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Proteinase 3 (PR3)

MBN; PRTN3; ACPA; AGP7; C-ANCA; MBT; P29; PRK; Myeloblastin; Serine Proteinase,Neutrophil,Wegener Granulomatosis Autoantigen; Protease K; Proteinase K; Endopeptidase K

PRTN3 is a serine protease enzyme expressed mainly in neutrophil granulocytes. Its exact role in the function of the neutrophil is unknown but in human neutrophils proteinase 3 contributes to the proteolytic generation of antimicrobial peptides. It is also the epitope of anti-neutrophil cytoplasmic antibodies (ANCAs) of the cANCA (cytoplasmic subtype) class, a type of antibody frequently found in the disease Wegener's granulomatosis.

Proteinase K is commonly used in molecular biology to digest protein and remove contamination from preparations of nucleic acid. Addition of proteinase K to nucleic acid preparations rapidly inactivates nucleases that might otherwise degrade the DNA or RNA during purification.

Organism species: Homo sapiens (Human)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins V400Hu01 Recombinant Proteinase 3 (PR3) Positive Control; Immunogen; SDS-PAGE; WB.
NONE Native Proteinase 3 (PR3) Bioactivity
Antibodies MV400Hu22 Monoclonal Antibody to Proteinase 3 (PR3) WB; IHC; ICC; IP.
PV400Hu01 Polyclonal Antibody to Proteinase 3 (PR3) WB; IHC; ICC; IF.
NONE Biotin Linked-Polyclonal Antibody to Proteinase 3 (PR3) ELISA(Det);IC; IH; IH(P); WB
NONE APC Linked-Polyclonal Antibody to Proteinase 3 (PR3) IHC, WB
NONE HRP Linked-Polyclonal Antibody to Proteinase 3 (PR3) Simple Western; WB
NONE PE Linked-Polyclonal Antibody to Proteinase 3 (PR3) Flow Cytometry
NONE FITC Linked-Polyclonal Antibody to Proteinase 3 (PR3) Flow Cytometry;ICC/IF
Assay Kits KEV400Hu ELISA Kit for Proteinase 3 (PR3) Enzyme-linked immunosorbent assay for Antigen Detection
NONE CLIA kit for Proteinase 3 (PR3) CLIA assay for Antigen Detection

Organism species: Mus musculus (Mouse)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins V400Mu01 Recombinant Proteinase 3 (PR3) Positive Control; Immunogen; SDS-PAGE; WB.
NONE Native Proteinase 3 (PR3) Bioactivity
Antibodies NONE Monoclonal Antibody to Proteinase 3 (PR3) WB; IHC; ICC; IP.
PV400Mu01 Polyclonal Antibody to Proteinase 3 (PR3) WB; IHC; ICC; IF.
NONE Biotin Linked-Polyclonal Antibody to Proteinase 3 (PR3) ELISA(Det);IC; IH; IH(P); WB
NONE APC Linked-Polyclonal Antibody to Proteinase 3 (PR3) IHC, WB
NONE HRP Linked-Polyclonal Antibody to Proteinase 3 (PR3) Simple Western; WB
NONE PE Linked-Polyclonal Antibody to Proteinase 3 (PR3) Flow Cytometry
NONE FITC Linked-Polyclonal Antibody to Proteinase 3 (PR3) Flow Cytometry;ICC/IF
Assay Kits KEV400Mu ELISA Kit for Proteinase 3 (PR3) Enzyme-linked immunosorbent assay for Antigen Detection
NONE CLIA kit for Proteinase 3 (PR3) CLIA assay for Antigen Detection

Organism species: Rattus norvegicus (Rat)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins V400Ra01 Recombinant Proteinase 3 (PR3) Positive Control; Immunogen; SDS-PAGE; WB.
NONE Native Proteinase 3 (PR3) Bioactivity
Antibodies MV400Ra21 Monoclonal Antibody to Proteinase 3 (PR3) WB; IHC; ICC; IP.
PV400Ra01 Polyclonal Antibody to Proteinase 3 (PR3) WB; IHC; ICC; IF.
NONE Biotin Linked-Polyclonal Antibody to Proteinase 3 (PR3) ELISA(Det);IC; IH; IH(P); WB
NONE APC Linked-Polyclonal Antibody to Proteinase 3 (PR3) IHC, WB
NONE HRP Linked-Polyclonal Antibody to Proteinase 3 (PR3) Simple Western; WB
NONE PE Linked-Polyclonal Antibody to Proteinase 3 (PR3) Flow Cytometry
NONE FITC Linked-Polyclonal Antibody to Proteinase 3 (PR3) Flow Cytometry;ICC/IF
Assay Kits KEV400Ra ELISA Kit for Proteinase 3 (PR3) Enzyme-linked immunosorbent assay for Antigen Detection
NONE CLIA kit for Proteinase 3 (PR3) CLIA assay for Antigen Detection

