Call Now

S100 Calcium Binding Protein A8 (S100A8)

MRP8; MIF; 60B8AG; CAGA; CFAG; CGLA; CP-10; L1Ag; NIF; P8; Calgranulin A; Cystic fibrosis antigen; Leukocyte L1 complex light chain; Migration inhibitory factor-related protein 8

S100A8, a protein associated with a wide variety of inflammatory conditions, is heavily over-expressed in association with inflammation. Human S100A8 possesses neutrophil-repelling properties that result in an anti-inflammatory effect in vivo. The chemotactic activity of S100A8 on neutrophils was tested in Transwell chemotaxis assays. Analysis of the data indicates that S100A8 causes a repulsion of peripheral neutrophils, an activity that S100A8 loses upon its oxidation. S100A8 causes a strong anti-inflammatory effect in the rat air-pouch model of inflammation in vivo. These data highlight a naturally occurring novel anti-inflammatory pathway and provide potential molecular targets for the development of novel anti-inflammatory therapeutics.

Organism species: Homo sapiens (Human)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPV048Hu01 Recombinant S100 Calcium Binding Protein A8 (S100A8) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAV048Hu01 Monoclonal Antibody to S100 Calcium Binding Protein A8 (S100A8) WB; IHC; ICC; IP.
PAV048Hu01 Polyclonal Antibody to S100 Calcium Binding Protein A8 (S100A8) WB; IHC; ICC; IF.
Assay Kits CLV048Hu01 CLIA Kit for S100 Calcium Binding Protein A8 (S100A8) CLIA assay for Antigen Detection
ELV048Hu01 ELISA Kit for S100 Calcium Binding Protein A8 (S100A8) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Mus musculus (Mouse)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPV048Mu01 Recombinant S100 Calcium Binding Protein A8 (S100A8) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAV048Mu01 Monoclonal Antibody to S100 Calcium Binding Protein A8 (S100A8) WB; IHC; ICC; IP.
PAV048Mu01 Polyclonal Antibody to S100 Calcium Binding Protein A8 (S100A8) WB; IHC; ICC; IF.
Assay Kits CLV048Mu01 CLIA Kit for S100 Calcium Binding Protein A8 (S100A8) CLIA assay for Antigen Detection
ELV048Mu01 ELISA Kit for S100 Calcium Binding Protein A8 (S100A8) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Rattus norvegicus (Rat)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPV048Ra01 Recombinant S100 Calcium Binding Protein A8 (S100A8) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAV048Ra01 Monoclonal Antibody to S100 Calcium Binding Protein A8 (S100A8) WB; IHC; ICC; IP.
PAV048Ra01 Polyclonal Antibody to S100 Calcium Binding Protein A8 (S100A8) WB; IHC; ICC; IF.
Assay Kits CLV048Ra01 CLIA Kit for S100 Calcium Binding Protein A8 (S100A8) CLIA assay for Antigen Detection
ELV048Ra01 ELISA Kit for S100 Calcium Binding Protein A8 (S100A8) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Canis familiaris; Canine (Dog)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPV048Ca01 Recombinant S100 Calcium Binding Protein A8 (S100A8) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAV048Ca01 Monoclonal Antibody to S100 Calcium Binding Protein A8 (S100A8) WB; IHC; ICC; IP.
PAV048Ca01 Polyclonal Antibody to S100 Calcium Binding Protein A8 (S100A8) WB; IHC; ICC; IF.
Assay Kits CLV048Ca01 CLIA Kit for S100 Calcium Binding Protein A8 (S100A8) CLIA assay for Antigen Detection
ELV048Ca01 ELISA Kit for S100 Calcium Binding Protein A8 (S100A8) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Sus scrofa; Porcine (Pig)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPV048Po01 Recombinant S100 Calcium Binding Protein A8 (S100A8) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAV048Po01 Monoclonal Antibody to S100 Calcium Binding Protein A8 (S100A8) WB; IHC; ICC; IP.
PAV048Po01 Polyclonal Antibody to S100 Calcium Binding Protein A8 (S100A8) WB; IHC; ICC; IF.
Assay Kits CLV048Po01 CLIA Kit for S100 Calcium Binding Protein A8 (S100A8) CLIA assay for Antigen Detection
ELV048Po01 ELISA Kit for S100 Calcium Binding Protein A8 (S100A8) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Bos taurus; Bovine (Cattle)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPV048Bo01 Recombinant S100 Calcium Binding Protein A8 (S100A8) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAV048Bo01 Monoclonal Antibody to S100 Calcium Binding Protein A8 (S100A8) WB; IHC; ICC; IP.
PAV048Bo01 Polyclonal Antibody to S100 Calcium Binding Protein A8 (S100A8) WB; IHC; ICC; IF.
Assay Kits CLV048Bo01 CLIA Kit for S100 Calcium Binding Protein A8 (S100A8) CLIA assay for Antigen Detection
ELV048Bo01 ELISA Kit for S100 Calcium Binding Protein A8 (S100A8) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Oryctolagus cuniculus (Rabbit)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPV048Rb01 Recombinant S100 Calcium Binding Protein A8 (S100A8) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAV048Rb01 Monoclonal Antibody to S100 Calcium Binding Protein A8 (S100A8) WB; IHC; ICC; IP.
PAV048Rb01 Polyclonal Antibody to S100 Calcium Binding Protein A8 (S100A8) WB; IHC; ICC; IF.
Assay Kits CLV048Rb01 CLIA Kit for S100 Calcium Binding Protein A8 (S100A8) CLIA assay for Antigen Detection
ELV048Rb01 ELISA Kit for S100 Calcium Binding Protein A8 (S100A8) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Rhesus monkey (Simian)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPV048Mo01 Recombinant S100 Calcium Binding Protein A8 (S100A8) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAV048Mo01 Monoclonal Antibody to S100 Calcium Binding Protein A8 (S100A8) WB; IHC; ICC; IP.
PAV048Mo01 Polyclonal Antibody to S100 Calcium Binding Protein A8 (S100A8) WB; IHC; ICC; IF.
Assay Kits CLV048Mo01 CLIA Kit for S100 Calcium Binding Protein A8 (S100A8) CLIA assay for Antigen Detection
ELV048Mo01 ELISA Kit for S100 Calcium Binding Protein A8 (S100A8) Enzyme-linked immunosorbent assay for Antigen Detection

