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Carbonic Anhydrase I (CA1)

CA-I; CAI; Car1; CAB; Carbonic anhydrase B; Carbonate dehydratase I

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including cellular respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid.They show extensive diversity in tissue distribution and in their subcellular localization. CA1 is closely linked to CA2 and CA3 genes on chromosome 8, and it encodes a cytosolic protein which is found at the highest level in erythrocytes. Transcript variants of CA1 utilizing alternative polyA_sites have been described in literature.CA I is the major form of the enzyme in human red cells. They found that the protein consists of 260 amino acids.

Organism species: Homo sapiens (Human)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPV936Hu01 Recombinant Carbonic Anhydrase I (CA1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAV936Hu01 Monoclonal Antibody to Carbonic Anhydrase I (CA1) WB; IHC; ICC; IP.
PAV936Hu01 Polyclonal Antibody to Carbonic Anhydrase I (CA1) WB; IHC; ICC; IF.
Assay Kits CLV936Hu01 CLIA Kit for Carbonic Anhydrase I (CA1) CLIA assay for Antigen Detection
ELV936Hu01 ELISA Kit for Carbonic Anhydrase I (CA1) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Mus musculus (Mouse)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPV936Mu01 Recombinant Carbonic Anhydrase I (CA1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAV936Mu01 Monoclonal Antibody to Carbonic Anhydrase I (CA1) WB; IHC; ICC; IP.
PAV936Mu01 Polyclonal Antibody to Carbonic Anhydrase I (CA1) WB; IHC; ICC; IF.
Assay Kits CLV936Mu01 CLIA Kit for Carbonic Anhydrase I (CA1) CLIA assay for Antigen Detection
ELV936Mu01 ELISA Kit for Carbonic Anhydrase I (CA1) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Rattus norvegicus (Rat)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPV936Ra01 Recombinant Carbonic Anhydrase I (CA1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAV936Ra01 Monoclonal Antibody to Carbonic Anhydrase I (CA1) WB; IHC; ICC; IP.
PAV936Ra01 Polyclonal Antibody to Carbonic Anhydrase I (CA1) WB; IHC; ICC; IF.
Assay Kits CLV936Ra01 CLIA Kit for Carbonic Anhydrase I (CA1) CLIA assay for Antigen Detection
ELV936Ra01 ELISA Kit for Carbonic Anhydrase I (CA1) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Canis familiaris; Canine (Dog)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPV936Ca01 Recombinant Carbonic Anhydrase I (CA1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAV936Ca01 Monoclonal Antibody to Carbonic Anhydrase I (CA1) WB; IHC; ICC; IP.
PAV936Ca01 Polyclonal Antibody to Carbonic Anhydrase I (CA1) WB; IHC; ICC; IF.
Assay Kits CLV936Ca01 CLIA Kit for Carbonic Anhydrase I (CA1) CLIA assay for Antigen Detection
ELV936Ca01 ELISA Kit for Carbonic Anhydrase I (CA1) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Sus scrofa; Porcine (Pig)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPV936Po01 Recombinant Carbonic Anhydrase I (CA1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAV936Po01 Monoclonal Antibody to Carbonic Anhydrase I (CA1) WB; IHC; ICC; IP.
PAV936Po01 Polyclonal Antibody to Carbonic Anhydrase I (CA1) WB; IHC; ICC; IF.
Assay Kits CLV936Po01 CLIA Kit for Carbonic Anhydrase I (CA1) CLIA assay for Antigen Detection
ELV936Po01 ELISA Kit for Carbonic Anhydrase I (CA1) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Bos taurus; Bovine (Cattle)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPV936Bo01 Recombinant Carbonic Anhydrase I (CA1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAV936Bo01 Monoclonal Antibody to Carbonic Anhydrase I (CA1) WB; IHC; ICC; IP.
PAV936Bo01 Polyclonal Antibody to Carbonic Anhydrase I (CA1) WB; IHC; ICC; IF.
Assay Kits CLV936Bo01 CLIA Kit for Carbonic Anhydrase I (CA1) CLIA assay for Antigen Detection
ELV936Bo01 ELISA Kit for Carbonic Anhydrase I (CA1) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Oryctolagus cuniculus (Rabbit)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPV936Rb01 Recombinant Carbonic Anhydrase I (CA1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAV936Rb01 Monoclonal Antibody to Carbonic Anhydrase I (CA1) WB; IHC; ICC; IP.
PAV936Rb01 Polyclonal Antibody to Carbonic Anhydrase I (CA1) WB; IHC; ICC; IF.
Assay Kits CLV936Rb01 CLIA Kit for Carbonic Anhydrase I (CA1) CLIA assay for Antigen Detection
ELV936Rb01 ELISA Kit for Carbonic Anhydrase I (CA1) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Rhesus monkey (Simian)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPV936Mo01 Recombinant Carbonic Anhydrase I (CA1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAV936Mo01 Monoclonal Antibody to Carbonic Anhydrase I (CA1) WB; IHC; ICC; IP.
PAV936Mo01 Polyclonal Antibody to Carbonic Anhydrase I (CA1) WB; IHC; ICC; IF.
Assay Kits CLV936Mo01 CLIA Kit for Carbonic Anhydrase I (CA1) CLIA assay for Antigen Detection
ELV936Mo01 ELISA Kit for Carbonic Anhydrase I (CA1) Enzyme-linked immunosorbent assay for Antigen Detection

Reference

"Human carbonic anhydrase I cDNA."
Nucleic Acids Res. 15:2386-2386(1987) [PubMed] [Europe PMC] [Abstract]

