Large Neutral Amino Acid Transporter 1 (LAT1)

CD98; MDU1; 4T2HC; 4F2; NACAE; SLC7A5; MPE16; Solute Carrier Family 7 Member 5; Cationic Amino Acid Transporter,y+ System; L-Type Amino Acid Transporter

SPN	U838
HGNC	11063
MGI	1298205
PubMed	11901210
RGD	620639
UniProt	Q01650
Wiki	CD98

LAT1 is a heterodimeric membrane transport protein that preferentially transports neutral branched (valine, leucine, isoleucine) and aromatic (tryptophan, tyrosine) amino acids. LAT is highly expressed in brain capillaries (which form the blood brain barrier) relative to other tissues.CD98 is a glycoprotein that comprises the light subunit of the Large neutral Amino acid Transporter (LAT1).

Kanai et al. (1998) used expression cloning to isolate a rat cDNA termed LAT1. They showed that LAT1 encoded a protein necessary for system L amino acid transport, thought to be a major route by which cells import large neutral amino acids with branched or aromatic side chains.

Reference

"Amino-acid transport by heterodimers of 4F2hc/CD98 and members of a permease family."
Nature 395:288-291(1998) [PubMed] [Europe PMC] [Abstract]

"Human LAT1, a subunit of system L amino acid transporter: molecular cloning and transport function."
Biochem. Biophys. Res. Commun. 255:283-288(1999) [PubMed] [Europe PMC] [Abstract]

"Primary structure of the light chain of fusion regulatory protein-1/CD98/4F2 predicts a protein with multiple transmembrane domains that is almost identical to the amino acid transporter E16."
J. Immunol. 162:2462-2466(1999) [PubMed] [Europe PMC] [Abstract]

"Human L-type amino acid transporter 1 (LAT1): characterization of function and expression in tumor cell lines."
Biochim. Biophys. Acta 1514:291-302(2001) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"A novel transiently expressed, integral membrane protein linked to cell activation. Molecular cloning via the rapid degradation signal AUUUA."
J. Biol. Chem. 267:11267-11273(1992) [PubMed] [Europe PMC] [Abstract]

"Identification of a membrane protein, LAT-2, that co-expresses with 4F2 heavy chain, an L-type amino acid transport activity with broad specificity for small and large zwitterionic amino acids."
J. Biol. Chem. 274:19738-19744(1999) [PubMed] [Europe PMC] [Abstract]

"LAT2, a new basolateral 4F2hc/CD98-associated amino acid transporter of kidney and intestine."
J. Biol. Chem. 274:34948-34954(1999) [PubMed] [Europe PMC] [Abstract]

"Association of 4F2hc with light chains LAT1, LAT2 or y+LAT2 requires different domains."
Biochem. J. 355:725-731(2001) [PubMed] [Europe PMC] [Abstract]

"Role of the System L permease LAT1 in amino acid and iodothyronine transport in placenta."
Biochem. J. 356:719-725(2001) [PubMed] [Europe PMC] [Abstract]

"Thyroid hormone transport by the heterodimeric human system L amino acid transporter."
Endocrinology 142:4339-4348(2001) [PubMed] [Europe PMC] [Abstract]

"Expression and regulation of 4F2hc and hLAT1 in human trophoblasts."
Am. J. Physiol. 282:C196-C204(2002) [PubMed] [Europe PMC] [Abstract]

"Transport of a neurotoxicant by molecular mimicry: the methylmercury-L-cysteine complex is a substrate for human L-type large neutral amino acid transporter (LAT) 1 and LAT2."
Biochem. J. 367:239-246(2002) [PubMed] [Europe PMC] [Abstract]

"Characterization of the system L amino acid transporter in T24 human bladder carcinoma cells."
Biochim. Biophys. Acta 1565:112-121(2002) [PubMed] [Europe PMC] [Abstract]

"Identification and functional characterization of a Na+-independent large neutral amino acid transporter, LAT1, in human and rabbit cornea."
Invest. Ophthalmol. Vis. Sci. 44:2919-2927(2003) [PubMed] [Europe PMC] [Abstract]

"Expression of LAT1 and LAT2 amino acid transporters in human and rat intestinal epithelial cells."
Amino Acids 29:229-233(2005) [PubMed] [Europe PMC] [Abstract]

"Identification of stereoselective transporters for S-nitroso-L-cysteine: role of LAT1 and LAT2 in biological activity of S-nitrosothiols."
J. Biol. Chem. 280:20102-20110(2005) [PubMed] [Europe PMC] [Abstract]

"L-type amino acid transporter 1 as a potential molecular target in human astrocytic tumors."
Int. J. Cancer 119:484-492(2006) [PubMed] [Europe PMC] [Abstract]

"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]

"Functional characterization of tyrosine transport in fibroblast cells from healthy controls."
Neurosci. Lett. 434:56-60(2008) [PubMed] [Europe PMC] [Abstract]

"A quantitative atlas of mitotic phosphorylation."
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

"Large-scale proteomics analysis of the human kinome."
Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]

"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]

"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]