Dihydropyrimidine Dehydrogenase (DPYD)

DPD; DHPDHase; Dihydrothymine dehydrogenase; Dihydrouracil dehydrogenase

SPN	U831
HGNC	3012
MGI	2139667
PubMed	20920994
RGD	621218
UniProt	Q12882
Wiki	DPYD

Dihydropyrimidine dehydrogenase (DPD) is an enzyme that is involved in pyrimidine degradation. It is the initial and rate-limiting step in pyrimidine catabolism. It catalyzes the reduction of uracil and thymine. It is also involved in the degradation of the chemotherapeutic drugs 5-fluorouracil and Tegafur-uracil.The sequence of the gene suggested that DPD has at least 3 distinct domains: a possible NADPH binding site and FAD-binding site in the N terminus, 2 motifs of putative iron/sulfur-binding sites near the C terminus, and a peptide domain corresponding to the uracil-binding site.

Expression of the pig enzyme in E. coli catalyzed the reduction of uracil, thymine, and 5-fluorouracil (5FU) with kinetics approximating those published for the enzyme purified from mammalian liver.

Reference

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