Organism species: Canis familiaris; Canine (Dog)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins NONE Recombinant Proteinase 3 (PR3) Positive Control; Immunogen; SDS-PAGE; WB.
NONE Native Proteinase 3 (PR3) Bioactivity
Antibodies NONE Monoclonal Antibody to Proteinase 3 (PR3) WB; IHC; ICC; IP.
NONE Polyclonal Antibody to Proteinase 3 (PR3) WB; IHC; ICC; IF.
NONE Biotin Linked-Polyclonal Antibody to Proteinase 3 (PR3) ELISA(Det);IC; IH; IH(P); WB
NONE APC Linked-Polyclonal Antibody to Proteinase 3 (PR3) IHC, WB
NONE HRP Linked-Polyclonal Antibody to Proteinase 3 (PR3) Simple Western; WB
NONE PE Linked-Polyclonal Antibody to Proteinase 3 (PR3) Flow Cytometry
NONE FITC Linked-Polyclonal Antibody to Proteinase 3 (PR3) Flow Cytometry;ICC/IF
Assay Kits NONE ELISA Kit for Proteinase 3 (PR3) Enzyme-linked immunosorbent assay for Antigen Detection
NONE CLIA kit for Proteinase 3 (PR3) CLIA assay for Antigen Detection

Organism species: Sus scrofa; Porcine (Pig)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins NONE Recombinant Proteinase 3 (PR3) Positive Control; Immunogen; SDS-PAGE; WB.
NONE Native Proteinase 3 (PR3) Bioactivity
Antibodies NONE Monoclonal Antibody to Proteinase 3 (PR3) WB; IHC; ICC; IP.
NONE Polyclonal Antibody to Proteinase 3 (PR3) WB; IHC; ICC; IF.
NONE Biotin Linked-Polyclonal Antibody to Proteinase 3 (PR3) ELISA(Det);IC; IH; IH(P); WB
NONE APC Linked-Polyclonal Antibody to Proteinase 3 (PR3) IHC, WB
NONE HRP Linked-Polyclonal Antibody to Proteinase 3 (PR3) Simple Western; WB
NONE PE Linked-Polyclonal Antibody to Proteinase 3 (PR3) Flow Cytometry
NONE FITC Linked-Polyclonal Antibody to Proteinase 3 (PR3) Flow Cytometry;ICC/IF
Assay Kits NONE ELISA Kit for Proteinase 3 (PR3) Enzyme-linked immunosorbent assay for Antigen Detection
NONE CLIA kit for Proteinase 3 (PR3) CLIA assay for Antigen Detection

Organism species: Bos taurus; Bovine (Cattle)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins NONE Recombinant Proteinase 3 (PR3) Positive Control; Immunogen; SDS-PAGE; WB.
NONE Native Proteinase 3 (PR3) Bioactivity
Antibodies NONE Monoclonal Antibody to Proteinase 3 (PR3) WB; IHC; ICC; IP.
NONE Polyclonal Antibody to Proteinase 3 (PR3) WB; IHC; ICC; IF.
NONE Biotin Linked-Polyclonal Antibody to Proteinase 3 (PR3) ELISA(Det);IC; IH; IH(P); WB
NONE APC Linked-Polyclonal Antibody to Proteinase 3 (PR3) IHC, WB
NONE HRP Linked-Polyclonal Antibody to Proteinase 3 (PR3) Simple Western; WB
NONE PE Linked-Polyclonal Antibody to Proteinase 3 (PR3) Flow Cytometry
NONE FITC Linked-Polyclonal Antibody to Proteinase 3 (PR3) Flow Cytometry;ICC/IF
Assay Kits NONE ELISA Kit for Proteinase 3 (PR3) Enzyme-linked immunosorbent assay for Antigen Detection
NONE CLIA kit for Proteinase 3 (PR3) CLIA assay for Antigen Detection

