Actinin Alpha 4 (ACTN4)

FSGS; FSGS1; F-actin cross-linking protein; Non-muscle alpha-actinin 4

SPN	U621
HGNC	166
MGI	1890773
PubMed	22348389
RGD	61816
UniProt	O43707
Wiki	ACTN4

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments.

Alpha-actinin-4 is a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis.

Organism species: Homo sapiens (Human)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPU621Hu01	Recombinant Actinin Alpha 4 (ACTN4)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAU621Hu01	Monoclonal Antibody to Actinin Alpha 4 (ACTN4)	WB; IHC; ICC; IP.
Antibodies	PAU621Hu01	Polyclonal Antibody to Actinin Alpha 4 (ACTN4)	WB; IHC; ICC; IF.
Assay Kits	CLU621Hu01	CLIA Kit for Actinin Alpha 4 (ACTN4)	CLIA assay for Antigen Detection
Assay Kits	ELU621Hu01	ELISA Kit for Actinin Alpha 4 (ACTN4)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Mus musculus (Mouse)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPU621Mu01	Recombinant Actinin Alpha 4 (ACTN4)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAU621Mu01	Monoclonal Antibody to Actinin Alpha 4 (ACTN4)	WB; IHC; ICC; IP.
Antibodies	PAU621Mu01	Polyclonal Antibody to Actinin Alpha 4 (ACTN4)	WB; IHC; ICC; IF.
Assay Kits	CLU621Mu01	CLIA Kit for Actinin Alpha 4 (ACTN4)	CLIA assay for Antigen Detection
Assay Kits	ELU621Mu01	ELISA Kit for Actinin Alpha 4 (ACTN4)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Rattus norvegicus (Rat)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPU621Ra01	Recombinant Actinin Alpha 4 (ACTN4)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAU621Ra01	Monoclonal Antibody to Actinin Alpha 4 (ACTN4)	WB; IHC; ICC; IP.
Antibodies	PAU621Ra01	Polyclonal Antibody to Actinin Alpha 4 (ACTN4)	WB; IHC; ICC; IF.
Assay Kits	CLU621Ra01	CLIA Kit for Actinin Alpha 4 (ACTN4)	CLIA assay for Antigen Detection
Assay Kits	ELU621Ra01	ELISA Kit for Actinin Alpha 4 (ACTN4)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Canis familiaris; Canine (Dog)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPU621Ca01	Recombinant Actinin Alpha 4 (ACTN4)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAU621Ca01	Monoclonal Antibody to Actinin Alpha 4 (ACTN4)	WB; IHC; ICC; IP.
Antibodies	PAU621Ca01	Polyclonal Antibody to Actinin Alpha 4 (ACTN4)	WB; IHC; ICC; IF.
Assay Kits	CLU621Ca01	CLIA Kit for Actinin Alpha 4 (ACTN4)	CLIA assay for Antigen Detection
Assay Kits	ELU621Ca01	ELISA Kit for Actinin Alpha 4 (ACTN4)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Sus scrofa; Porcine (Pig)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPU621Po01	Recombinant Actinin Alpha 4 (ACTN4)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAU621Po01	Monoclonal Antibody to Actinin Alpha 4 (ACTN4)	WB; IHC; ICC; IP.
Antibodies	PAU621Po01	Polyclonal Antibody to Actinin Alpha 4 (ACTN4)	WB; IHC; ICC; IF.
Assay Kits	CLU621Po01	CLIA Kit for Actinin Alpha 4 (ACTN4)	CLIA assay for Antigen Detection
Assay Kits	ELU621Po01	ELISA Kit for Actinin Alpha 4 (ACTN4)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Bos taurus; Bovine (Cattle)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPU621Bo01	Recombinant Actinin Alpha 4 (ACTN4)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAU621Bo01	Monoclonal Antibody to Actinin Alpha 4 (ACTN4)	WB; IHC; ICC; IP.
Antibodies	PAU621Bo01	Polyclonal Antibody to Actinin Alpha 4 (ACTN4)	WB; IHC; ICC; IF.
Assay Kits	CLU621Bo01	CLIA Kit for Actinin Alpha 4 (ACTN4)	CLIA assay for Antigen Detection
Assay Kits	ELU621Bo01	ELISA Kit for Actinin Alpha 4 (ACTN4)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Oryctolagus cuniculus (Rabbit)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPU621Rb01	Recombinant Actinin Alpha 4 (ACTN4)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAU621Rb01	Monoclonal Antibody to Actinin Alpha 4 (ACTN4)	WB; IHC; ICC; IP.
Antibodies	PAU621Rb01	Polyclonal Antibody to Actinin Alpha 4 (ACTN4)	WB; IHC; ICC; IF.
Assay Kits	CLU621Rb01	CLIA Kit for Actinin Alpha 4 (ACTN4)	CLIA assay for Antigen Detection
Assay Kits	ELU621Rb01	ELISA Kit for Actinin Alpha 4 (ACTN4)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Rhesus monkey (Simian)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPU621Mo01	Recombinant Actinin Alpha 4 (ACTN4)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAU621Mo01	Monoclonal Antibody to Actinin Alpha 4 (ACTN4)	WB; IHC; ICC; IP.
Antibodies	PAU621Mo01	Polyclonal Antibody to Actinin Alpha 4 (ACTN4)	WB; IHC; ICC; IF.
Assay Kits	CLU621Mo01	CLIA Kit for Actinin Alpha 4 (ACTN4)	CLIA assay for Antigen Detection
Assay Kits	ELU621Mo01	ELISA Kit for Actinin Alpha 4 (ACTN4)	Enzyme-linked immunosorbent assay for Antigen Detection

