Charcot Leyden Crystal Protein (CLC)

LGALS10; LPPL; Eosinophil Lysophospholipase; Lysolecithin Acylhydrolase; Galectin 10

SPN	U461
HGNC	2014
MGI	891997
PubMed	2455566
UniProt	Q05315
Wiki	CLC

CLC protein is a unique constituent of eosinophils and basophils. This protein forms the hexagonal bipyramidal crystals observed in tissues at sites of eosinophil accumulations, possesses lysophospholipase activity, and comprises an estimated 7% to 10% of total eosinophil protein. The ultrastructural localization of CLC protein was studied in mature peripheral blood eosinophils from normal donors and from patients with the idiopathic hypereosinophilic syndrome. Subcellular localization was evaluated by immunoelectron microscopy using eosinophils, both from buffy coat and purified cell suspensions, that were fixed by a variety of methods. Immunochemical detection of CLC protein employed rabbit antiserum to eosinophil CLC protein, affinity chromatography-purified monospecific IgG antibodies, and postembedding immunogold techniques.

Reference

"Molecular cloning and characterization of human eosinophil Charcot-Leyden crystal protein (lysophospholipase). Similarities to IgE binding proteins and the S-type animal lectin superfamily."
J. Immunol. 150:456-468(1993) [PubMed] [Europe PMC] [Abstract]

"Localization of the human eosinophil Charcot-Leyden crystal protein (lysophospholipase) gene (CLC) to chromosome 19 and the human ribonuclease 2 (eosinophil-derived neurotoxin) and ribonuclease 3 (eosinophil cationic protein) genes (RNS2 and RNS3) to chromosome 14."
Genomics 13:240-242(1992) [PubMed] [Europe PMC] [Abstract]

"The genomic structure of the human Charcot-Leyden crystal protein gene is analogous to those of the galectin genes."
Genomics 40:217-221(1997) [PubMed] [Europe PMC] [Abstract]

"A primate subfamily of galectins expressed at the maternal-fetal interface that promote immune cell death."
Proc. Natl. Acad. Sci. U.S.A. 106:9731-9736(2009) [PubMed] [Europe PMC] [Abstract]

"The DNA sequence and biology of human chromosome 19."
Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Comparative proteomics of nasal fluid in seasonal allergic rhinitis."
J. Proteome Res. 5:330-338(2006) [PubMed] [Europe PMC] [Abstract]

"Human CD4+CD25+ regulatory T cells: proteome analysis identifies galectin-10 as a novel marker essential for their anergy and suppressive function."
Blood 110:1550-1558(2007) [PubMed] [Europe PMC] [Abstract]

"Galectin-10, eosinophils, and celiac disease."
Ann. N. Y. Acad. Sci. 1173:357-364(2009) [PubMed] [Europe PMC] [Abstract]

"Galectin-10, a potential biomarker of eosinophilic airway inflammation."
PLoS ONE 7:E42549-E42549(2012) [PubMed] [Europe PMC] [Abstract]

"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]

"Crystal structure of human Charcot-Leyden crystal protein, an eosinophil lysophospholipase, identifies it as a new member of the carbohydrate-binding family of galectins."
Structure 3:1379-1393(1995) [PubMed] [Europe PMC] [Abstract]

"Selective recognition of mannose by the human eosinophil Charcot-Leyden crystal protein (galectin-10): a crystallographic study at 1.8 A resolution."
Biochemistry 38:13837-13843(1999) [PubMed] [Europe PMC] [Abstract]

"Charcot-Leyden crystal protein (galectin-10) is not a dual function galectin with lysophospholipase activity but binds a lysophospholipase inhibitor in a novel structural fashion."
J. Biol. Chem. 277:14859-14868(2002) [PubMed] [Europe PMC] [Abstract]