Dopamine Beta Hydroxylase (DbH)

DBM; Dopamine Beta-Monooxygenase

SPN	V405
HGNC	2689
MGI	94864
PubMed	20301647
RGD	2489
UniProt	P09172
Wiki	DBH

DBH is a 290 kDa copper-containing oxygenase consisting of four identical subunits, and its activity requires ascorbate as a cofactor. It is the only enzyme involved in the synthesis of small-molecule neurotransmitters that is membrane-bound, making norepinephrine and epinephrine the only transmitters synthesized inside vesicles. It is expressed in noradrenergic nerve terminals of the central and peripheral nervous systems, as well as in chromaffin cells of the adrenal medulla.DBH is inhibited by disulfiram, tropolone, and, most selectively, by nepicastat. DBH exists in both membrane-bound and soluble forms. Taken together with the biochemical data, the observations suggested that the membrane attachment of DBH probably results from a posttranslational modification, glypiation being the most likely candidate.

Reference

"DNA sequence and analysis of human chromosome 9."
Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]

"The primary structure of human dopamine-beta-hydroxylase: insights into the relationship between the soluble and the membrane-bound forms of the enzyme."
EMBO J. 6:3931-3937(1987) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Human dopamine beta-hydroxylase gene: two mRNA types having different 3'-terminal regions are produced through alternative polyadenylation."
Nucleic Acids Res. 17:1089-1102(1989) [PubMed] [Europe PMC] [Abstract]

"Expression of human dopamine beta-hydroxylase in Drosophila Schneider 2 cells."
Biochem. J. 313:57-64(1996) [PubMed] [Europe PMC] [Abstract]

"Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry."
J. Proteome Res. 4:2070-2080(2005) [PubMed] [Europe PMC] [Abstract]

"A strategy for precise and large scale identification of core fucosylated glycoproteins."
Mol. Cell. Proteomics 8:913-923(2009) [PubMed] [Europe PMC] [Abstract]

"No evidence for allelic association between schizophrenia and a functional variant of the human dopamine beta-hydroxylase gene (DBH)."
Am. J. Med. Genet. 88:557-559(1999) [PubMed] [Europe PMC] [Abstract]

"Characterization of single-nucleotide polymorphisms in coding regions of human genes."
Nat. Genet. 22:231-238(1999) [PubMed] [Europe PMC] [Abstract]

Erratum
Nat. Genet. 23:373-373(1999)

"Patterns of single-nucleotide polymorphisms in candidate genes for blood-pressure homeostasis."
Nat. Genet. 22:239-247(1999) [PubMed] [Europe PMC] [Abstract]

"Mutations in the dopamine beta-hydroxylase gene are associated with human norepinephrine deficiency."
Am. J. Med. Genet. 108:140-147(2002) [PubMed] [Europe PMC] [Abstract]