Leupaxin (LPXN)

LDPL; LDLP

SPN	U278
HGNC	14061
MGI	2147677
PubMed	12674328
RGD	1304981
UniProt	O60711
Wiki	LPXN

Leupaxin is preferentially expressed in hematopoietic cells and is most homologous to the focal adhesion protein, paxillin. It may function in cell type-specific signaling by associating with PYK2. As a substrate for a tyrosine kinase in lymphoid cells, this protein may also function in, and be regulated by tyrosine kinase activity. LPXN shares 37% overall amino acid sequence identity with the focal adhesion protein paxillin (PXN). The C terminus of LPXN contains 4 LIM domains that share 67 to 76% identity with the LIM domains of PXN. Its N terminus contains 3 LD motifs, which were first identified in PXN as 13-amino acid motifs with an invariable leucine and aspartate pair. LD motifs in PXN have been implicated in binding to cytoplasmic PTK2 and vinculin, resulting in localization of PTK2 to focal adhesions.

Reference

"Leupaxin is a novel LIM domain protein that forms a complex with PYK2."
J. Biol. Chem. 273:11709-11713(1998) [PubMed] [Europe PMC] [Abstract]

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

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Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Binding of paxillin to alpha4 integrins modifies integrin-dependent biological responses."
Nature 402:676-681(1999) [PubMed] [Europe PMC] [Abstract]

"Leupaxin is a critical adaptor protein in the adhesion zone of the osteoclast."
J. Bone Miner. Res. 18:669-685(2003) [PubMed] [Europe PMC] [Abstract]

"Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry."
Anal. Chem. 76:2763-2772(2004) [PubMed] [Europe PMC] [Abstract]

"Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]

"Interaction of Pyk2 and PTP-PEST with leupaxin in prostate cancer cells."
Am. J. Physiol. 292:C2288-C2296(2007) [PubMed] [Europe PMC] [Abstract]

"Leupaxin negatively regulates B cell receptor signaling."
J. Biol. Chem. 282:27181-27191(2007) [PubMed] [Europe PMC] [Abstract]

"The LIM protein leupaxin is enriched in smooth muscle and functions as an serum response factor cofactor to induce smooth muscle cell gene transcription."
Circ. Res. 102:1502-1511(2008) [PubMed] [Europe PMC] [Abstract]

"Leupaxin, a novel coactivator of the androgen receptor, is expressed in prostate cancer and plays a role in adhesion and invasion of prostate carcinoma cells."
Mol. Endocrinol. 22:1606-1621(2008) [PubMed] [Europe PMC] [Abstract]

"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]

"Leupaxin is similar to paxillin in focal adhesion targeting and tyrosine phosphorylation but has distinct roles in cell adhesion and spreading."
Cell Adh. Migr. 4:527-540(2010) [PubMed] [Europe PMC] [Abstract]

"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]

"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]

"Solution structure of the LIM domain of human leupaxin."
Submitted (JAN-2006) to the PDB data bank