Organism species: Rhesus monkey (Simian)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins NONE Recombinant Proteinase 3 (PR3) Positive Control; Immunogen; SDS-PAGE; WB.
NONE Native Proteinase 3 (PR3) Bioactivity
Antibodies NONE Monoclonal Antibody to Proteinase 3 (PR3) WB; IHC; ICC; IP.
NONE Polyclonal Antibody to Proteinase 3 (PR3) WB; IHC; ICC; IF.
NONE Biotin Linked-Polyclonal Antibody to Proteinase 3 (PR3) ELISA(Det);IC; IH; IH(P); WB
NONE APC Linked-Polyclonal Antibody to Proteinase 3 (PR3) IHC, WB
NONE HRP Linked-Polyclonal Antibody to Proteinase 3 (PR3) Simple Western; WB
NONE PE Linked-Polyclonal Antibody to Proteinase 3 (PR3) Flow Cytometry
NONE FITC Linked-Polyclonal Antibody to Proteinase 3 (PR3) Flow Cytometry;ICC/IF
Assay Kits NONE ELISA Kit for Proteinase 3 (PR3) Enzyme-linked immunosorbent assay for Antigen Detection
NONE CLIA kit for Proteinase 3 (PR3) CLIA assay for Antigen Detection

Organism species: Chicken (Gallus)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins NONE Recombinant Proteinase 3 (PR3) Positive Control; Immunogen; SDS-PAGE; WB.
NONE Native Proteinase 3 (PR3) Bioactivity
Antibodies NONE Monoclonal Antibody to Proteinase 3 (PR3) WB; IHC; ICC; IP.
NONE Polyclonal Antibody to Proteinase 3 (PR3) WB; IHC; ICC; IF.
NONE Biotin Linked-Polyclonal Antibody to Proteinase 3 (PR3) ELISA(Det);IC; IH; IH(P); WB
NONE APC Linked-Polyclonal Antibody to Proteinase 3 (PR3) IHC, WB
NONE HRP Linked-Polyclonal Antibody to Proteinase 3 (PR3) Simple Western; WB
NONE PE Linked-Polyclonal Antibody to Proteinase 3 (PR3) Flow Cytometry
NONE FITC Linked-Polyclonal Antibody to Proteinase 3 (PR3) Flow Cytometry;ICC/IF
Assay Kits NONE ELISA Kit for Proteinase 3 (PR3) Enzyme-linked immunosorbent assay for Antigen Detection
NONE CLIA kit for Proteinase 3 (PR3) CLIA assay for Antigen Detection

Organism species: Ovis aries; Ovine (Sheep)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins NONE Recombinant Proteinase 3 (PR3) Positive Control; Immunogen; SDS-PAGE; WB.
NONE Native Proteinase 3 (PR3) Bioactivity
Antibodies NONE Monoclonal Antibody to Proteinase 3 (PR3) WB; IHC; ICC; IP.
NONE Polyclonal Antibody to Proteinase 3 (PR3) WB; IHC; ICC; IF.
NONE Biotin Linked-Polyclonal Antibody to Proteinase 3 (PR3) ELISA(Det);IC; IH; IH(P); WB
NONE APC Linked-Polyclonal Antibody to Proteinase 3 (PR3) IHC, WB
NONE HRP Linked-Polyclonal Antibody to Proteinase 3 (PR3) Simple Western; WB
NONE PE Linked-Polyclonal Antibody to Proteinase 3 (PR3) Flow Cytometry
NONE FITC Linked-Polyclonal Antibody to Proteinase 3 (PR3) Flow Cytometry;ICC/IF
Assay Kits NONE ELISA Kit for Proteinase 3 (PR3) Enzyme-linked immunosorbent assay for Antigen Detection
NONE CLIA kit for Proteinase 3 (PR3) CLIA assay for Antigen Detection

Organism species: Oryctolagus cuniculus (Rabbit)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins NONE Recombinant Proteinase 3 (PR3) Positive Control; Immunogen; SDS-PAGE; WB.
NONE Native Proteinase 3 (PR3) Bioactivity
Antibodies NONE Monoclonal Antibody to Proteinase 3 (PR3) WB; IHC; ICC; IP.
NONE Polyclonal Antibody to Proteinase 3 (PR3) WB; IHC; ICC; IF.
NONE Biotin Linked-Polyclonal Antibody to Proteinase 3 (PR3) ELISA(Det);IC; IH; IH(P); WB
NONE APC Linked-Polyclonal Antibody to Proteinase 3 (PR3) IHC, WB
NONE HRP Linked-Polyclonal Antibody to Proteinase 3 (PR3) Simple Western; WB
NONE PE Linked-Polyclonal Antibody to Proteinase 3 (PR3) Flow Cytometry
NONE FITC Linked-Polyclonal Antibody to Proteinase 3 (PR3) Flow Cytometry;ICC/IF
Assay Kits NONE ELISA Kit for Proteinase 3 (PR3) Enzyme-linked immunosorbent assay for Antigen Detection
NONE CLIA kit for Proteinase 3 (PR3) CLIA assay for Antigen Detection

