E1A Binding Protein P300 (EP300)

KAT3B; p300; Histone Acetyltransferase p300

SPN	U628
HGNC	3373
MGI	1276116
PubMed	10700188
RGD	620036
UniProt	Q09472
Wiki	EP300

E1A binding protein p300 also known as EP300 or p300 is a protein which in humans is encoded by the EP300 gene.This protein regulates the activity of many genes in tissues throughout the body. It plays an essential role in regulating cell growth and division, prompting cells to mature and assume specialized functions (differentiate), and preventing the growth of cancerous tumors. The p300 protein appears to be critical for normal development before and after birth.
The p300 protein carries out its function by activating transcription, the process of making a blueprint of a gene for protein production. Specifically, p300 connects transcription factors, which are proteins that start the transcription process, with the complex of proteins that carries out transcription in the cell's nucleus.

Reference

"Molecular cloning and functional analysis of the adenovirus E1A-associated 300-kD protein (p300) reveals a protein with properties of a transcriptional adaptor."
Genes Dev. 8:869-884(1994) [PubMed] [Europe PMC] [Abstract]

"The DNA sequence of human chromosome 22."
Nature 402:489-495(1999) [PubMed] [Europe PMC] [Abstract]

"MOZ is fused to p300 in an acute monocytic leukemia with t(8;22)."
Genes Chromosomes Cancer 28:138-144(2000) [PubMed] [Europe PMC] [Abstract]

"Adenoviral E1A-associated protein p300 as a functional homologue of the transcriptional co-activator CBP."
Nature 374:85-88(1995) [PubMed] [Europe PMC] [Abstract]

"A transcriptional switch mediated by cofactor methylation."
Science 294:2507-2511(2001) [PubMed] [Europe PMC] [Abstract]

"The transcriptional coactivators p300 and CBP are histone acetyltransferases."
Cell 87:953-959(1996) [PubMed] [Europe PMC] [Abstract]

"A p300/CBP-associated factor that competes with the adenoviral oncoprotein E1A."
Nature 382:319-324(1996) [PubMed] [Europe PMC] [Abstract]

"An essential role for p300/CBP in the cellular response to hypoxia."
Proc. Natl. Acad. Sci. U.S.A. 93:12969-12973(1996) [PubMed] [Europe PMC] [Abstract]

"Differential transcriptional activation by human T-cell leukemia virus type 1 Tax mutants is mediated by distinct interactions with CREB binding protein and p300."
Mol. Cell. Biol. 18:2392-2405(1998) [PubMed] [Europe PMC] [Abstract]

"Chromatin remodelling by the glucocorticoid receptor requires the BRG1 complex."
Nature 393:88-91(1998) [PubMed] [Europe PMC] [Abstract]

"HIV-1 tat transcriptional activity is regulated by acetylation."
EMBO J. 18:6106-6118(1999) [PubMed] [Europe PMC] [Abstract]

"Functional role of p35srj, a novel p300/CBP binding protein, during transactivation by HIF-1."
Genes Dev. 13:64-75(1999) [PubMed] [Europe PMC] [Abstract]

"Exogenous expression of a dominant negative RORalpha1 vector in muscle cells impairs differentiation: RORalpha1 directly interacts with p300 and myoD."
Nucleic Acids Res. 27:411-420(1999) [PubMed] [Europe PMC] [Abstract]

"The MSG1 non-DNA-binding transactivator binds to the p300/CBP coactivators, enhancing their functional link to the Smad transcription factors."
J. Biol. Chem. 275:8825-8834(2000) [PubMed] [Europe PMC] [Abstract]

"A novel transcriptional repression domain mediates p21(WAF1/CIP1) induction of p300 transactivation."
Mol. Cell. Biol. 20:2676-2686(2000) [PubMed] [Europe PMC] [Abstract]

Erratum
Mol. Cell. Biol. 20:5360-5360(2000)

"Cells degrade a novel inhibitor of differentiation with E1A-like properties upon exiting the cell cycle."
Mol. Cell. Biol. 20:8889-8902(2000) [PubMed] [Europe PMC] [Abstract]

"A novel Rb- and p300-binding protein inhibits transactivation by MyoD."
Mol. Cell. Biol. 20:8903-8915(2000) [PubMed] [Europe PMC] [Abstract]

"Thyroid hormone receptor-binding protein, an LXXLL motif-containing protein, functions as a general coactivator."
Proc. Natl. Acad. Sci. U.S.A. 97:6212-6217(2000) [PubMed] [Europe PMC] [Abstract]