"Structure and methylation patterns of the gene encoding human carbonic anhydrase I."
Gene 93:277-283(1990) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Primary structure of human B erythrocyte carbonic anhydrase. 3. Sequence of CNBr fragment I and III (residues 149-260)."
Biochimie 56:1031-1043(1974) [PubMed] [Europe PMC] [Abstract]

"Amino acid sequence of human erythrocyte carbonic anhydrase B."
Biochem. Biophys. Res. Commun. 48:670-677(1972) [PubMed] [Europe PMC] [Abstract]

"Human carbonic anhydrases. XI. The complete primary structure of carbonic anhydrase B."
J. Biol. Chem. 248:1885-1893(1973) [PubMed] [Europe PMC] [Abstract]

"Human carbonic anhydrases. XII. The complete primary structure of the C isozyme."
J. Biol. Chem. 249:2329-2337(1974) [PubMed] [Europe PMC] [Abstract]

"Carbonic anhydrase catalyzes cyanamide hydration to urea: is it mimicking the physiological reaction?"
J. Biol. Inorg. Chem. 4:528-536(1999) [PubMed] [Europe PMC] [Abstract]

"Carbonic anhydrase activators. Activation of isozymes I, II, IV, VA, VII, and XIV with l- and d-histidine and crystallographic analysis of their adducts with isoform II: engineering proton-transfer processes within the active site of an enzyme."
Chemistry 12:7057-7066(2006) [PubMed] [Europe PMC] [Abstract]

"Carbonic anhydrase activators. Activation of isoforms I, II, IV, VA, VII, and XIV with L- and D-phenylalanine and crystallographic analysis of their adducts with isozyme II: stereospecific recognition within the active site of an enzyme and its consequences for the drug design."
J. Med. Chem. 49:3019-3027(2006) [PubMed] [Europe PMC] [Abstract]

"Carbonic anhydrase activators: L-Adrenaline plugs the active site entrance of isozyme II, activating better isoforms I, IV, VA, VII, and XIV."
Bioorg. Med. Chem. Lett. 17:628-635(2007) [PubMed] [Europe PMC] [Abstract]

"A thiabendazole sulfonamide shows potent inhibitory activity against mammalian and nematode alpha-carbonic anhydrases."
Bioorg. Med. Chem. Lett. 19:1371-1375(2009) [PubMed] [Europe PMC] [Abstract]

"Non-zinc mediated inhibition of carbonic anhydrases: coumarins are a new class of suicide inhibitors."
J. Am. Chem. Soc. 131:3057-3062(2009) [PubMed] [Europe PMC] [Abstract]

"Crystal structure of human carbonic anhydrase XIII and its complex with the inhibitor acetazolamide."
Proteins 74:164-175(2009) [PubMed] [Europe PMC] [Abstract]

"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]

"Structure of human carbonic anhydrase B. I. Crystallization and heavy atom modifications."
J. Mol. Biol. 63:601-604(1972) [PubMed] [Europe PMC] [Abstract]

"Crystal structure of human erythrocyte carbonic anhydrase B. Three-dimensional structure at a nominal 2.2-A resolution."
Proc. Natl. Acad. Sci. U.S.A. 72:51-55(1975) [PubMed] [Europe PMC] [Abstract]

"Structure, refinement, and function of carbonic anhydrase isozymes: refinement of human carbonic anhydrase I."
Ann. N. Y. Acad. Sci. 429:49-60(1984) [PubMed] [Europe PMC] [Abstract]

"Differences in anionic inhibition of human carbonic anhydrase I revealed from the structures of iodide and gold cyanide inhibitor complexes."
Acta Crystallogr. D 50:731-738(1994) [PubMed] [Europe PMC] [Abstract]

"Enzyme-substrate interactions. Structure of human carbonic anhydrase I complexed with bicarbonate."
J. Mol. Biol. 241:226-232(1994) [PubMed] [Europe PMC] [Abstract]

"Drug-protein interactions. Refined structures of three sulfonamide drug complexes of human carbonic anhydrase I enzyme."
J. Mol. Biol. 243:298-309(1994) [PubMed] [Europe PMC] [Abstract]

"Crystal structure of a zinc-activated variant of human carbonic anhydrase I, CA I Michigan 1: evidence for a second zinc binding site involving arginine coordination."
Biochemistry 41:6237-6244(2002) [PubMed] [Europe PMC] [Abstract]

"Carbonic anhydrase activators: the first X-ray crystallographic study of an adduct of isoform I."
Bioorg. Med. Chem. Lett. 16:5152-5156(2006) [PubMed] [Europe PMC] [Abstract]

"Ultrahigh resolution crystal structures of human carbonic anhydrases I and II complexed with 'two-prong' inhibitors reveal the molecular basis of high affinity."
J. Am. Chem. Soc. 128:3011-3018(2006) [PubMed] [Europe PMC] [Abstract]

"Phosph(on)ate as a zinc-binding group in metalloenzyme inhibitors: X-ray crystal structure of the antiviral drug foscarnet complexed to human carbonic anhydrase I."
Bioorg. Med. Chem. Lett. 17:2210-2215(2007) [PubMed] [Europe PMC] [Abstract]

"Structural analysis of charge discrimination in the binding of inhibitors to human carbonic anhydrases I and II."
J. Am. Chem. Soc. 129:5528-5537(2007) [PubMed] [Europe PMC] [Abstract]

"Population genetic studies of the Philippine Negritos. III. Identification of the carbonic anhydrase-1 variant with CA1 Guam."
Am. J. Hum. Genet. 33:105-111(1981) [PubMed] [Europe PMC] [Abstract]

"Marked zinc activation of ester hydrolysis by a mutation, 67-His (CAT) to Arg (CGT), in the active site of human carbonic anhydrase I."
Hum. Mutat. 4:294-296(1994) [PubMed] [Europe PMC] [Abstract]