Organism species: Cavia (Guinea pig )

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins NONE Recombinant Proteinase 3 (PR3) Positive Control; Immunogen; SDS-PAGE; WB.
NONE Native Proteinase 3 (PR3) Bioactivity
Antibodies NONE Monoclonal Antibody to Proteinase 3 (PR3) WB; IHC; ICC; IP.
NONE Polyclonal Antibody to Proteinase 3 (PR3) WB; IHC; ICC; IF.
NONE Biotin Linked-Polyclonal Antibody to Proteinase 3 (PR3) ELISA(Det);IC; IH; IH(P); WB
NONE APC Linked-Polyclonal Antibody to Proteinase 3 (PR3) IHC, WB
NONE HRP Linked-Polyclonal Antibody to Proteinase 3 (PR3) Simple Western; WB
NONE PE Linked-Polyclonal Antibody to Proteinase 3 (PR3) Flow Cytometry
NONE FITC Linked-Polyclonal Antibody to Proteinase 3 (PR3) Flow Cytometry;ICC/IF
Assay Kits NONE ELISA Kit for Proteinase 3 (PR3) Enzyme-linked immunosorbent assay for Antigen Detection
NONE CLIA kit for Proteinase 3 (PR3) CLIA assay for Antigen Detection

Organism species: Equus caballus; Equine (Horse)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins NONE Recombinant Proteinase 3 (PR3) Positive Control; Immunogen; SDS-PAGE; WB.
NONE Native Proteinase 3 (PR3) Bioactivity
Antibodies NONE Monoclonal Antibody to Proteinase 3 (PR3) WB; IHC; ICC; IP.
NONE Polyclonal Antibody to Proteinase 3 (PR3) WB; IHC; ICC; IF.
NONE Biotin Linked-Polyclonal Antibody to Proteinase 3 (PR3) ELISA(Det);IC; IH; IH(P); WB
NONE APC Linked-Polyclonal Antibody to Proteinase 3 (PR3) IHC, WB
NONE HRP Linked-Polyclonal Antibody to Proteinase 3 (PR3) Simple Western; WB
NONE PE Linked-Polyclonal Antibody to Proteinase 3 (PR3) Flow Cytometry
NONE FITC Linked-Polyclonal Antibody to Proteinase 3 (PR3) Flow Cytometry;ICC/IF
Assay Kits NONE ELISA Kit for Proteinase 3 (PR3) Enzyme-linked immunosorbent assay for Antigen Detection
NONE CLIA kit for Proteinase 3 (PR3) CLIA assay for Antigen Detection

Organism species: Capra hircus; Caprine (Goat)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins NONE Recombinant Proteinase 3 (PR3) Positive Control; Immunogen; SDS-PAGE; WB.
NONE Native Proteinase 3 (PR3) Bioactivity
Antibodies NONE Monoclonal Antibody to Proteinase 3 (PR3) WB; IHC; ICC; IP.
NONE Polyclonal Antibody to Proteinase 3 (PR3) WB; IHC; ICC; IF.
NONE Biotin Linked-Polyclonal Antibody to Proteinase 3 (PR3) ELISA(Det);IC; IH; IH(P); WB
NONE APC Linked-Polyclonal Antibody to Proteinase 3 (PR3) IHC, WB
NONE HRP Linked-Polyclonal Antibody to Proteinase 3 (PR3) Simple Western; WB
NONE PE Linked-Polyclonal Antibody to Proteinase 3 (PR3) Flow Cytometry
NONE FITC Linked-Polyclonal Antibody to Proteinase 3 (PR3) Flow Cytometry;ICC/IF
Assay Kits NONE ELISA Kit for Proteinase 3 (PR3) Enzyme-linked immunosorbent assay for Antigen Detection
NONE CLIA kit for Proteinase 3 (PR3) CLIA assay for Antigen Detection

Organism species: Pan-species (General)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins NONE Recombinant Proteinase 3 (PR3) Positive Control; Immunogen; SDS-PAGE; WB.
NONE Native Proteinase 3 (PR3) Bioactivity
Antibodies NONE Monoclonal Antibody to Proteinase 3 (PR3) WB; IHC; ICC; IP.
NONE Polyclonal Antibody to Proteinase 3 (PR3) WB; IHC; ICC; IF.
NONE Biotin Linked-Polyclonal Antibody to Proteinase 3 (PR3) ELISA(Det);IC; IH; IH(P); WB
NONE APC Linked-Polyclonal Antibody to Proteinase 3 (PR3) IHC, WB
NONE HRP Linked-Polyclonal Antibody to Proteinase 3 (PR3) Simple Western; WB
NONE PE Linked-Polyclonal Antibody to Proteinase 3 (PR3) Flow Cytometry
NONE FITC Linked-Polyclonal Antibody to Proteinase 3 (PR3) Flow Cytometry;ICC/IF
Assay Kits NONE ELISA Kit for Proteinase 3 (PR3) Enzyme-linked immunosorbent assay for Antigen Detection
NONE CLIA kit for Proteinase 3 (PR3) CLIA assay for Antigen Detection

