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Myeloperoxidase (MPO)

Myeloperoxidase (MPO) is a hemoprotein that is abundantly expressed in polymorphonuclear leukocytes (neutrophils) and secreted during their activation. The presence of a peroxidase in the cytoplasmic granules of leukocytes was suggested at the beginning of 20th century but it was the early 1940s that it was purified for the first time.

Native MPO is a covalently bound tetrameric complex of two glycosylated alpha chains (MW 59 – 64 kDa) and two unglycosylated beta chains (MW 14 kDa) with total MW about 150 kDa and theoretical pI 9.2. MPO plays an important role in neutrophil microbicidal action through catalyzing chloride ion oxidation to hypochlorous acid, which is a potent antimicrobial agent.

Organism species: Homo sapiens (Human)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPW209Hu01 Recombinant Myeloperoxidase (MPO) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAW209Hu01 Monoclonal Antibody to Myeloperoxidase (MPO) WB; IHC; ICC; IP.
PAW209Hu01 Polyclonal Antibody to Myeloperoxidase (MPO) WB; IHC; ICC; IF.
Assay Kits CLW209Hu01 CLIA Kit for Myeloperoxidase (MPO) CLIA assay for Antigen Detection
ELW209Hu01 ELISA Kit for Myeloperoxidase (MPO) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Mus musculus (Mouse)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPW209Mu01 Recombinant Myeloperoxidase (MPO) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAW209Mu01 Monoclonal Antibody to Myeloperoxidase (MPO) WB; IHC; ICC; IP.
PAW209Mu01 Polyclonal Antibody to Myeloperoxidase (MPO) WB; IHC; ICC; IF.
Assay Kits CLW209Mu01 CLIA Kit for Myeloperoxidase (MPO) CLIA assay for Antigen Detection
ELW209Mu01 ELISA Kit for Myeloperoxidase (MPO) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Rattus norvegicus (Rat)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPW209Ra01 Recombinant Myeloperoxidase (MPO) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAW209Ra01 Monoclonal Antibody to Myeloperoxidase (MPO) WB; IHC; ICC; IP.
PAW209Ra01 Polyclonal Antibody to Myeloperoxidase (MPO) WB; IHC; ICC; IF.
Assay Kits CLW209Ra01 CLIA Kit for Myeloperoxidase (MPO) CLIA assay for Antigen Detection
ELW209Ra01 ELISA Kit for Myeloperoxidase (MPO) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Canis familiaris; Canine (Dog)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPW209Ca01 Recombinant Myeloperoxidase (MPO) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAW209Ca01 Monoclonal Antibody to Myeloperoxidase (MPO) WB; IHC; ICC; IP.
PAW209Ca01 Polyclonal Antibody to Myeloperoxidase (MPO) WB; IHC; ICC; IF.
Assay Kits CLW209Ca01 CLIA Kit for Myeloperoxidase (MPO) CLIA assay for Antigen Detection
ELW209Ca01 ELISA Kit for Myeloperoxidase (MPO) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Sus scrofa; Porcine (Pig)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPW209Po01 Recombinant Myeloperoxidase (MPO) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAW209Po01 Monoclonal Antibody to Myeloperoxidase (MPO) WB; IHC; ICC; IP.
PAW209Po01 Polyclonal Antibody to Myeloperoxidase (MPO) WB; IHC; ICC; IF.
Assay Kits CLW209Po01 CLIA Kit for Myeloperoxidase (MPO) CLIA assay for Antigen Detection
ELW209Po01 ELISA Kit for Myeloperoxidase (MPO) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Bos taurus; Bovine (Cattle)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPW209Bo01 Recombinant Myeloperoxidase (MPO) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAW209Bo01 Monoclonal Antibody to Myeloperoxidase (MPO) WB; IHC; ICC; IP.
PAW209Bo01 Polyclonal Antibody to Myeloperoxidase (MPO) WB; IHC; ICC; IF.
Assay Kits CLW209Bo01 CLIA Kit for Myeloperoxidase (MPO) CLIA assay for Antigen Detection
ELW209Bo01 ELISA Kit for Myeloperoxidase (MPO) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Oryctolagus cuniculus (Rabbit)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPW209Rb01 Recombinant Myeloperoxidase (MPO) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAW209Rb01 Monoclonal Antibody to Myeloperoxidase (MPO) WB; IHC; ICC; IP.
PAW209Rb01 Polyclonal Antibody to Myeloperoxidase (MPO) WB; IHC; ICC; IF.
Assay Kits CLW209Rb01 CLIA Kit for Myeloperoxidase (MPO) CLIA assay for Antigen Detection
ELW209Rb01 ELISA Kit for Myeloperoxidase (MPO) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Rhesus monkey (Simian)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPW209Mo01 Recombinant Myeloperoxidase (MPO) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAW209Mo01 Monoclonal Antibody to Myeloperoxidase (MPO) WB; IHC; ICC; IP.
PAW209Mo01 Polyclonal Antibody to Myeloperoxidase (MPO) WB; IHC; ICC; IF.
Assay Kits CLW209Mo01 CLIA Kit for Myeloperoxidase (MPO) CLIA assay for Antigen Detection
ELW209Mo01 ELISA Kit for Myeloperoxidase (MPO) Enzyme-linked immunosorbent assay for Antigen Detection

