Choline Kinase Alpha (CHKa)
CHK-A; CHK; CKI; EK; Choline Kinase; Ethanolamine kinase
The major pathway for the biosynthesis of phosphatidylcholine occurs via the CDP-choline pathway. Choline kinase alpha is the initial enzyme in the sequence and may play a regulatory role. The encoded protein also catalyzes the phosphorylation of ethanolamine. Two transcript variants encoding different isoforms have been found for this gene.In melanocytic cells CHKA gene expression may be regulated by MITF.
The deduced sequence of the human enzyme comprised 456 amino acids with a calculated relative molecular mass of 52,065. The human enzyme resembled the rat liver enzyme over the entire sequence. It also resembled the yeast enzyme in the carboxy-terminal region, but not in the amino-terminal region.
Organism species: Homo sapiens (Human)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPU472Hu01 | Recombinant Choline Kinase Alpha (CHKa) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAU472Hu01 | Monoclonal Antibody to Choline Kinase Alpha (CHKa) | WB; IHC; ICC; IP. |
| PAU472Hu01 | Polyclonal Antibody to Choline Kinase Alpha (CHKa) | WB; IHC; ICC; IF. | |
| Assay Kits | CLU472Hu01 | CLIA Kit for Choline Kinase Alpha (CHKa) | CLIA assay for Antigen Detection |
| ELU472Hu01 | ELISA Kit for Choline Kinase Alpha (CHKa) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Mus musculus (Mouse)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPU472Mu01 | Recombinant Choline Kinase Alpha (CHKa) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAU472Mu01 | Monoclonal Antibody to Choline Kinase Alpha (CHKa) | WB; IHC; ICC; IP. |
| PAU472Mu01 | Polyclonal Antibody to Choline Kinase Alpha (CHKa) | WB; IHC; ICC; IF. | |
| Assay Kits | CLU472Mu01 | CLIA Kit for Choline Kinase Alpha (CHKa) | CLIA assay for Antigen Detection |
| ELU472Mu01 | ELISA Kit for Choline Kinase Alpha (CHKa) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Rattus norvegicus (Rat)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPU472Ra01 | Recombinant Choline Kinase Alpha (CHKa) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAU472Ra01 | Monoclonal Antibody to Choline Kinase Alpha (CHKa) | WB; IHC; ICC; IP. |
| PAU472Ra01 | Polyclonal Antibody to Choline Kinase Alpha (CHKa) | WB; IHC; ICC; IF. | |
| Assay Kits | CLU472Ra01 | CLIA Kit for Choline Kinase Alpha (CHKa) | CLIA assay for Antigen Detection |
| ELU472Ra01 | ELISA Kit for Choline Kinase Alpha (CHKa) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Canis familiaris; Canine (Dog)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPU472Ca01 | Recombinant Choline Kinase Alpha (CHKa) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAU472Ca01 | Monoclonal Antibody to Choline Kinase Alpha (CHKa) | WB; IHC; ICC; IP. |
| PAU472Ca01 | Polyclonal Antibody to Choline Kinase Alpha (CHKa) | WB; IHC; ICC; IF. | |
| Assay Kits | CLU472Ca01 | CLIA Kit for Choline Kinase Alpha (CHKa) | CLIA assay for Antigen Detection |
| ELU472Ca01 | ELISA Kit for Choline Kinase Alpha (CHKa) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Sus scrofa; Porcine (Pig)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPU472Po01 | Recombinant Choline Kinase Alpha (CHKa) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAU472Po01 | Monoclonal Antibody to Choline Kinase Alpha (CHKa) | WB; IHC; ICC; IP. |
| PAU472Po01 | Polyclonal Antibody to Choline Kinase Alpha (CHKa) | WB; IHC; ICC; IF. | |
| Assay Kits | CLU472Po01 | CLIA Kit for Choline Kinase Alpha (CHKa) | CLIA assay for Antigen Detection |
| ELU472Po01 | ELISA Kit for Choline Kinase Alpha (CHKa) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Bos taurus; Bovine (Cattle)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPU472Bo01 | Recombinant Choline Kinase Alpha (CHKa) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAU472Bo01 | Monoclonal Antibody to Choline Kinase Alpha (CHKa) | WB; IHC; ICC; IP. |
| PAU472Bo01 | Polyclonal Antibody to Choline Kinase Alpha (CHKa) | WB; IHC; ICC; IF. | |
| Assay Kits | CLU472Bo01 | CLIA Kit for Choline Kinase Alpha (CHKa) | CLIA assay for Antigen Detection |
| ELU472Bo01 | ELISA Kit for Choline Kinase Alpha (CHKa) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Oryctolagus cuniculus (Rabbit)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPU472Rb01 | Recombinant Choline Kinase Alpha (CHKa) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAU472Rb01 | Monoclonal Antibody to Choline Kinase Alpha (CHKa) | WB; IHC; ICC; IP. |
| PAU472Rb01 | Polyclonal Antibody to Choline Kinase Alpha (CHKa) | WB; IHC; ICC; IF. | |
| Assay Kits | CLU472Rb01 | CLIA Kit for Choline Kinase Alpha (CHKa) | CLIA assay for Antigen Detection |
| ELU472Rb01 | ELISA Kit for Choline Kinase Alpha (CHKa) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Rhesus monkey (Simian)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPU472Mo01 | Recombinant Choline Kinase Alpha (CHKa) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAU472Mo01 | Monoclonal Antibody to Choline Kinase Alpha (CHKa) | WB; IHC; ICC; IP. |
| PAU472Mo01 | Polyclonal Antibody to Choline Kinase Alpha (CHKa) | WB; IHC; ICC; IF. | |
| Assay Kits | CLU472Mo01 | CLIA Kit for Choline Kinase Alpha (CHKa) | CLIA assay for Antigen Detection |
| ELU472Mo01 | ELISA Kit for Choline Kinase Alpha (CHKa) | Enzyme-linked immunosorbent assay for Antigen Detection |
Reference
"Cloning of a human choline kinase cDNA by complementation of the yeast cki mutation."
FEBS Lett. 304:229-232(1992) [PubMed] [Europe PMC] [Abstract]
"Human chromosome 11 DNA sequence and analysis including novel gene identification."
Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract]
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
"Differential role of human choline kinase alpha and beta enzymes in lipid metabolism: implications in cancer onset and treatment."
PLoS ONE 4:E7819-E7819(2009) [PubMed] [Europe PMC] [Abstract]
"Elucidation of human choline kinase crystal structures in complex with the products ADP or phosphocholine."
J. Mol. Biol. 364:136-151(2006) [PubMed] [Europe PMC] [Abstract]
"Crystal structures of human choline kinase isoforms in complex with hemicholinium-3: single amino acid near the active site influences inhibitor sensitivity."
J. Biol. Chem. 285:16330-16340(2010) [PubMed] [Europe PMC] [Abstract]