Alkaline Phosphatase, Intestinal (ALPI)

CIP; IAP; Calf Intestinal Alkaline Phosphatase

SPN	V728
HGNC	437
MGI	87984
PubMed	20536777
RGD	2099
UniProt	P09923
Wiki	ALPI

Alkaline phosphatase, intestinal also known as ALPI is a type of alkaline phosphatase.The deduced polypeptide showed 86.5% amino acid identity to placental ALP and 56.6% amino acid identity to liver/bone/kidney ALP. Thus, the immunologic cross-reactivity of ALPI and ALPP is perhaps explained.

Calf Intestinal Alkaline Phosphatase (CIP) is an enzyme that catalyzes the 5' phosphate group's removal from DNA. This enzyme is frequently used in DNA sub-cloning, because DNA fragments which lack the 5' phosphoryl termini cannot self-ligate. Preventing self ligation is important both in improving the yield of properly ligated product, and reducing the background of improperly self-ligated contaminant.

Reference

"Cloning and sequencing of human intestinal alkaline phosphatase cDNA."
Proc. Natl. Acad. Sci. U.S.A. 84:695-698(1987) [PubMed] [Europe PMC] [Abstract]

"Nucleotide and amino acid sequences of human intestinal alkaline phosphatase: close homology to placental alkaline phosphatase."
Proc. Natl. Acad. Sci. U.S.A. 84:1234-1238(1987) [PubMed] [Europe PMC] [Abstract]

"Sequence and characterization of the human intestinal alkaline phosphatase gene."
J. Biol. Chem. 263:12011-12019(1988) [PubMed] [Europe PMC] [Abstract]

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Promoter structure of the human intestinal alkaline phosphatase gene."
Nucleic Acids Res. 15:10599-10599(1987) [PubMed] [Europe PMC] [Abstract]

"Two gene duplication events in the evolution of the human heat-stable alkaline phosphatases."
Gene 60:267-276(1987) [PubMed] [Europe PMC] [Abstract]

"Partial sequencing of human adult, human fetal, and bovine intestinal alkaline phosphatases: comparison with the human placental and liver isozymes."
Proc. Natl. Acad. Sci. U.S.A. 83:2368-2372(1986) [PubMed] [Europe PMC] [Abstract]

"Chemical nature of intestinal-type alkaline phosphatase in human kidney."
Clin. Chem. 38:2539-2542(1992) [PubMed] [Europe PMC] [Abstract]

"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]