Reference

"Molecular cloning and characterization of cDNA for human myeloperoxidase."
J. Biol. Chem. 262:3844-3851(1987) [PubMed] [Europe PMC] [Abstract]

"Chromosomal gene structure of human myeloperoxidase and regulation of its expression by granulocyte colony-stimulating factor."
J. Biol. Chem. 262:15208-15213(1987) [PubMed] [Europe PMC] [Abstract]

"Isolation of a complementary DNA clone for thyroid microsomal antigen. Homology with the gene for thyroid peroxidase."
J. Clin. Invest. 80:1205-1208(1987) [PubMed] [Europe PMC] [Abstract]

"Characterization of cDNA clones for human myeloperoxidase: predicted amino acid sequence and evidence for multiple mRNA species."
Nucleic Acids Res. 15:2013-2028(1987) [PubMed] [Europe PMC] [Abstract]

"Multiple species of myeloperoxidase messenger RNAs produced by alternative splicing and differential polyadenylation."
Biochemistry 27:5906-5914(1988) [PubMed] [Europe PMC] [Abstract]

Erratum
Biochemistry 27:9226-9226(1988)

"Complete nucleotide sequence of the human myeloperoxidase gene."
Nucleic Acids Res. 17:7985-7986(1989) [PubMed] [Europe PMC] [Abstract]

"Cloning and characterization of four types of cDNA encoding myeloperoxidase from human monocytic leukemia cell line, SKM-1."
Leukemia 7:441-445(1993) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Isolation and characterization of extracellular myeloperoxidase precursor in HL-60 cell cultures."
Biochem. Biophys. Res. Commun. 166:852-859(1990) [PubMed] [Europe PMC] [Abstract]

"Glycosylation pattern of mature dimeric leukocyte and recombinant monomeric myeloperoxidase: glycosylation is required for optimal enzymatic activity."
J. Biol. Chem. 285:16351-16359(2010) [PubMed] [Europe PMC] [Abstract]

"Unique autolytic cleavage of human myeloperoxidase. Implications for the involvement of active site MET409."
J. Biol. Chem. 267:25282-25288(1992) [PubMed] [Europe PMC] [Abstract]

"Identification of transcriptional cis-elements in introns 7 and 9 of the myeloperoxidase gene."
J. Biol. Chem. 268:13479-13485(1993) [PubMed] [Europe PMC] [Abstract]

"Isolation and characterization of a cDNA coding for human myeloperoxidase."
Arch. Biochem. Biophys. 255:147-155(1987) [PubMed] [Europe PMC] [Abstract]

"Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry."
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"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

"X-ray crystal structure and characterization of halide-binding sites of human myeloperoxidase at 1.8-A resolution."
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"Structure of the green heme in myeloperoxidase."
Arch. Biochem. Biophys. 316:653-656(1995) [PubMed] [Europe PMC] [Abstract]

"Human myeloperoxidase: structure of a cyanide complex and its interaction with bromide and thiocyanate substrates at 1.9 A resolution."
Biochemistry 40:13990-13997(2001) [PubMed] [Europe PMC] [Abstract]

"Myeloperoxidase (MPO) gene mutation in hereditary MPO deficiency."
Blood 83:1935-1940(1994) [PubMed] [Europe PMC] [Abstract]

"Hereditary myeloperoxidase deficiency due to a missense mutation of arginine 569 to tryptophan."
J. Biol. Chem. 269:1212-1216(1994) [PubMed] [Europe PMC] [Abstract]

"Effect of the R569W missense mutation on the biosynthesis of myeloperoxidase."
J. Biol. Chem. 271:9546-9549(1996) [PubMed] [Europe PMC] [Abstract]

"A novel form of hereditary myeloperoxidase deficiency linked to endoplasmic reticulum/proteasome degradation."
J. Clin. Invest. 101:2900-2909(1998) [PubMed] [Europe PMC] [Abstract]

"Biochemical and molecular characterization of hereditary myeloperoxidase deficiency."
Blood 90:4126-4134(1997) [PubMed] [Europe PMC] [Abstract]

"The consensus coding sequences of human breast and colorectal cancers."
Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]