Centromere Protein B (CENPB)
CENP-B; Major centromere autoantigen B
Centromere Protein B is a highly conserved protein that facilitates centromere formation. It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. The DNA binding domain recognizes and binds a 17-bp sequence (CENP-B box) in the centromeric alpha satellite DNA.
This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies.
Organism species: Homo sapiens (Human)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | V688Hu01 | Recombinant Centromere Protein B (CENPB) | Positive Control; Immunogen; SDS-PAGE; WB. |
| NONE | Native Centromere Protein B (CENPB) | Bioactivity | |
| Antibodies | NONE | Monoclonal Antibody to Centromere Protein B (CENPB) | WB; IHC; ICC; IP. |
| PV688Hu01 | Polyclonal Antibody to Centromere Protein B (CENPB) | WB; IHC; ICC; IF. | |
| NONE | Biotin Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | ELISA(Det);IC; IH; IH(P); WB | |
| NONE | APC Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | IHC, WB | |
| NONE | HRP Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | Simple Western; WB | |
| NONE | PE Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | Flow Cytometry | |
| NONE | FITC Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | Flow Cytometry;ICC/IF | |
| Assay Kits | NONE | ELISA Kit for Centromere Protein B (CENPB) | Enzyme-linked immunosorbent assay for Antigen Detection |
| NONE | CLIA kit for Centromere Protein B (CENPB) | CLIA assay for Antigen Detection |
Organism species: Mus musculus (Mouse)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | NONE | Recombinant Centromere Protein B (CENPB) | Positive Control; Immunogen; SDS-PAGE; WB. |
| NONE | Native Centromere Protein B (CENPB) | Bioactivity | |
| Antibodies | NONE | Monoclonal Antibody to Centromere Protein B (CENPB) | WB; IHC; ICC; IP. |
| NONE | Polyclonal Antibody to Centromere Protein B (CENPB) | WB; IHC; ICC; IF. | |
| NONE | Biotin Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | ELISA(Det);IC; IH; IH(P); WB | |
| NONE | APC Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | IHC, WB | |
| NONE | HRP Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | Simple Western; WB | |
| NONE | PE Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | Flow Cytometry | |
| NONE | FITC Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | Flow Cytometry;ICC/IF | |
| Assay Kits | NONE | ELISA Kit for Centromere Protein B (CENPB) | Enzyme-linked immunosorbent assay for Antigen Detection |
| NONE | CLIA kit for Centromere Protein B (CENPB) | CLIA assay for Antigen Detection |
Organism species: Rattus norvegicus (Rat)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | NONE | Recombinant Centromere Protein B (CENPB) | Positive Control; Immunogen; SDS-PAGE; WB. |
| NONE | Native Centromere Protein B (CENPB) | Bioactivity | |
| Antibodies | NONE | Monoclonal Antibody to Centromere Protein B (CENPB) | WB; IHC; ICC; IP. |
| NONE | Polyclonal Antibody to Centromere Protein B (CENPB) | WB; IHC; ICC; IF. | |
| NONE | Biotin Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | ELISA(Det);IC; IH; IH(P); WB | |
| NONE | APC Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | IHC, WB | |
| NONE | HRP Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | Simple Western; WB | |
| NONE | PE Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | Flow Cytometry | |
| NONE | FITC Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | Flow Cytometry;ICC/IF | |
| Assay Kits | NONE | ELISA Kit for Centromere Protein B (CENPB) | Enzyme-linked immunosorbent assay for Antigen Detection |
| NONE | CLIA kit for Centromere Protein B (CENPB) | CLIA assay for Antigen Detection |
Organism species: Canis familiaris; Canine (Dog)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | NONE | Recombinant Centromere Protein B (CENPB) | Positive Control; Immunogen; SDS-PAGE; WB. |
| NONE | Native Centromere Protein B (CENPB) | Bioactivity | |
| Antibodies | NONE | Monoclonal Antibody to Centromere Protein B (CENPB) | WB; IHC; ICC; IP. |
| NONE | Polyclonal Antibody to Centromere Protein B (CENPB) | WB; IHC; ICC; IF. | |
| NONE | Biotin Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | ELISA(Det);IC; IH; IH(P); WB | |
| NONE | APC Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | IHC, WB | |
| NONE | HRP Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | Simple Western; WB | |