Reference

"Wegener autoantigen and myeloblastin are encoded by a single mRNA."
Proc. Natl. Acad. Sci. U.S.A. 88:9253-9256(1991) [PubMed] [Europe PMC] [Abstract]

"The DNA sequence and biology of human chromosome 19."
Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Three human elastase-like genes coordinately expressed in the myelomonocyte lineage are organized as a single genetic locus on 19pter."
Proc. Natl. Acad. Sci. U.S.A. 89:8215-8219(1992) [PubMed] [Europe PMC] [Abstract]

"Structure, chromosomal assignment, and expression of the gene for proteinase-3. The Wegener's granulomatosis autoantigen."
J. Biol. Chem. 267:21193-21199(1992) [PubMed] [Europe PMC] [Abstract]

"Donor-recipient polymorphism of the proteinase 3 gene: a potential target for T-cell alloresponses to myeloid leukemia."
J. Immunother. 22:1-6(1999) [PubMed] [Europe PMC] [Abstract]

"Wegener's autoantigen and leukemia."
Blood 77:1398-1399(1991) [PubMed] [Europe PMC] [Abstract]

"Cloning of cDNA for proteinase 3: a serine protease, antibiotic, and autoantigen from human neutrophils."
J. Exp. Med. 172:1709-1715(1990) [PubMed] [Europe PMC] [Abstract]

"Down-regulation of a serine protease, myeloblastin, causes growth arrest and differentiation of promyelocytic leukemia cells."
Cell 59:959-968(1989) [PubMed] [Europe PMC] [Abstract]

"Wegener's autoantigen decoded."
Nature 346:520-520(1990) [PubMed] [Europe PMC] [Abstract]

"Characterization of proteinase-3 (PR-3), a neutrophil serine proteinase. Structural and functional properties."
J. Biol. Chem. 266:9540-9548(1991) [PubMed] [Europe PMC] [Abstract]

"Characterization of two azurphil granule proteases with active-site homology to neutrophil elastase."
J. Biol. Chem. 265:2038-2041(1990) [PubMed] [Europe PMC] [Abstract]

"Monoclonal antibodies specific for neutrophil proteinase 4. Production and use for isolation of the enzyme."
Biol. Chem. Hoppe-Seyler 371:549-555(1990) [PubMed] [Europe PMC] [Abstract]

"The relation of 29 kD C-ANCA antigen to proteinase 3."
APMIS Suppl. 19:26-27(1990) [PubMed] [Europe PMC] [Abstract]

"Wegener's granulomatosis autoantigen is a novel neutrophil serine proteinase."
Blood 74:1888-1893(1989) [PubMed] [Europe PMC] [Abstract]

"Antibiotic proteins of human polymorphonuclear leukocytes."
Proc. Natl. Acad. Sci. U.S.A. 86:5610-5614(1989) [PubMed] [Europe PMC] [Abstract]

"Use of proteinase 3 purified by reverse phase HPLC to detect autoantibodies in systemic vasculitis."
J. Immunol. Methods 180:25-33(1995) [PubMed] [Europe PMC] [Abstract]

"Anti-neutrophil cytoplasm antibodies in Wegener's granulomatosis recognize an elastinolytic enzyme."
J. Exp. Med. 171:357-362(1990) [PubMed] [Europe PMC] [Abstract]

"A novel form of dipeptidylpeptidase IV found in human serum. Isolation, characterization, and comparison with T lymphocyte membrane dipeptidylpeptidase IV (CD26)."
J. Biol. Chem. 270:14107-14114(1995) [PubMed] [Europe PMC] [Abstract]

"Identity of Wegener's autoantigen (p29) with proteinase 3 and myeloblastin."
Blood 76:2162-2162(1990) [PubMed] [Europe PMC] [Abstract]

"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

"The crystal structure of PR3, a neutrophil serine proteinase antigen of Wegener's granulomatosis antibodies."
J. Mol. Biol. 261:267-278(1996) [PubMed] [Europe PMC] [Abstract]