Legumain (LGMN)

AEP; LGMN1; PRSC1; Protease,Cysteine 1; Asparaginyl endopeptidase

SPN	U285
HGNC	9472
MGI	1330838
PubMed	22441772
RGD	619832
UniProt	Q99538
Wiki	LGMN

Legumain is a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined.

Reference

"Cloning, isolation, and characterization of mammalian legumain, an asparaginyl endopeptidase."
J. Biol. Chem. 272:8090-8098(1997) [PubMed] [Europe PMC] [Abstract]

"Molecular cloning of a human cDNA encoding putative cysteine protease (PRSC1) and its chromosome assignment to 14q32.1."
Cytogenet. Cell Genet. 74:120-123(1996) [PubMed] [Europe PMC] [Abstract]

"The DNA sequence and analysis of human chromosome 14."
Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Activation of human prolegumain by cleavage at a C-terminal asparagine residue."
Biochem. J. 352:327-334(2000) [PubMed] [Europe PMC] [Abstract]

"Autocatalytic activation of human legumain at aspartic acid residues."
FEBS Lett. 438:114-118(1998) [PubMed] [Europe PMC] [Abstract]

"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]

"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]

"Mechanistic and structural studies on legumain explain its zymogenicity, distinct activation pathways, and regulation."
Proc. Natl. Acad. Sci. U.S.A. 110:10940-10945(2013) [PubMed] [Europe PMC] [Abstract]