| NONE | PE Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | Flow Cytometry | |
| NONE | FITC Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | Flow Cytometry;ICC/IF | |
| Assay Kits | NONE | ELISA Kit for Centromere Protein B (CENPB) | Enzyme-linked immunosorbent assay for Antigen Detection |
| NONE | CLIA kit for Centromere Protein B (CENPB) | CLIA assay for Antigen Detection |
Organism species: Sus scrofa; Porcine (Pig)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | NONE | Recombinant Centromere Protein B (CENPB) | Positive Control; Immunogen; SDS-PAGE; WB. |
| NONE | Native Centromere Protein B (CENPB) | Bioactivity | |
| Antibodies | NONE | Monoclonal Antibody to Centromere Protein B (CENPB) | WB; IHC; ICC; IP. |
| NONE | Polyclonal Antibody to Centromere Protein B (CENPB) | WB; IHC; ICC; IF. | |
| NONE | Biotin Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | ELISA(Det);IC; IH; IH(P); WB | |
| NONE | APC Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | IHC, WB | |
| NONE | HRP Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | Simple Western; WB | |
| NONE | PE Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | Flow Cytometry | |
| NONE | FITC Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | Flow Cytometry;ICC/IF | |
| Assay Kits | NONE | ELISA Kit for Centromere Protein B (CENPB) | Enzyme-linked immunosorbent assay for Antigen Detection |
| NONE | CLIA kit for Centromere Protein B (CENPB) | CLIA assay for Antigen Detection |
Organism species: Bos taurus; Bovine (Cattle)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | NONE | Recombinant Centromere Protein B (CENPB) | Positive Control; Immunogen; SDS-PAGE; WB. |
| NONE | Native Centromere Protein B (CENPB) | Bioactivity | |
| Antibodies | NONE | Monoclonal Antibody to Centromere Protein B (CENPB) | WB; IHC; ICC; IP. |
| NONE | Polyclonal Antibody to Centromere Protein B (CENPB) | WB; IHC; ICC; IF. | |
| NONE | Biotin Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | ELISA(Det);IC; IH; IH(P); WB | |
| NONE | APC Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | IHC, WB | |
| NONE | HRP Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | Simple Western; WB | |
| NONE | PE Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | Flow Cytometry | |
| NONE | FITC Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | Flow Cytometry;ICC/IF | |
| Assay Kits | NONE | ELISA Kit for Centromere Protein B (CENPB) | Enzyme-linked immunosorbent assay for Antigen Detection |
| NONE | CLIA kit for Centromere Protein B (CENPB) | CLIA assay for Antigen Detection |
Organism species: Rhesus monkey (Simian)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | NONE | Recombinant Centromere Protein B (CENPB) | Positive Control; Immunogen; SDS-PAGE; WB. |
| NONE | Native Centromere Protein B (CENPB) | Bioactivity | |
| Antibodies | NONE | Monoclonal Antibody to Centromere Protein B (CENPB) | WB; IHC; ICC; IP. |
| NONE | Polyclonal Antibody to Centromere Protein B (CENPB) | WB; IHC; ICC; IF. | |
| NONE | Biotin Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | ELISA(Det);IC; IH; IH(P); WB | |
| NONE | APC Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | IHC, WB | |
| NONE | HRP Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | Simple Western; WB | |
| NONE | PE Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | Flow Cytometry | |
| NONE | FITC Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | Flow Cytometry;ICC/IF | |
| Assay Kits | NONE | ELISA Kit for Centromere Protein B (CENPB) | Enzyme-linked immunosorbent assay for Antigen Detection |
| NONE | CLIA kit for Centromere Protein B (CENPB) | CLIA assay for Antigen Detection |
Organism species: Chicken (Gallus)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | NONE | Recombinant Centromere Protein B (CENPB) | Positive Control; Immunogen; SDS-PAGE; WB. |
| NONE | Native Centromere Protein B (CENPB) | Bioactivity | |
| Antibodies | NONE | Monoclonal Antibody to Centromere Protein B (CENPB) | WB; IHC; ICC; IP. |
| NONE | Polyclonal Antibody to Centromere Protein B (CENPB) | WB; IHC; ICC; IF. | |
| NONE | Biotin Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | ELISA(Det);IC; IH; IH(P); WB | |
| NONE | APC Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | IHC, WB | |
| NONE | HRP Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | Simple Western; WB | |
