17-Alpha-Hydroxylase (S17aH)
CYP17A1; CPT7; P450C17; 17-alpha-hydroxyprogesterone aldolase/17,20-Lyase; Steroid 17-alpha-monooxygenase; Cytochrome P450,Family 17,Subfamily A,Polypeptide 1
CYP17A1 (also known as 17 alpha-hydroxylase/17,20 lyase/17,20 desmolase) is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids.
This protein localizes to the endoplasmic reticulum. It has both 17alpha-hydroxylase and 17,20-lyase activities and is a key enzyme in the steroidogenic pathway that produces progestins, mineralocorticoids, glucocorticoids, androgens, and estrogens. Mutations in this gene are associated with isolated steroid-17 alpha-hydroxylase deficiency, 17-alpha-hydroxylase/17,20-lyase deficiency, pseudohermaphroditism, and adrenal hyperplasia.
Organism species: Homo sapiens (Human)
PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
---|---|---|---|
Proteins | RPU827Hu01 | Recombinant 17-Alpha-Hydroxylase (S17aH) | Positive Control; Immunogen; SDS-PAGE; WB. |
Antibodies | MAU827Hu01 | Monoclonal Antibody to 17-Alpha-Hydroxylase (S17aH) | WB; IHC; ICC; IP. |
PAU827Hu01 | Polyclonal Antibody to 17-Alpha-Hydroxylase (S17aH) | WB; IHC; ICC; IF. | |
Assay Kits | CLU827Hu01 | CLIA Kit for 17-Alpha-Hydroxylase (S17aH) | CLIA assay for Antigen Detection |
ELU827Hu01 | ELISA Kit for 17-Alpha-Hydroxylase (S17aH) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Mus musculus (Mouse)
PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
---|---|---|---|
Proteins | RPU827Mu01 | Recombinant 17-Alpha-Hydroxylase (S17aH) | Positive Control; Immunogen; SDS-PAGE; WB. |
Antibodies | MAU827Mu01 | Monoclonal Antibody to 17-Alpha-Hydroxylase (S17aH) | WB; IHC; ICC; IP. |
PAU827Mu01 | Polyclonal Antibody to 17-Alpha-Hydroxylase (S17aH) | WB; IHC; ICC; IF. | |
Assay Kits | CLU827Mu01 | CLIA Kit for 17-Alpha-Hydroxylase (S17aH) | CLIA assay for Antigen Detection |
ELU827Mu01 | ELISA Kit for 17-Alpha-Hydroxylase (S17aH) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Rattus norvegicus (Rat)
PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
---|---|---|---|
Proteins | RPU827Ra01 | Recombinant 17-Alpha-Hydroxylase (S17aH) | Positive Control; Immunogen; SDS-PAGE; WB. |
Antibodies | MAU827Ra01 | Monoclonal Antibody to 17-Alpha-Hydroxylase (S17aH) | WB; IHC; ICC; IP. |
PAU827Ra01 | Polyclonal Antibody to 17-Alpha-Hydroxylase (S17aH) | WB; IHC; ICC; IF. | |
Assay Kits | CLU827Ra01 | CLIA Kit for 17-Alpha-Hydroxylase (S17aH) | CLIA assay for Antigen Detection |
ELU827Ra01 | ELISA Kit for 17-Alpha-Hydroxylase (S17aH) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Canis familiaris; Canine (Dog)
PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
---|---|---|---|
Proteins | RPU827Ca01 | Recombinant 17-Alpha-Hydroxylase (S17aH) | Positive Control; Immunogen; SDS-PAGE; WB. |
Antibodies | MAU827Ca01 | Monoclonal Antibody to 17-Alpha-Hydroxylase (S17aH) | WB; IHC; ICC; IP. |
PAU827Ca01 | Polyclonal Antibody to 17-Alpha-Hydroxylase (S17aH) | WB; IHC; ICC; IF. | |
Assay Kits | CLU827Ca01 | CLIA Kit for 17-Alpha-Hydroxylase (S17aH) | CLIA assay for Antigen Detection |
ELU827Ca01 | ELISA Kit for 17-Alpha-Hydroxylase (S17aH) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Sus scrofa; Porcine (Pig)
PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
---|---|---|---|
Proteins | RPU827Po01 | Recombinant 17-Alpha-Hydroxylase (S17aH) | Positive Control; Immunogen; SDS-PAGE; WB. |
Antibodies | MAU827Po01 | Monoclonal Antibody to 17-Alpha-Hydroxylase (S17aH) | WB; IHC; ICC; IP. |
PAU827Po01 | Polyclonal Antibody to 17-Alpha-Hydroxylase (S17aH) | WB; IHC; ICC; IF. | |
Assay Kits | CLU827Po01 | CLIA Kit for 17-Alpha-Hydroxylase (S17aH) | CLIA assay for Antigen Detection |
