Villin 1 (VIL1)

SPN	U256
HGNC	12690
MGI	98930
PubMed	22530999
RGD	1307048
UniProt	P09327
Wiki	Villin

Villin is a 92.5 kDa tissue-specific actin-binding protein associated with the actin core bundle of the brush border. Villin contains multiple gelsolin-like domains capped by a small (8.5 kDa) "headpiece" at the C-terminus consisting of a fast and independently-folding three-helix bundle that is stabilized by hydrophobic interactions. The headpiece domain is a commonly studied protein in molecular dynamics due to its small size and fast folding kinetics and short primary sequence.Villin is an actin binding protein expressed mainly in the brush border of the epithelium in vertebrates but sometimes it is ubiquitously expressed in protists and plants. Villin is found localized in the microvilli of the brush border of the epithelium lining of the gut and renal tubes in vertebrates.

Reference

"Sequence of human villin: a large duplicated domain homologous with other actin-severing proteins and a unique small carboxy-terminal domain related to villin specificity."
J. Cell Biol. 107:1759-1766(1988) [PubMed] [Europe PMC] [Abstract]

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"A human villin cDNA clone to investigate the differentiation of intestinal and kidney cells in vivo and in culture."
EMBO J. 5:3119-3124(1986) [PubMed] [Europe PMC] [Abstract]

"Different calcium dependence of the capping and cutting activities of villin."
J. Biol. Chem. 261:9274-9281(1986) [PubMed] [Europe PMC] [Abstract]

"Tyrosine phosphorylation of villin regulates the organization of the actin cytoskeleton."
J. Biol. Chem. 276:36163-36167(2001) [PubMed] [Europe PMC] [Abstract]

"Regulation of actin dynamics by tyrosine phosphorylation: identification of tyrosine phosphorylation sites within the actin-severing domain of villin."
Biochemistry 41:11750-11760(2002) [PubMed] [Europe PMC] [Abstract]

"Abnormalities in villin gene expression and canalicular microvillus structure in progressive cholestatic liver disease of childhood."
Lancet 362:1112-1119(2003) [PubMed] [Europe PMC] [Abstract]

"Association of villin with phosphatidylinositol 4,5-bisphosphate regulates the actin cytoskeleton."
J. Biol. Chem. 279:3096-3110(2004) [PubMed] [Europe PMC] [Abstract]

"Identification of a functional switch for actin severing by cytoskeletal proteins."
J. Biol. Chem. 279:24915-24918(2004) [PubMed] [Europe PMC] [Abstract]

"Functional dissection and molecular characterization of calcium-sensitive actin-capping and actin-depolymerizing sites in villin."
J. Biol. Chem. 279:45036-45046(2004) [PubMed] [Europe PMC] [Abstract]

"Regulation of cell motility by tyrosine phosphorylated villin."
Mol. Biol. Cell 15:4807-4817(2004) [PubMed] [Europe PMC] [Abstract]

"Interaction of phospholipase C-gamma1 with villin regulates epithelial cell migration."
J. Biol. Chem. 281:31972-31986(2006) [PubMed] [Europe PMC] [Abstract]

"Obligatory role for phospholipase C-gamma(1) in villin-induced epithelial cell migration."
Am. J. Physiol. 292:C1775-C1786(2007) [PubMed] [Europe PMC] [Abstract]

"Dimerization and actin-bundling properties of villin and its role in the assembly of epithelial cell brush borders."
J. Biol. Chem. 282:26528-26541(2007) [PubMed] [Europe PMC] [Abstract]

"Villin severing activity enhances actin-based motility in vivo."
Mol. Biol. Cell 18:827-838(2007) [PubMed] [Europe PMC] [Abstract]

"Autotaxin and lysophosphatidic acid stimulate intestinal cell motility by redistribution of the actin modifying protein villin to the developing lamellipodia."
Exp. Cell Res. 314:530-542(2008) [PubMed] [Europe PMC] [Abstract]

"A novel role for villin in intestinal epithelial cell survival and homeostasis."
J. Biol. Chem. 283:9454-9464(2008) [PubMed] [Europe PMC] [Abstract]

"Differential effects of lysophosphatidic acid and phosphatidylinositol 4,5-bisphosphate on actin dynamics by direct association with the actin-binding protein villin."
J. Biol. Chem. 284:35278-35282(2009) [PubMed] [Europe PMC] [Abstract]

"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]

"Solution structures of the C-terminal headpiece subdomains of human villin and advillin, evaluation of headpiece F-actin-binding requirements."
Protein Sci. 13:1276-1287(2004) [PubMed] [Europe PMC] [Abstract]

"Helix straightening as an activation mechanism in the gelsolin superfamily of actin regulatory proteins."
J. Biol. Chem. 284:21265-21269(2009) [PubMed] [Europe PMC] [Abstract]