| NONE | PE Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | Flow Cytometry | |
| NONE | FITC Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | Flow Cytometry;ICC/IF | |
| Assay Kits | NONE | ELISA Kit for Centromere Protein B (CENPB) | Enzyme-linked immunosorbent assay for Antigen Detection |
| NONE | CLIA kit for Centromere Protein B (CENPB) | CLIA assay for Antigen Detection |
Organism species: Ovis aries; Ovine (Sheep)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | NONE | Recombinant Centromere Protein B (CENPB) | Positive Control; Immunogen; SDS-PAGE; WB. |
| NONE | Native Centromere Protein B (CENPB) | Bioactivity | |
| Antibodies | NONE | Monoclonal Antibody to Centromere Protein B (CENPB) | WB; IHC; ICC; IP. |
| NONE | Polyclonal Antibody to Centromere Protein B (CENPB) | WB; IHC; ICC; IF. | |
| NONE | Biotin Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | ELISA(Det);IC; IH; IH(P); WB | |
| NONE | APC Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | IHC, WB | |
| NONE | HRP Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | Simple Western; WB | |
| NONE | PE Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | Flow Cytometry | |
| NONE | FITC Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | Flow Cytometry;ICC/IF | |
| Assay Kits | NONE | ELISA Kit for Centromere Protein B (CENPB) | Enzyme-linked immunosorbent assay for Antigen Detection |
| NONE | CLIA kit for Centromere Protein B (CENPB) | CLIA assay for Antigen Detection |
Organism species: Oryctolagus cuniculus (Rabbit)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | NONE | Recombinant Centromere Protein B (CENPB) | Positive Control; Immunogen; SDS-PAGE; WB. |
| NONE | Native Centromere Protein B (CENPB) | Bioactivity | |
| Antibodies | NONE | Monoclonal Antibody to Centromere Protein B (CENPB) | WB; IHC; ICC; IP. |
| NONE | Polyclonal Antibody to Centromere Protein B (CENPB) | WB; IHC; ICC; IF. | |
| NONE | Biotin Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | ELISA(Det);IC; IH; IH(P); WB | |
| NONE | APC Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | IHC, WB | |
| NONE | HRP Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | Simple Western; WB | |
| NONE | PE Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | Flow Cytometry | |
| NONE | FITC Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | Flow Cytometry;ICC/IF | |
| Assay Kits | NONE | ELISA Kit for Centromere Protein B (CENPB) | Enzyme-linked immunosorbent assay for Antigen Detection |
| NONE | CLIA kit for Centromere Protein B (CENPB) | CLIA assay for Antigen Detection |
Organism species: Cavia (Guinea pig )
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | NONE | Recombinant Centromere Protein B (CENPB) | Positive Control; Immunogen; SDS-PAGE; WB. |
| NONE | Native Centromere Protein B (CENPB) | Bioactivity | |
| Antibodies | NONE | Monoclonal Antibody to Centromere Protein B (CENPB) | WB; IHC; ICC; IP. |
| NONE | Polyclonal Antibody to Centromere Protein B (CENPB) | WB; IHC; ICC; IF. | |
| NONE | Biotin Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | ELISA(Det);IC; IH; IH(P); WB | |
| NONE | APC Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | IHC, WB | |
| NONE | HRP Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | Simple Western; WB | |
| NONE | PE Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | Flow Cytometry | |
| NONE | FITC Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | Flow Cytometry;ICC/IF | |
| Assay Kits | NONE | ELISA Kit for Centromere Protein B (CENPB) | Enzyme-linked immunosorbent assay for Antigen Detection |
| NONE | CLIA kit for Centromere Protein B (CENPB) | CLIA assay for Antigen Detection |
Organism species: Equus caballus; Equine (Horse)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | NONE | Recombinant Centromere Protein B (CENPB) | Positive Control; Immunogen; SDS-PAGE; WB. |
| NONE | Native Centromere Protein B (CENPB) | Bioactivity | |
| Antibodies | NONE | Monoclonal Antibody to Centromere Protein B (CENPB) | WB; IHC; ICC; IP. |
| NONE | Polyclonal Antibody to Centromere Protein B (CENPB) | WB; IHC; ICC; IF. | |
| NONE | Biotin Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | ELISA(Det);IC; IH; IH(P); WB | |
| NONE | APC Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | IHC, WB | |
| NONE | HRP Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | Simple Western; WB | |
