Lymphocyte Cytosolic Protein 2 (LCP2)

SLP76; SH2 Domain Containing Leukocyte Protein Of 76kDa; 76 kDa Tyrosine Phosphoprotein

SPN	V619
HGNC	6529
MGI	1321402
PubMed	8661136
RGD	619743
UniProt	Q13094
Wiki	LCP2

SLP-76 was originally identified as a substrate of the ZAP-70 protein tyrosine kinase following T cell receptor (TCR) ligation in the leukemic T cell line Jurkat. The SLP-76 locus has been localized to human chromosome 5q33 and the gene structure has been partially characterized in mice. The human and murine cDNAs both encode 533 amino acid proteins that are 72% identical and comprised of three modular domains. The NH2-terminus contains an acidic region that includes a PEST domain and several tyrosine residues which are phosphorylated following TCR ligation.A number of additional proteins have been identified that associate with SLP-76 both constitutively and inducibly following receptor ligation, supporting the notion that SLP-76 functions as an adaptor or scaffold protein.

Reference

"Molecular cloning of SLP-76, a 76-kDa tyrosine phosphoprotein associated with Grb2 in T cells."
J. Biol. Chem. 270:7029-7032(1995) [PubMed] [Europe PMC] [Abstract]

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"PRAM-1 is a novel adaptor protein regulated by retinoic acid (RA) and promyelocytic leukemia (PML)-RA receptor alpha in acute promyelocytic leukemia cells."
J. Biol. Chem. 276:22375-22381(2001) [PubMed] [Europe PMC] [Abstract]

"Shb links SLP-76 and Vav with the CD3 complex in Jurkat T cells."
Eur. J. Biochem. 269:3279-3288(2002) [PubMed] [Europe PMC] [Abstract]

"Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry."
Anal. Chem. 76:2763-2772(2004) [PubMed] [Europe PMC] [Abstract]

"Association of the Src homology 2 domain-containing leukocyte phosphoprotein of 76 kD (SLP-76) with the p85 subunit of phosphoinositide 3-kinase."
FEBS Lett. 575:35-40(2004) [PubMed] [Europe PMC] [Abstract]

"Phosphoproteome of resting human platelets."
J. Proteome Res. 7:526-534(2008) [PubMed] [Europe PMC] [Abstract]

"Sequential phosphorylation of SLP-76 at tyrosine 173 is required for activation of T and mast cells."
EMBO J. 30:3160-3172(2011) [PubMed] [Europe PMC] [Abstract]

"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]

"Solution structure of the N-terminal SAM-domain of human lymphocyte cytosolic protein 2."
Submitted (FEB-2008) to the PDB data bank