"Acetylation of HIV-1 Tat by CBP/P300 increases transcription of integrated HIV-1 genome and enhances binding to core histones."
Virology 277:278-295(2000) [PubMed] [Europe PMC] [Abstract]

"Selective coactivation of estrogen-dependent transcription by CITED1 CBP/p300-binding protein."
Genes Dev. 15:2598-2612(2001) [PubMed] [Europe PMC] [Abstract]

"Regulation of transcription by AMP-activated protein kinase: phosphorylation of p300 blocks its interaction with nuclear receptors."
J. Biol. Chem. 276:38341-38344(2001) [PubMed] [Europe PMC] [Abstract]

"Adenovirus DNA binding protein interacts with the SNF2-related CBP activator protein (SrCap) and inhibits SrCap-mediated transcription."
J. Virol. 75:10033-10040(2001) [PubMed] [Europe PMC] [Abstract]

"The oncoprotein Tax binds the SRC-1-interacting domain of CBP/p300 to mediate transcriptional activation."
Mol. Cell. Biol. 21:5520-5530(2001) [PubMed] [Europe PMC] [Abstract]

"Human T-lymphotropic virus type 1 p30(II) regulates gene transcription by binding CREB binding protein/p300."
J. Virol. 75:9885-9895(2001) [PubMed] [Europe PMC] [Abstract]

"A novel zinc finger protein TReP-132 interacts with CBP/p300 to regulate human CYP11A1 gene expression."
J. Biol. Chem. 276:33881-33892(2001) [PubMed] [Europe PMC] [Abstract]

"Molecular cloning and characterization of PELP1, a novel human coregulator of estrogen receptor alpha."
J. Biol. Chem. 276:38272-38279(2001) [PubMed] [Europe PMC] [Abstract]

"Role of Deltex-1 as a transcriptional regulator downstream of the Notch receptor."
J. Biol. Chem. 276:45031-45040(2001) [PubMed] [Europe PMC] [Abstract]

"Regulation of human flap endonuclease-1 activity by acetylation through the transcriptional coactivator p300."
Mol. Cell 7:1221-1231(2001) [PubMed] [Europe PMC] [Abstract]

"Interaction between the hematopoietic Ets transcription factor Spi-B and the coactivator CREB-binding protein associated with negative cross-talk with c-Myb."
Cell Growth Differ. 13:69-75(2002) [PubMed] [Europe PMC] [Abstract]

"Human CREB-binding protein/p300-interacting transactivator with ED-rich tail (CITED) 4, a new member of the CITED family, functions as a co-activator for transcription factor AP-2."
J. Biol. Chem. 277:8559-8565(2002) [PubMed] [Europe PMC] [Abstract]

"Synergy among nuclear receptor coactivators: selective requirement for protein methyltransferase and acetyltransferase activities."
Mol. Cell. Biol. 22:3621-3632(2002) [PubMed] [Europe PMC] [Abstract]

"Acetylation inactivates the transcriptional repressor BCL6."
Nat. Genet. 32:606-613(2002) [PubMed] [Europe PMC] [Abstract]

"p29ING4 and p28ING5 bind to p53 and p300, and enhance p53 activity."
Cancer Res. 63:2373-2378(2003) [PubMed] [Europe PMC] [Abstract]

"Identification of a promoter-specific transcriptional activation domain at the C-terminus of the Wnt effector protein T-cell factor 4."
J. Biol. Chem. 278:3776-3785(2003) [PubMed] [Europe PMC] [Abstract]

"Physical and functional interactions among AP-2 transcription factors, p300/CREB-binding protein, and CITED2."
J. Biol. Chem. 278:16021-16029(2003) [PubMed] [Europe PMC] [Abstract]

"Acetylated SP3 is a transcriptional activator."
J. Biol. Chem. 278:35775-35780(2003) [PubMed] [Europe PMC] [Abstract]

"P300/CBP acts as a coactivator to cartilage homeoprotein-1 (Cart1), paired-like homeoprotein, through acetylation of the conserved lysine residue adjacent to the homeodomain."
J. Bone Miner. Res. 18:1419-1429(2003) [PubMed] [Europe PMC] [Abstract]

"SATB1 makes a complex with p300 and represses gp91(phox) promoter activity."
Microbiol. Immunol. 47:803-811(2003) [PubMed] [Europe PMC] [Abstract]

"P300 transcriptional repression is mediated by SUMO modification."
Mol. Cell 11:1043-1054(2003) [PubMed] [Europe PMC] [Abstract]

"Synergism between p68 RNA helicase and the transcriptional coactivators CBP and p300."
Oncogene 22:151-156(2003) [PubMed] [Europe PMC] [Abstract]

