Paxillin (Pax)

PXN

SPN	U996
HGNC	9718
MGI	108295
PubMed	11146675
UniProt	P49023
Wiki	Paxillin

Paxillin is a signal transduction adaptor protein and should not be confused with the neurotoxin paxilline.Glenney and Zokas (1989) used an antiphosphotyrosine antibody to identify proteins that are phosphorylated in Rous sarcoma virus-transformed chick embryo fibroblasts, and found a 76-kD protein that localizes to focal adhesions at the ends of actin-containing stress fibers in nontransformed cells. Turner et al. (1990) purified this protein from chicken gizzard smooth muscle, and named it paxillin ('paxillus' means 'small stake' or 'peg' in Latin) as a protein tethered to the membrane at focal adhesions.Paxillin migrates as a diffuse 65- to 70-kD band on SDS-PAGE. Salgia et al. (1995) stated that transmembrane integrin molecules connect the actin cytoskeleton to the extracellular matrix within focal adhesions.

Reference

"Molecular cloning of human paxillin, a focal adhesion protein phosphorylated by P210BCR/ABL."
J. Biol. Chem. 270:5039-5047(1995) [PubMed] [Europe PMC] [Abstract]

"Monocyte cells and cancer cells express novel paxillin isoforms with different binding properties to focal adhesion proteins."
J. Biol. Chem. 272:7437-7444(1997) [PubMed] [Europe PMC] [Abstract]

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

"The full-ORF clone resource of the German cDNA consortium."
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]

"The finished DNA sequence of human chromosome 12."
Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Binding of paxillin to alpha4 integrins modifies integrin-dependent biological responses."
Nature 402:676-681(1999) [PubMed] [Europe PMC] [Abstract]

"Coupling of PAK-interacting exchange factor PIX to GIT1 promotes focal complex disassembly."
Mol. Cell. Biol. 20:6354-6363(2000) [PubMed] [Europe PMC] [Abstract]

"A new paxillin-binding protein, PAG3/Papalpha/KIAA0400, bearing an ADP-ribosylation factor GTPase-activating protein activity, is involved in paxillin recruitment to focal adhesions and cell migration."
Mol. Biol. Cell 11:1315-1327(2000) [PubMed] [Europe PMC] [Abstract]

"Involvement of phosphorylation of Tyr-31 and Tyr-118 of paxillin in MM1 cancer cell migration."
Int. J. Cancer 97:330-335(2002) [PubMed] [Europe PMC] [Abstract]

"RNF5, a RING finger protein that regulates cell motility by targeting paxillin ubiquitination and altered localization."
Mol. Cell. Biol. 23:5331-5345(2003) [PubMed] [Europe PMC] [Abstract]

"Brk activates rac1 and promotes cell migration and invasion by phosphorylating paxillin."
Mol. Cell. Biol. 24:10558-10572(2004) [PubMed] [Europe PMC] [Abstract]

"Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]

"A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]

"Cdk5 regulates differentiation of oligodendrocyte precursor cells through the direct phosphorylation of paxillin."
J. Cell Sci. 120:4355-4366(2007) [PubMed] [Europe PMC] [Abstract]

"Nek3 kinase regulates prolactin-mediated cytoskeletal reorganization and motility of breast cancer cells."
Oncogene 26:4668-4678(2007) [PubMed] [Europe PMC] [Abstract]

"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]

"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]

"A quantitative atlas of mitotic phosphorylation."
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]

"Large-scale proteomics analysis of the human kinome."
Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]

"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]

"Crystal structure of CCM3, a cerebral cavernous malformation protein critical for vascular integrity."
J. Biol. Chem. 285:24099-24107(2010) [PubMed] [Europe PMC] [Abstract]

"CD36 ligands promote sterile inflammation through assembly of a Toll-like receptor 4 and 6 heterodimer."
Nat. Immunol. 11:155-161(2010) [PubMed] [Europe PMC] [Abstract]

"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]

"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]

"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]

"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]

"Molecular recognition of paxillin LD motifs by the focal adhesion targeting domain."
Structure 11:1207-1217(2003) [PubMed] [Europe PMC] [Abstract]

"Paxillin and ponsin interact in nascent costameres of muscle cells."
J. Mol. Biol. 369:665-682(2007) [PubMed] [Europe PMC] [Abstract]

"The structure of alpha-parvin CH2-paxillin LD1 complex reveals a novel modular recognition for focal adhesion assembly."
J. Biol. Chem. 283:21113-21119(2008) [PubMed] [Europe PMC] [Abstract]

"Structural analysis of the interactions between paxillin LD motifs and alpha-parvin."
Structure 16:1521-1531(2008) [PubMed] [Europe PMC] [Abstract]

"Crystal structures of free and ligand-bound focal adhesion targeting domain of Pyk2."
Biochem. Biophys. Res. Commun. 383:347-352(2009) [PubMed] [Europe PMC] [Abstract]

"Structural basis for paxillin binding and focal adhesion targeting of beta-parvin."
J. Biol. Chem. 287:32566-32577(2012) [PubMed] [Europe PMC] [Abstract]