| NONE | PE Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | Flow Cytometry | |
| NONE | FITC Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | Flow Cytometry;ICC/IF | |
| Assay Kits | NONE | ELISA Kit for Centromere Protein B (CENPB) | Enzyme-linked immunosorbent assay for Antigen Detection |
| NONE | CLIA kit for Centromere Protein B (CENPB) | CLIA assay for Antigen Detection |
Organism species: Capra hircus; Caprine (Goat)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | NONE | Recombinant Centromere Protein B (CENPB) | Positive Control; Immunogen; SDS-PAGE; WB. |
| NONE | Native Centromere Protein B (CENPB) | Bioactivity | |
| Antibodies | NONE | Monoclonal Antibody to Centromere Protein B (CENPB) | WB; IHC; ICC; IP. |
| NONE | Polyclonal Antibody to Centromere Protein B (CENPB) | WB; IHC; ICC; IF. | |
| NONE | Biotin Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | ELISA(Det);IC; IH; IH(P); WB | |
| NONE | APC Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | IHC, WB | |
| NONE | HRP Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | Simple Western; WB | |
| NONE | PE Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | Flow Cytometry | |
| NONE | FITC Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | Flow Cytometry;ICC/IF | |
| Assay Kits | NONE | ELISA Kit for Centromere Protein B (CENPB) | Enzyme-linked immunosorbent assay for Antigen Detection |
| NONE | CLIA kit for Centromere Protein B (CENPB) | CLIA assay for Antigen Detection |
Organism species: Pan-species (General)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | NONE | Recombinant Centromere Protein B (CENPB) | Positive Control; Immunogen; SDS-PAGE; WB. |
| NONE | Native Centromere Protein B (CENPB) | Bioactivity | |
| Antibodies | NONE | Monoclonal Antibody to Centromere Protein B (CENPB) | WB; IHC; ICC; IP. |
| NONE | Polyclonal Antibody to Centromere Protein B (CENPB) | WB; IHC; ICC; IF. | |
| NONE | Biotin Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | ELISA(Det);IC; IH; IH(P); WB | |
| NONE | APC Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | IHC, WB | |
| NONE | HRP Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | Simple Western; WB | |
| NONE | PE Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | Flow Cytometry | |
| NONE | FITC Linked-Polyclonal Antibody to Centromere Protein B (CENPB) | Flow Cytometry;ICC/IF | |
| Assay Kits | NONE | ELISA Kit for Centromere Protein B (CENPB) | Enzyme-linked immunosorbent assay for Antigen Detection |
| NONE | CLIA kit for Centromere Protein B (CENPB) | CLIA assay for Antigen Detection |
Reference
"CENP-B is a highly conserved mammalian centromere protein with homology to the helix-loop-helix family of proteins."
Chromosoma 100:360-370(1991) [PubMed] [Europe PMC] [Abstract]
"The DNA sequence and comparative analysis of human chromosome 20."
Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
"Molecular cloning of cDNA for CENP-B, the major human centromere autoantigen."
J. Cell Biol. 104:817-829(1987) [PubMed] [Europe PMC] [Abstract]
"A human centromere protein, CENP-B, has a DNA binding domain containing four potential alpha helices at the NH2 terminus, which is separable from dimerizing activity."
J. Cell Biol. 119:1413-1427(1992) [PubMed] [Europe PMC] [Abstract]
"Assembly of the inner kinetochore proteins CENP-A and CENP-B in living human cells."
ChemBioChem 9:77-92(2008) [PubMed] [Europe PMC] [Abstract]
"Live-cell imaging reveals sustained centromere binding of CENP-T via CENP-A and CENP-B."
J. Biophotonics 1:245-254(2008) [PubMed] [Europe PMC] [Abstract]
"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
"Identification of novel alpha-n-methylation of CENP-B that regulates its binding to the centromeric DNA."
J. Proteome Res. 12:4167-4175(2013) [PubMed] [Europe PMC] [Abstract]
"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
"A helix-turn-helix structure unit in human centromere protein B (CENP-B)."
EMBO J. 17:827-837(1998) [PubMed] [Europe PMC] [Abstract]
"Crystal structure of the CENP-B protein-DNA complex: the DNA-binding domains of CENP-B induce kinks in the CENP-B box DNA."
EMBO J. 20:6612-6618(2001) [PubMed] [Europe PMC] [Abstract]
"Crystal structure of the human centromere protein B (CENP-B) dimerization domain at 1.65-A resolution."
J. Biol. Chem. 278:51454-51461(2003) [PubMed] [Europe PMC] [Abstract]