Complement Component 5 (C5)

CPAMD4; C5a anaphylatoxin; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4

SPN	U873
HGNC	1331
MGI	96031
PubMed	23806181
RGD	2237
UniProt	P01031
Wiki	C5

Complement component 5 is a protein involved in the complement system.C5 is a 190-kD glycoprotein comprised of 2 disulfide-linked polypeptide chains, alpha (C5a) and beta (C5b), with a molecular mass of 155 and 75 kD, respectively (Tack et al., 1979). Haviland et al. (1991) constructed the complete cDNA sequence of human complement pro-C5, which is predicted to encode a 1,676-amino acid promolecule that contains an 18-amino acid leader peptide and a 4-amino acid linker separating the beta and alpha chains.

Northern blot analysis demonstrated a major 6.0-kb C5 transcript, as well as 3.0-kb and 4.8-kb transcripts. Carney et al. (1991) determined that the C5 gene contains 41 exons that span a genomic region of 79 kb.

Reference

"Complete cDNA sequence of human complement pro-C5. Evidence of truncated transcripts derived from a single copy gene."
J. Immunol. 146:362-368(1991) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Molecular analysis of human complement component C5: localization of the structural gene to chromosome 9."
Biochemistry 27:1474-1482(1988) [PubMed] [Europe PMC] [Abstract]

"Isolation and sequence analysis of a cDNA clone encoding the fifth complement component."
J. Biol. Chem. 260:2108-2112(1985) [PubMed] [Europe PMC] [Abstract]

"Primary structural analysis of the polypeptide portion of human C5a anaphylatoxin. Polypeptide sequence determination and assignment of the oligosaccharide attachment site in C5a."
J. Biol. Chem. 253:6955-6964(1978) [PubMed] [Europe PMC] [Abstract]

"Group B streptococci inactivate complement component C5a by enzymic cleavage at the C-terminus."
Biochem. J. 273:635-640(1991) [PubMed] [Europe PMC] [Abstract]

"The amino terminus of the human C5a receptor is required for high affinity C5a binding and for receptor activation by C5a but not C5a analogs."
J. Biol. Chem. 269:14446-14450(1994) [PubMed] [Europe PMC] [Abstract]

"Residues 21-30 within the extracellular N-terminal region of the C5a receptor represent a binding domain for the C5a anaphylatoxin."
J. Biol. Chem. 273:10411-10419(1998) [PubMed] [Europe PMC] [Abstract]

"Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry."
J. Proteome Res. 4:2070-2080(2005) [PubMed] [Europe PMC] [Abstract]

"Sequence-specific assignments in the 1H NMR spectrum of the human inflammatory protein C5a."
Biochemistry 27:3568-3580(1988) [PubMed] [Europe PMC] [Abstract]

"Tertiary structure of human complement component C5a in solution from nuclear magnetic resonance data."
Biochemistry 28:172-185(1989) [PubMed] [Europe PMC] [Abstract]

"Heteronuclear three-dimensional NMR spectroscopy of the inflammatory protein C5a."
Biochemistry 28:2387-2391(1989) [PubMed] [Europe PMC] [Abstract]

"Solution structure of a unique C5a semi-synthetic antagonist: implications in receptor binding."
Protein Sci. 6:65-72(1997) [PubMed] [Europe PMC] [Abstract]

"Structural definition of the C5a C-terminus by two-dimensional nuclear magnetic resonance spectroscopy."
Proteins 28:261-267(1997) [PubMed] [Europe PMC] [Abstract]

"Inherited human complement C5 deficiency. Nonsense mutations in exons 1 (Gln1 to Stop) and 36 (Arg1458 to Stop) and compound heterozygosity in three African-American families."
J. Immunol. 154:5464-5471(1995) [PubMed] [Europe PMC] [Abstract]

"Linking C5 deficiency to an exonic splicing enhancer mutation."
J. Immunol. 174:4172-4177(2005) [PubMed] [Europe PMC] [Abstract]

"C5 complement deficiency in a Spanish family. Molecular characterization of the double mutation responsible for the defect."
Mol. Immunol. 42:105-111(2005) [PubMed] [Europe PMC] [Abstract]

"Complement factor 5 is a quantitative trait gene that modifies liver fibrogenesis in mice and humans."
Nat. Genet. 37:835-843(2005) [PubMed] [Europe PMC] [Abstract]

"Functional insights from the structure of the multifunctional C345C domain of C5 of complement."
J. Biol. Chem. 280:10636-10645(2005) [PubMed] [Europe PMC] [Abstract]

"Structure of and influence of a tick complement inhibitor on human complement component 5."
Nat. Immunol. 9:753-760(2008) [PubMed] [Europe PMC] [Abstract]

"Substrate recognition by complement convertases revealed in the C5-cobra venom factor complex."
EMBO J. 30:606-616(2011) [PubMed] [Europe PMC] [Abstract]

"Quantitative detection of single amino acid polymorphisms by targeted proteomics."
J. Mol. Cell Biol. 3:309-315(2011) [PubMed] [Europe PMC] [Abstract]

"Genetic variants in C5 and poor response to eculizumab."
N. Engl. J. Med. 370:632-639(2014) [PubMed] [Europe PMC] [Abstract]