Protein Kinase C Iota (PKCi)
PKC-I; PRKCI; PRKC-I; NPKC-Iota; Atypical protein kinase C-lambda/iota
Protein Kinase C Iota is a protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol.
This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. There is a single exon pseudogene mapped on chromosome X.
Organism species: Homo sapiens (Human)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW312Hu01 | Recombinant Protein Kinase C Iota (PKCi) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW312Hu01 | Monoclonal Antibody to Protein Kinase C Iota (PKCi) | WB; IHC; ICC; IP. |
| PAW312Hu01 | Polyclonal Antibody to Protein Kinase C Iota (PKCi) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW312Hu01 | CLIA Kit for Protein Kinase C Iota (PKCi) | CLIA assay for Antigen Detection |
| ELW312Hu01 | ELISA Kit for Protein Kinase C Iota (PKCi) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Mus musculus (Mouse)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW312Mu01 | Recombinant Protein Kinase C Iota (PKCi) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW312Mu01 | Monoclonal Antibody to Protein Kinase C Iota (PKCi) | WB; IHC; ICC; IP. |
| PAW312Mu01 | Polyclonal Antibody to Protein Kinase C Iota (PKCi) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW312Mu01 | CLIA Kit for Protein Kinase C Iota (PKCi) | CLIA assay for Antigen Detection |
| ELW312Mu01 | ELISA Kit for Protein Kinase C Iota (PKCi) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Rattus norvegicus (Rat)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW312Ra01 | Recombinant Protein Kinase C Iota (PKCi) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW312Ra01 | Monoclonal Antibody to Protein Kinase C Iota (PKCi) | WB; IHC; ICC; IP. |
| PAW312Ra01 | Polyclonal Antibody to Protein Kinase C Iota (PKCi) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW312Ra01 | CLIA Kit for Protein Kinase C Iota (PKCi) | CLIA assay for Antigen Detection |
| ELW312Ra01 | ELISA Kit for Protein Kinase C Iota (PKCi) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Canis familiaris; Canine (Dog)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW312Ca01 | Recombinant Protein Kinase C Iota (PKCi) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW312Ca01 | Monoclonal Antibody to Protein Kinase C Iota (PKCi) | WB; IHC; ICC; IP. |
| PAW312Ca01 | Polyclonal Antibody to Protein Kinase C Iota (PKCi) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW312Ca01 | CLIA Kit for Protein Kinase C Iota (PKCi) | CLIA assay for Antigen Detection |
| ELW312Ca01 | ELISA Kit for Protein Kinase C Iota (PKCi) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Sus scrofa; Porcine (Pig)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW312Po01 | Recombinant Protein Kinase C Iota (PKCi) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW312Po01 | Monoclonal Antibody to Protein Kinase C Iota (PKCi) | WB; IHC; ICC; IP. |
| PAW312Po01 | Polyclonal Antibody to Protein Kinase C Iota (PKCi) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW312Po01 | CLIA Kit for Protein Kinase C Iota (PKCi) | CLIA assay for Antigen Detection |
| ELW312Po01 | ELISA Kit for Protein Kinase C Iota (PKCi) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Bos taurus; Bovine (Cattle)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW312Bo01 | Recombinant Protein Kinase C Iota (PKCi) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW312Bo01 | Monoclonal Antibody to Protein Kinase C Iota (PKCi) | WB; IHC; ICC; IP. |
| PAW312Bo01 | Polyclonal Antibody to Protein Kinase C Iota (PKCi) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW312Bo01 | CLIA Kit for Protein Kinase C Iota (PKCi) | CLIA assay for Antigen Detection |
| ELW312Bo01 | ELISA Kit for Protein Kinase C Iota (PKCi) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Oryctolagus cuniculus (Rabbit)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW312Rb01 | Recombinant Protein Kinase C Iota (PKCi) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW312Rb01 | Monoclonal Antibody to Protein Kinase C Iota (PKCi) | WB; IHC; ICC; IP. |
| PAW312Rb01 | Polyclonal Antibody to Protein Kinase C Iota (PKCi) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW312Rb01 | CLIA Kit for Protein Kinase C Iota (PKCi) | CLIA assay for Antigen Detection |
| ELW312Rb01 | ELISA Kit for Protein Kinase C Iota (PKCi) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Rhesus monkey (Simian)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW312Mo01 | Recombinant Protein Kinase C Iota (PKCi) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW312Mo01 | Monoclonal Antibody to Protein Kinase C Iota (PKCi) | WB; IHC; ICC; IP. |
| PAW312Mo01 | Polyclonal Antibody to Protein Kinase C Iota (PKCi) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW312Mo01 | CLIA Kit for Protein Kinase C Iota (PKCi) | CLIA assay for Antigen Detection |
| ELW312Mo01 | ELISA Kit for Protein Kinase C Iota (PKCi) | Enzyme-linked immunosorbent assay for Antigen Detection |
Reference
"Molecular cloning and characterization of PKC iota, an atypical isoform of protein kinase C derived from insulin-secreting cells."