"Ordered cooperative functions of PRMT1, p300, and CARM1 in transcriptional activation by p53."
Cell 117:735-748(2004) [PubMed] [Europe PMC] [Abstract]

"Regulation of human SRY subcellular distribution by its acetylation/deacetylation."
EMBO J. 23:3336-3345(2004) [PubMed] [Europe PMC] [Abstract]

"Histone acetyltransferase-dependent chromatin remodeling and the vascular clock."
J. Biol. Chem. 279:7091-7097(2004) [PubMed] [Europe PMC] [Abstract]

"Positive and negative modulation of the transcriptional activity of the ETS factor ESE-1 through interaction with p300, CREB-binding protein, and Ku 70/86."
J. Biol. Chem. 279:25241-25250(2004) [PubMed] [Europe PMC] [Abstract]

"Interferon regulatory factor 1 binding to p300 stimulates DNA-dependent acetylation of p53."
Mol. Cell. Biol. 24:10083-10098(2004) [PubMed] [Europe PMC] [Abstract]

"Regulation of the p300 HAT domain via a novel activation loop."
Nat. Struct. Mol. Biol. 11:308-315(2004) [PubMed] [Europe PMC] [Abstract]

"Orphan nuclear receptor small heterodimer partner, a novel corepressor for a basic helix-loop-helix transcription factor BETA2/neuroD."
Mol. Endocrinol. 18:776-790(2004) [PubMed] [Europe PMC] [Abstract]

"Dendrite development regulated by CREST, a calcium-regulated transcriptional activator."
Science 303:197-202(2004) [PubMed] [Europe PMC] [Abstract]

"Genetic heterogeneity in Rubinstein-Taybi syndrome: mutations in both the CBP and EP300 genes cause disease."
Am. J. Hum. Genet. 76:572-580(2005) [PubMed] [Europe PMC] [Abstract]

"SIRT1 deacetylation and repression of p300 involves lysine residues 1020/1024 within the cell cycle regulatory domain 1."
J. Biol. Chem. 280:10264-10276(2005) [PubMed] [Europe PMC] [Abstract]

"The coactivator p300 directly acetylates the forkhead transcription factor Foxo1 and stimulates Foxo1-induced transcription."
Mol. Endocrinol. 19:2283-2298(2005) [PubMed] [Europe PMC] [Abstract]

"Regulation of coactivator complex assembly and function by protein arginine methylation and demethylimination."
Proc. Natl. Acad. Sci. U.S.A. 102:3611-3616(2005) [PubMed] [Europe PMC] [Abstract]

"Nuclear Rho kinase, ROCK2, targets p300 acetyltransferase."
J. Biol. Chem. 281:15320-15329(2006) [PubMed] [Europe PMC] [Abstract]

"The transcriptional activity of CITED1 is regulated by phosphorylation in a cell cycle-dependent manner."
J. Biol. Chem. 281:27426-27435(2006) [PubMed] [Europe PMC] [Abstract]

"Kinetic and mass spectrometric analysis of p300 histone acetyltransferase domain autoacetylation."
J. Biol. Chem. 281:40292-40301(2006) [PubMed] [Europe PMC] [Abstract]

"HDAC1 acetylation is linked to progressive modulation of steroid receptor-induced gene transcription."
Mol. Cell 22:669-679(2006) [PubMed] [Europe PMC] [Abstract]

"Sp1 deacetylation induced by phorbol ester recruits p300 to activate 12(S)-lipoxygenase gene transcription."
Mol. Cell. Biol. 26:1770-1785(2006) [PubMed] [Europe PMC] [Abstract]

"MTA1, a transcriptional activator of breast cancer amplified sequence 3."
Proc. Natl. Acad. Sci. U.S.A. 103:6670-6675(2006) [PubMed] [Europe PMC] [Abstract]

Erratum
Proc. Natl. Acad. Sci. U.S.A. 110:4147-4148(2013)

"Critical and functional regulation of CHOP (C/EBP homologous protein) through the N-terminal portion."
J. Biol. Chem. 282:35687-35694(2007) [PubMed] [Europe PMC] [Abstract]

"Concerted activation of the Mdm2 promoter by p72 RNA helicase and the coactivators p300 and P/CAF."
J. Cell. Biochem. 101:1252-1265(2007) [PubMed] [Europe PMC] [Abstract]

"Acetylation of Sirt2 by p300 attenuates its deacetylase activity."
Biochem. Biophys. Res. Commun. 375:576-580(2008) [PubMed] [Europe PMC] [Abstract]