Reference

"A clue to the basic defect in cystic fibrosis from cloning the CF antigen gene."
Nature 326:614-617(1987) [PubMed] [Europe PMC] [Abstract]

"Two calcium-binding proteins in infiltrate macrophages of rheumatoid arthritis."
Nature 330:80-82(1987) [PubMed] [Europe PMC] [Abstract]

"Cloning and expression of two human genes encoding calcium-binding proteins that are regulated during myeloid differentiation."
Mol. Cell. Biol. 8:2402-2410(1988) [PubMed] [Europe PMC] [Abstract]

"The calcium-binding protein MRP-8 is produced by human pulmonary tumor cells."
Biol. Chem. Hoppe-Seyler 372:1-4(1991) [PubMed] [Europe PMC] [Abstract]

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

"The DNA sequence and biological annotation of human chromosome 1."
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Host-parasite interaction in human onchocerciasis: identification and sequence analysis of a novel human calgranulin."
Biochem. Biophys. Res. Commun. 221:454-458(1996) [PubMed] [Europe PMC] [Abstract]

"In vitro antimicrobial activity of the human neutrophil cytosolic S-100 protein complex, calprotectin, against Capnocytophaga sputigena."
J. Dent. Res. 72:517-523(1993) [PubMed] [Europe PMC] [Abstract]

"Translocation of a small cytosolic calcium-binding protein (MRP-8) to plasma membrane correlates with human neutrophil activation."
J. Biol. Chem. 267:19379-19382(1992) [PubMed] [Europe PMC] [Abstract]

"Calprotectin-like protein is related to soluble organic matrix in calcium oxalate urinary stone."
Biochem. Mol. Biol. Int. 34:309-313(1994) [PubMed] [Europe PMC] [Abstract]

"Isolation of an ascitic oncodevelopmental protein exhibiting high sequence homology with calcium-binding protein MRP8."
Biol. Chem. Hoppe-Seyler 375:789-792(1994) [PubMed] [Europe PMC] [Abstract]

"Microsequences of 145 proteins recorded in the two-dimensional gel protein database of normal human epidermal keratinocytes."
Electrophoresis 13:960-969(1992) [PubMed] [Europe PMC] [Abstract]

"Myeloid-related protein (MRP) 8 and MRP14, calcium-binding proteins of the S100 family, are secreted by activated monocytes via a novel, tubulin-dependent pathway."
J. Biol. Chem. 272:9496-9502(1997) [PubMed] [Europe PMC] [Abstract]

"The microbial receptor CEACAM3 is linked to the calprotectin complex in granulocytes."
Biochem. Biophys. Res. Commun. 289:191-197(2001) [PubMed] [Europe PMC] [Abstract]

"Proinflammatory activities of S100: proteins S100A8, S100A9, and S100A8/A9 induce neutrophil chemotaxis and adhesion."
J. Immunol. 170:3233-3242(2003) [PubMed] [Europe PMC] [Abstract]

"MRP8 and MRP14 control microtubule reorganization during transendothelial migration of phagocytes."
Blood 104:4260-4268(2004) [PubMed] [Europe PMC] [Abstract]