J. Biol. Chem. 268:24296-24302(1993) [PubMed] [Europe PMC] [Abstract]
"Human protein kinase C iota gene (PRKCI) is closely linked to the BTK gene in Xq21.3."
Genomics 26:629-631(1995) [PubMed] [Europe PMC] [Abstract]
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
"Lambda-interacting protein, a novel protein that specifically interacts with the zinc finger domain of the atypical protein kinase C isotype lambda/iota and stimulates its kinase activity in vitro and in vivo."
Mol. Cell. Biol. 16:105-114(1996) [PubMed] [Europe PMC] [Abstract]
"Atypical protein kinase C iota protects human leukemia cells against drug-induced apoptosis."
J. Biol. Chem. 272:27521-27524(1997) [PubMed] [Europe PMC] [Abstract]
"Localization of atypical protein kinase C isoforms into lysosome-targeted endosomes through interaction with p62."
Mol. Cell. Biol. 18:3069-3080(1998) [PubMed] [Europe PMC] [Abstract]
"Overexpression of atypical PKC in PC12 cells enhances NGF-responsiveness and survival through an NF-kappaB dependent pathway."
Cell Death Differ. 6:753-764(1999) [PubMed] [Europe PMC] [Abstract]
"The interaction of p62 with RIP links the atypical PKCs to NF-kappaB activation."
EMBO J. 18:3044-3053(1999) [PubMed] [Europe PMC] [Abstract]
"Unique structural and functional properties of the ATP-binding domain of atypical protein kinase C-iota."
J. Biol. Chem. 275:33289-33296(2000) [PubMed] [Europe PMC] [Abstract]
"Protein kinase C iota protects neural cells against apoptosis induced by amyloid beta-peptide."
Brain Res. Mol. Brain Res. 82:107-113(2000) [PubMed] [Europe PMC] [Abstract]
"Human homologues of the Caenorhabditis elegans cell polarity protein PAR6 as an adaptor that links the small GTPases Rac and Cdc42 to atypical protein kinase C."
Genes Cells 6:107-119(2001) [PubMed] [Europe PMC] [Abstract]
"Atypical protein kinase C is involved in the evolutionarily conserved par protein complex and plays a critical role in establishing epithelia-specific junctional structures."
J. Cell Biol. 152:1183-1196(2001) [PubMed] [Europe PMC] [Abstract]
"Glyceraldehyde-3-phosphate dehydrogenase is phosphorylated by protein kinase Ciota /lambda and plays a role in microtubule dynamics in the early secretory pathway."
J. Biol. Chem. 277:3334-3341(2002) [PubMed] [Europe PMC] [Abstract]
"Nerve growth factor stimulates multisite tyrosine phosphorylation and activation of the atypical protein kinase C's via a src kinase pathway."
Mol. Cell. Biol. 21:8414-8427(2001) [PubMed] [Europe PMC] [Abstract]
"Phosphorylation of tyrosine 256 facilitates nuclear import of atypical protein kinase C."