ELU827Po01 | ELISA Kit for 17-Alpha-Hydroxylase (S17aH) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Bos taurus; Bovine (Cattle)
PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
---|---|---|---|
Proteins | RPU827Bo01 | Recombinant 17-Alpha-Hydroxylase (S17aH) | Positive Control; Immunogen; SDS-PAGE; WB. |
Antibodies | MAU827Bo01 | Monoclonal Antibody to 17-Alpha-Hydroxylase (S17aH) | WB; IHC; ICC; IP. |
PAU827Bo01 | Polyclonal Antibody to 17-Alpha-Hydroxylase (S17aH) | WB; IHC; ICC; IF. | |
Assay Kits | CLU827Bo01 | CLIA Kit for 17-Alpha-Hydroxylase (S17aH) | CLIA assay for Antigen Detection |
ELU827Bo01 | ELISA Kit for 17-Alpha-Hydroxylase (S17aH) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Oryctolagus cuniculus (Rabbit)
PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
---|---|---|---|
Proteins | RPU827Rb01 | Recombinant 17-Alpha-Hydroxylase (S17aH) | Positive Control; Immunogen; SDS-PAGE; WB. |
Antibodies | MAU827Rb01 | Monoclonal Antibody to 17-Alpha-Hydroxylase (S17aH) | WB; IHC; ICC; IP. |
PAU827Rb01 | Polyclonal Antibody to 17-Alpha-Hydroxylase (S17aH) | WB; IHC; ICC; IF. | |
Assay Kits | CLU827Rb01 | CLIA Kit for 17-Alpha-Hydroxylase (S17aH) | CLIA assay for Antigen Detection |
ELU827Rb01 | ELISA Kit for 17-Alpha-Hydroxylase (S17aH) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Rhesus monkey (Simian)
PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
---|---|---|---|
Proteins | RPU827Mo01 | Recombinant 17-Alpha-Hydroxylase (S17aH) | Positive Control; Immunogen; SDS-PAGE; WB. |
Antibodies | MAU827Mo01 | Monoclonal Antibody to 17-Alpha-Hydroxylase (S17aH) | WB; IHC; ICC; IP. |
PAU827Mo01 | Polyclonal Antibody to 17-Alpha-Hydroxylase (S17aH) | WB; IHC; ICC; IF. | |
Assay Kits | CLU827Mo01 | CLIA Kit for 17-Alpha-Hydroxylase (S17aH) | CLIA assay for Antigen Detection |
ELU827Mo01 | ELISA Kit for 17-Alpha-Hydroxylase (S17aH) | Enzyme-linked immunosorbent assay for Antigen Detection |
Reference
"Cytochrome P450c17 (steroid 17 alpha-hydroxylase/17,20 lyase): cloning of human adrenal and testis cDNAs indicates the same gene is expressed in both tissues."
Proc. Natl. Acad. Sci. U.S.A. 84:407-411(1987) [PubMed] [Europe PMC] [Abstract]
"Cloning and sequence of the human gene for P450c17 (steroid 17 alpha-hydroxylase/17,20 lyase): similarity with the gene for P450c21."
DNA 6:439-448(1987) [PubMed] [Europe PMC] [Abstract]
"Characterization of complementary deoxyribonucleic acid for human adrenocortical 17 alpha-hydroxylase: a probe for analysis of 17 alpha-hydroxylase deficiency."
Mol. Endocrinol. 1:348-354(1987) [PubMed] [Europe PMC] [Abstract]
"Tissue-specific, cyclic adenosine 3',5'-monophosphate-induced, and phorbol ester-repressed transcription from the human P450c17 promoter in mouse cells."
Mol. Endocrinol. 4:1972-1979(1990) [PubMed] [Europe PMC] [Abstract]
"Structural characterization of normal and mutant human steroid 17 alpha-hydroxylase genes: molecular basis of one example of combined 17 alpha-hydroxylase/17,20 lyase deficiency."
Mol. Endocrinol. 2:564-570(1988) [PubMed] [Europe PMC] [Abstract]
"The DNA sequence and comparative analysis of human chromosome 10."
Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
"Molecular modeling of human P450c17 (17alpha-hydroxylase/17,20-lyase): insights into reaction mechanisms and effects of mutations."
Mol. Endocrinol. 13:1169-1182(1999) [PubMed] [Europe PMC] [Abstract]
"Structures of cytochrome P450 17A1 with prostate cancer drugs abiraterone and TOK-001."
Nature 482:116-119(2012) [PubMed] [Europe PMC] [Abstract]
"Deletion of a phenylalanine in the N-terminal region of human cytochrome P-450(17 alpha) results in partial combined 17 alpha-hydroxylase/17,20-lyase deficiency."