Reference

"The human non-muscle alpha-actinin protein encoded by the ACTN4 gene suppresses tumorigenicity of human neuroblastoma cells."
Oncogene 19:380-386(2000) [PubMed] [Europe PMC] [Abstract]

"Novel splicing isoform of actin-binding protein alpha-actinin 4 in epidermoid carcinoma cells A431."
Tsitologiia 54:25-32(2012) [PubMed] [Europe PMC] [Abstract]

"The DNA sequence and biology of human chromosome 19."
Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"The full-ORF clone resource of the German cDNA consortium."
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]

"Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes."
Genome Res. 16:55-65(2006) [PubMed] [Europe PMC] [Abstract]

"Actinin-4, a novel actin-bundling protein associated with cell motility and cancer invasion."
J. Cell Biol. 140:1383-1393(1998) [PubMed] [Europe PMC] [Abstract]

Erratum
J. Cell Biol. 143:276-276(1998)

"Interaction of two actin-binding proteins, synaptopodin and alpha-actinin-4, with the tight junction protein MAGI-1."
J. Biol. Chem. 277:30183-30190(2002) [PubMed] [Europe PMC] [Abstract]

"CART: an Hrs/actinin-4/BERP/myosin V protein complex required for efficient receptor recycling."
Mol. Biol. Cell 16:2470-2482(2005) [PubMed] [Europe PMC] [Abstract]

"Molecular composition of IMP1 ribonucleoprotein granules."
Mol. Cell. Proteomics 6:798-811(2007) [PubMed] [Europe PMC] [Abstract]

"Tryptic digestion of ubiquitin standards reveals an improved strategy for identifying ubiquitinated proteins by mass spectrometry."
Proteomics 7:868-874(2007) [PubMed] [Europe PMC] [Abstract]

"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]

"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]

"Familial focal segmental glomerulosclerosis (FSGS)-linked alpha-actinin 4 (ACTN4) protein mutants lose ability to activate transcription by nuclear hormone receptors."
J. Biol. Chem. 287:12027-12035(2012) [PubMed] [Europe PMC] [Abstract]

"Mutations in ACTN4, encoding alpha-actinin-4, cause familial focal segmental glomerulosclerosis."
Nat. Genet. 24:251-256(2000) [PubMed] [Europe PMC] [Abstract]

"Familial focal segmental glomerulosclerosis associated with an ACTN4 mutation and paternal germline mosaicism."
Am. J. Kidney Dis. 51:834-838(2008) [PubMed] [Europe PMC] [Abstract]

"Mutational analysis of ACTN4, encoding alpha-actinin 4, in patients with focal segmental glomerulosclerosis using HRM method."
Folia Biol. (Praha) 59:110-115(2013) [PubMed] [Europe PMC] [Abstract]