4(2003) [PubMed] [Europe PMC] [Abstract]

"Mitotic and stress-induced phosphorylation of HsPI3K-C2alpha targets the protein for degradation."
J. Biol. Chem. 278:26055-26064(2003) [PubMed] [Europe PMC] [Abstract]

"Individual phosphoinositide 3-kinase C2alpha domain activities independently regulate clathrin function."
J. Biol. Chem. 280:40766-40772(2005) [PubMed] [Europe PMC] [Abstract]

"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]

"A quantitative atlas of mitotic phosphorylation."
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]

"Large-scale proteomics analysis of the human kinome."
Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]

"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]

"PI3KC2alpha, a class II PI3K, is required for dynamin-independent internalization pathways."
J. Cell Sci. 123:4240-4250(2010) [PubMed] [Europe PMC] [Abstract]

"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]

"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

"Class II phosphoinositide 3-kinase regulates exocytosis of insulin granules in pancreatic beta cells."
J. Biol. Chem. 286:4216-4225(2011) [PubMed] [Europe PMC] [Abstract]

"Role of class II phosphoinositide 3-kinase in cell signalling."
Biochem. Soc. Trans. 35:211-214(2007) [PubMed] [Europe PMC] [Abstract]

"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]

"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]

"Structural and membrane binding analysis of the Phox homology domain of phosphoinositide 3-kinase-C2alpha."
J. Biol. Chem. 281:39396-39406(2006) [PubMed] [Europe PMC] [Abstract]

"Ligand-binding specificity of PI3kinase C2alpha PX domain."
Submitted (OCT-2006) to the PDB data bank