J. Biol. Chem. 264:18076-18082(1989) [PubMed] [Europe PMC] [Abstract]
"Missense mutation serine106-->proline causes 17 alpha-hydroxylase deficiency."
J. Biol. Chem. 266:15992-15998(1991) [PubMed] [Europe PMC] [Abstract]
"Molecular basis of apparent isolated 17,20-lyase deficiency: compound heterozygous mutations in the C-terminal region (Arg(496)-->Cys, Gln(461)-->Stop) actually cause combined 17 alpha-hydroxylase/17,20-lyase deficiency."
Biochim. Biophys. Acta 1139:275-279(1992) [PubMed] [Europe PMC] [Abstract]
"Compound heterozygous mutations (Arg 239-->Stop, Pro 342-->Thr) in the CYP17 (P45017 alpha) gene lead to ambiguous external genitalia in a male patient with partial combined 17 alpha-hydroxylase/17,20-lyase deficiency."
J. Clin. Endocrinol. Metab. 74:667-672(1992) [PubMed] [Europe PMC] [Abstract]
"Expression and purification of functional human 17 alpha-hydroxylase/17,20-lyase (P450c17) in Escherichia coli. Use of this system for study of a novel form of combined 17 alpha-hydroxylase/17,20-lyase deficiency."
J. Biol. Chem. 268:19681-19689(1993) [PubMed] [Europe PMC] [Abstract]
"Mutation of histidine 373 to leucine in cytochrome P450c17 causes 17 alpha-hydroxylase deficiency."
J. Biol. Chem. 268:25811-25817(1993) [PubMed] [Europe PMC] [Abstract]
"Deletion of amino acids Asp487-Ser488-Phe489 in human cytochrome P450c17 causes severe 17 alpha-hydroxylase deficiency."
J. Clin. Endocrinol. Metab. 77:489-493(1993) [PubMed] [Europe PMC] [Abstract]
"Point mutation of Arg440 to His in cytochrome P450c17 causes severe 17 alpha-hydroxylase deficiency."
J. Clin. Endocrinol. Metab. 79:160-164(1994) [PubMed] [Europe PMC] [Abstract]
"Mutation R96W in cytochrome P450c17 gene causes combined 17 alpha-hydroxylase/17-20-lyase deficiency in two French Canadian patients."
J. Clin. Endocrinol. Metab. 81:264-268(1996) [PubMed] [Europe PMC] [Abstract]
"The molecular basis of isolated 17,20 lyase deficiency."
Pediatr. Res. 39:89A-89A(1996)
"17alpha-hydroxylase/17,20-lyase deficiency as a model to study enzymatic activity regulation: role of phosphorylation."
J. Clin. Endocrinol. Metab. 85:1226-1231(2000) [PubMed] [Europe PMC] [Abstract]
"Pitfalls in characterizing P450c17 mutations associated with isolated 17,20-lyase deficiency."
J. Clin. Endocrinol. Metab. 86:4416-4423(2001) [PubMed] [Europe PMC] [Abstract]
"Combined 17alpha-hydroxylase/17,20-lyase deficiency caused by Phe93Cys mutation in the CYP17 gene."
J. Clin. Endocrinol. Metab. 87:898-905(2002) [PubMed] [Europe PMC] [Abstract]
"Differential inhibition of 17alpha-hydroxylase and 17,20-lyase activities by three novel missense CYP17 mutations identified in patients with P450c17 deficiency."
J. Clin. Endocrinol. Metab. 87:5714-5721(2002) [PubMed] [Europe PMC] [Abstract]
"P450c17 deficiency in Brazilian patients: biochemical diagnosis through progesterone levels confirmed by CYP17 genotyping."
J. Clin. Endocrinol. Metab. 88:5739-5746(2003) [PubMed] [Europe PMC] [Abstract]
"Novel CYP17A1 mutation in a Japanese patient with combined 17alpha-hydroxylase/17,20-lyase deficiency."
Metabolism 59:275-278(2010) [PubMed] [Europe PMC] [Abstract]
"Congenital adrenal hyperplasia due to 17-alpha-hydoxylase/17,20-lyase deficiency presenting with hypertension and pseudohermaphroditism: first case report from Oman."
Oman Med. J. 29:55-59(2014) [PubMed] [Europe PMC] [Abstract]
"A review of the literature on common CYP17A1 mutations in adults with 17-hydroxylase/17,20-lyase deficiency, a case series of such mutations among Koreans and functional characteristics of a novel mutation."
Metabolism 63:42-49(2014) [PubMed] [Europe PMC] [Abstract]
"Partial deficiency of 17alpha-hydroxylase/17,20-lyase caused by a novel missense mutation in the canonical cytochrome heme-interacting motif."
Eur. J. Endocrinol. 172:K19-25(2015) [PubMed] [Europe PMC] [Abstract]