"Myeloid-related proteins 8 and 14 induce a specific inflammatory response in human microvascular endothelial cells."
Blood 105:2955-2962(2005) [PubMed] [Europe PMC] [Abstract]

"The arachidonic acid-binding protein S100A8/A9 promotes NADPH oxidase activation by interaction with p67phox and Rac-2."
FASEB J. 19:467-469(2005) [PubMed] [Europe PMC] [Abstract]

"Regulation of S100A8/A9 (calprotectin) binding to tumor cells by zinc ion and its implication for apoptosis-inducing activity."
Mediators Inflamm. 2005:280-292(2005) [PubMed] [Europe PMC] [Abstract]

"Interaction between S100A8/A9 and annexin A6 is involved in the calcium-induced cell surface exposition of S100A8/A9."
J. Biol. Chem. 283:31776-31784(2008) [PubMed] [Europe PMC] [Abstract]

"S-nitrosylated S100A8: novel anti-inflammatory properties."
J. Immunol. 181:5627-5636(2008) [PubMed] [Europe PMC] [Abstract]

"Anti-infective protective properties of S100 calgranulins."
Antiinflamm. Antiallergy Agents Med. Chem. 8:290-305(2009) [PubMed] [Europe PMC] [Abstract]

"S100A8/A9: a Janus-faced molecule in cancer therapy and tumorgenesis."
Eur. J. Pharmacol. 625:73-83(2009) [PubMed] [Europe PMC] [Abstract]

"Substitution of methionine 63 or 83 in S100A9 and cysteine 42 in S100A8 abrogate the antifungal activities of S100A8/A9: potential role for oxidative regulation."
FEMS Immunol. Med. Microbiol. 55:55-61(2009) [PubMed] [Europe PMC] [Abstract]

"Calprotectin S100A9 calcium-binding loops I and II are essential for keratinocyte resistance to bacterial invasion."
J. Biol. Chem. 284:7078-7090(2009) [PubMed] [Europe PMC] [Abstract]

"The endogenous Toll-like receptor 4 agonist S100A8/S100A9 (calprotectin) as innate amplifier of infection, autoimmunity, and cancer."
J. Leukoc. Biol. 86:557-566(2009) [PubMed] [Europe PMC] [Abstract]

"S100A8/A9 induces autophagy and apoptosis via ROS-mediated cross-talk between mitochondria and lysosomes that involves BNIP3."
Cell Res. 20:314-331(2010) [PubMed] [Europe PMC] [Abstract]

"S100 Calgranulins in inflammatory arthritis."
Immunol. Cell Biol. 88:41-49(2010) [PubMed] [Europe PMC] [Abstract]

"Inflammation-associated S100 proteins: new mechanisms that regulate function."
Amino Acids 41:821-842(2011) [PubMed] [Europe PMC] [Abstract]

"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

"S100A8 and S100A9 in cardiovascular biology and disease."
Arterioscler. Thromb. Vasc. Biol. 32:223-229(2012) [PubMed] [Europe PMC] [Abstract]

"Dynamic mobility of immunological cells expressing S100A8 and S100A9 in vivo: a variety of functional roles of the two proteins as regulators in acute inflammatory reaction."
Inflammation 35:409-419(2012) [PubMed] [Europe PMC] [Abstract]

"Pro-inflammatory S100A8 and S100A9 proteins: self-assembly into multifunctional native and amyloid complexes."
Int. J. Mol. Sci. 13:2893-2917(2012) [PubMed] [Europe PMC] [Abstract]

"S100A8 and S100A9: new insights into their roles in malignancy."
J. Innate Immun. 4:31-40(2012) [PubMed] [Europe PMC] [Abstract]

"Molecular interface of S100A8 with cytochrome b and NADPH oxidase activation."
PLoS ONE 7:E40277-E40277(2012) [PubMed] [Europe PMC] [Abstract]

"Constitutive neutrophil apoptosis: regulation by cell concentration via S100 A8/9 and the MEK-ERK pathway."
PLoS ONE 7:E29333-E29333(2012) [PubMed] [Europe PMC] [Abstract]

"Target-selective protein S-nitrosylation by sequence motif recognition."
Cell 159:623-634(2014) [PubMed] [Europe PMC] [Abstract]

"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]

"The structure of human MRP8, a member of the S100 calcium-binding protein family, by MAD phasing at 1.9 A resolution."
Acta Crystallogr. D 56:559-566(2000) [PubMed] [Europe PMC] [Abstract]

"The crystal structure of the human (S100A8/S100A9)2 heterotetramer, calprotectin, illustrates how conformational changes of interacting alpha-helices can determine specific association of two EF-hand proteins."
J. Mol. Biol. 370:887-898(2007) [PubMed] [Europe PMC] [Abstract]