Protein Kinase N2 (PKN2)

PRK2; Pak-2; PRKCL2; Protein Kinase C-Like 2; Protein-kinase C-related kinase 2; Serine/threonine-protein kinase N2; Cardiolipin-Activated Protein Kinase Pak2

SPN	U856
HGNC	9406
MGI	109211
PubMed	8932326
RGD	620146
UniProt	Q16513
Wiki	PKN2

The cDNA sequence predicts a 984-amino acid polypeptide. Expression of this cDNA in COS cells produced a 130-kD protein product that was capable of autophosphorylation, indicating intrinsic kinase activity. Using Northern blot and RT-PCR analyses to examine expression patterns in a wide variety of tissues, Palmer et al. (1995) detected PRK2 expression in DX3 B-cell myeloma cells only. Yu et al. (1997) purified a protein kinase, termed PAK2, from rat liver and showed that the corresponding gene is the rat homolog of human PRK2. PAK2 has biochemical properties similar to, but distinct from PRK1/PAK1. PAK2 is activated by lipids, particularly cardiolipin.The International Radiation Hybrid Mapping Consortium mapped the PKN2 gene to chromosome 1

Reference

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"The PRK2 kinase is a potential effector target of both Rho and Rac GTPases and regulates actin cytoskeletal organization."
Mol. Cell. Biol. 17:2247-2256(1997) [PubMed] [Europe PMC] [Abstract]

"PDK1 acquires PDK2 activity in the presence of a synthetic peptide derived from the carboxyl terminus of PRK2."
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"The protein kinase C-related kinase PRK2 interacts with the protein tyrosine phosphatase PTP-BL via a novel PDZ domain binding motif."
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"Rho GTPases regulate PRK2/PKN2 to control entry into mitosis and exit from cytokinesis."
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"Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra."
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"The C-terminus of PRK2/PKNgamma is required for optimal activation by RhoA in a GTP-dependent manner."
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"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
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"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]

"Protein kinase C-related kinase targets nuclear localization signals in a subset of class IIa histone deacetylases."
FEBS Lett. 584:1103-1110(2010) [PubMed] [Europe PMC] [Abstract]

"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
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"Initial characterization of the human central proteome."
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"The Rho target PRK2 regulates apical junction formation in human bronchial epithelial cells."
Mol. Cell. Biol. 31:81-91(2011) [PubMed] [Europe PMC] [Abstract]

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"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]