J. Cell. Biochem. 85:42-53(2002) [PubMed] [Europe PMC] [Abstract]
"Centaurin-alpha(1) associates with and is phosphorylated by isoforms of protein kinase C."
Biochem. Biophys. Res. Commun. 307:459-465(2003) [PubMed] [Europe PMC] [Abstract]
"Mammalian Lgl forms a protein complex with PAR-6 and aPKC independently of PAR-3 to regulate epithelial cell polarity."
Curr. Biol. 13:734-743(2003) [PubMed] [Europe PMC] [Abstract]
"Atypical protein kinase C plays a critical role in protein transport from pre-Golgi intermediates."
J. Biol. Chem. 278:38015-38021(2003) [PubMed] [Europe PMC] [Abstract]
"Rab2 interacts directly with atypical protein kinase C (aPKC) iota/lambda and inhibits aPKCiota/lambda-dependent glyceraldehyde-3-phosphate dehydrogenase phosphorylation."
J. Biol. Chem. 278:52524-52530(2003) [PubMed] [Europe PMC] [Abstract]
"Regulation of interleukin receptor-associated kinase (IRAK) phosphorylation and signaling by iota protein kinase C."
J. Biol. Chem. 279:4161-4165(2004) [PubMed] [Europe PMC] [Abstract]
"Atypical protein kinase Ciota plays a critical role in human lung cancer cell growth and tumorigenicity."
J. Biol. Chem. 280:31109-31115(2005) [PubMed] [Europe PMC] [Abstract]
"Cyclin-dependent kinase activating kinase/Cdk7 co-localizes with PKC-iota in human glioma cells."
Tissue Cell 37:53-58(2005) [PubMed] [Europe PMC] [Abstract]
"Src-dependent protein kinase C iota/lambda (aPKCiota/lambda) tyrosine phosphorylation is required for aPKCiota/lambda association with Rab2 and glyceraldehyde-3-phosphate dehydrogenase on pre-Golgi intermediates."
J. Biol. Chem. 281:8436-8442(2006) [PubMed] [Europe PMC] [Abstract]
"Atypical protein kinase C (iota) activates ezrin in the apical domain of intestinal epithelial cells."
J. Cell Sci. 121:644-654(2008) [PubMed] [Europe PMC] [Abstract]
"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
"A quantitative atlas of mitotic phosphorylation."
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
"Protein kinase C iota mediates lipid-induced apoptosis of human coronary artery endothelial cells."
Microvasc. Res. 78:40-44(2009) [PubMed] [Europe PMC] [Abstract]
"Ect2 links the PKCiota-Par6alpha complex to Rac1 activation and cellular transformation."
Oncogene 28:3597-3607(2009) [PubMed] [Europe PMC] [Abstract]
"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
"PKC-? promotes glioblastoma cell survival by phosphorylating and inhibiting BAD through a phosphatidylinositol 3-kinase pathway."
Biochim. Biophys. Acta 1813:1190-1197(2011) [PubMed] [Europe PMC] [Abstract]
"Oncogenic activity of Ect2 is regulated through protein kinase C iota-mediated phosphorylation."
J. Biol. Chem. 286:8149-8157(2011) [PubMed] [Europe PMC] [Abstract]
"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
"Solution structure of atypical protein kinase C PB1 domain and its mode of interaction with ZIP/p62 and MEK5."
J. Biol. Chem. 279:31883-31890(2004) [PubMed] [Europe PMC] [Abstract]
"Structure of a cell polarity regulator, a complex between atypical PKC and Par6 PB1 domains."
J. Biol. Chem. 280:9653-9661(2005) [PubMed] [Europe PMC] [Abstract]
"Crystal structure of the catalytic domain of human atypical protein kinase C-iota reveals interaction mode of phosphorylation site in turn motif."
J. Mol. Biol. 352:918-931(2005) [PubMed] [Europe PMC] [Abstract]
"Patterns of somatic mutation in human cancer genomes."
Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]