"PEBP2-beta/CBF-beta-dependent phosphorylation of RUNX1 and p300 by HIPK2: implications for leukemogenesis."
Blood 112:3777-3787(2008) [PubMed] [Europe PMC] [Abstract]

"PML activates transcription by protecting HIPK2 and p300 from SCFFbx3-mediated degradation."
Mol. Cell. Biol. 28:7126-7138(2008) [PubMed] [Europe PMC] [Abstract]

"The SIRT2 deacetylase regulates autoacetylation of p300."
Mol. Cell 32:449-455(2008) [PubMed] [Europe PMC] [Abstract]

"A quantitative atlas of mitotic phosphorylation."
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]

"SENP3 is responsible for HIF-1 transactivation under mild oxidative stress via p300 de-SUMOylation."
EMBO J. 28:2748-2762(2009) [PubMed] [Europe PMC] [Abstract]

"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]

"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]

"Cyclin-dependent kinase-9 is a component of the p300/GATA4 complex required for phenylephrine-induced hypertrophy in cardiomyocytes."
J. Biol. Chem. 285:9556-9568(2010) [PubMed] [Europe PMC] [Abstract]

"Regulation of unfolded protein response modulator XBP1s by acetylation and deacetylation."
Biochem. J. 433:245-252(2011) [PubMed] [Europe PMC] [Abstract]

"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]

"Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]

"Human ALKBH4 interacts with proteins associated with transcription."
PLoS ONE 7:E49045-E49045(2012) [PubMed] [Europe PMC] [Abstract]

"Regulation of transcription through acetylation of H3K122 on the lateral surface of the histone octamer."
Cell 152:859-872(2013) [PubMed] [Europe PMC] [Abstract]

"A hybrid mechanism of action for BCL6 in B cells defined by formation of functionally distinct complexes at enhancers and promoters."
Cell Rep. 4:578-588(2013) [PubMed] [Europe PMC] [Abstract]

"Kruppel-like factor 15 is critical for vascular inflammation."
J. Clin. Invest. 123:4232-4241(2013) [PubMed] [Europe PMC] [Abstract]

"Differential regulation of estrogen receptor alpha expression in breast cancer cells by metastasis-associated protein 1."
Cancer Res. 74:1484-1494(2014) [PubMed] [Europe PMC] [Abstract]

"Constitutive nuclear localization of an alternatively spliced sirtuin-2 isoform."
J. Mol. Biol. 426:1677-1691(2014) [PubMed] [Europe PMC] [Abstract]

"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]

"Modification of ASC1 by UFM1 is crucial for ERalpha transactivation and breast cancer development."
Mol. Cell 56:261-274(2014) [PubMed] [Europe PMC] [Abstract]

"CBP and p300 acetylate PCNA to link its degradation with nucleotide excision repair synthesis."
Nucleic Acids Res. 42:8433-8448(2014) [PubMed] [Europe PMC] [Abstract]

"Two ZNF509 (ZBTB49) isoforms induce cell-cycle arrest by activating transcription of p21/CDKN1A and RB upon exposure to genotoxic stress."
Nucleic Acids Res. 42:11447-11461(2014) [PubMed] [Europe PMC] [Abstract]

"Structural basis for recruitment of CBP/p300 by hypoxia-inducible factor-1 alpha."
Proc. Natl. Acad. Sci. U.S.A. 99:5367-5372(2002) [PubMed] [Europe PMC] [Abstract]

"Structural basis for negative regulation of hypoxia-inducible factor-1alpha by CITED2."
Nat. Struct. Biol. 10:504-512(2003) [PubMed] [Europe PMC] [Abstract]

"The structural basis of protein acetylation by the p300/CBP transcriptional coactivator."
Nature 451:846-850(2008) [PubMed] [Europe PMC] [Abstract]

"Structural basis for p300 Taz2-p53 TAD1 binding and modulation by phosphorylation."
Structure 17:202-210(2009) [PubMed] [Europe PMC] [Abstract]

"Histone recognition and large-scale structural analysis of the human bromodomain family."
Cell 149:214-231(2012) [PubMed] [Europe PMC] [Abstract]

"Structure of the p300 catalytic core and implications for chromatin targeting and HAT regulation."
Nat. Struct. Mol. Biol. 20:1040-1046(2013) [PubMed] [Europe PMC] [Abstract]

"Structure of the p300 histone acetyltransferase bound to acetyl-coenzyme A and its analogues."
Biochemistry 53:3415-3422(2014) [PubMed] [Europe PMC] [Abstract]

"Mutations truncating the EP300 acetylase in human cancers."
Nat. Genet. 24:300-303(2000) [PubMed] [Europe PMC] [Abstract]