Cytosolic Ovarian Carcinoma Antigen 1 (COVA1)

APK1; tNOX; ENOX2; Ecto-NOX Disulfide-Thiol Exchanger 2; Tumor-associated hydroquinone oxidase; Hydroquinone [NADH] oxidase; Protein disulfide-thiol oxidoreductase

SPN	U390
HGNC	2259
MGI	2384799
PubMed	19734122
RGD	1563643
UniProt	Q16206
Wiki	ENOX2

is a growth-related cell surface protein. It was identifed because it reacts with the monoclonal antibody KI in cells, such as the ovarian carcinoma line OVCAR-3, also expressing the CAKI surface glycoprotein. The encoded protein has two enzymatic activities: catalysis of hydroquinone or NADH oxidation, and protein disulfide interchange. The two activities alternate with a period length of about 24 minutes. The encoded protein also displays prion-like properties. Two transcript variants encoding different isoforms have been found for this gene.The COVA1 protein contains 3 potential N-linked glycosylation sites and multiple potential phosphorylation sites. Northern blot analysis revealed expression of a major 3.8-kb transcript predominantly in heart, placenta, and skeletal muscle but also in lung, liver, kidney, and pancreas.

Reference

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Biochemistry 41:3732-3741(2002) [PubMed] [Europe PMC] [Abstract]

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"Molecular cloning and expression of a cDNA encoding a protein detected by the K1 antibody from an ovarian carcinoma (OVCAR-3) cell line."
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"Inhibition of plasma membrane NADH oxidase activity and growth of HeLa cells by natural and synthetic retinoids."
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"The sulfonylurea-inhibited NADH oxidase activity of HeLa cell plasma membranes has properties of a protein disulfide-thiol oxidoreductase with protein disulfide-thiol interchange activity."
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"The plasma membrane NADH oxidase of HeLa cells has hydroquinone oxidase activity."
Biochim. Biophys. Acta 1412:66-77(1999) [PubMed] [Europe PMC] [Abstract]

"Cancer isoform of a tumor-associated cell surface NADH oxidase (tNOX) has properties of a prion."
Biochemistry 40:7351-7354(2001) [PubMed] [Europe PMC] [Abstract]

"Surface NADH oxidase of HeLa cells lacks intrinsic membrane binding motifs."
Arch. Biochem. Biophys. 392:251-256(2001) [PubMed] [Europe PMC] [Abstract]

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