Tumor Susceptibility Gene 101 (TSG101)

TSG10; VPS23; Tumor Susceptibility Gene 10; ESCRT-I complex subunit TSG101

SPN	U043
HGNC	15971
MGI	106581
PubMed	11208108
RGD	3909
UniProt	Q99816
Wiki	TSG101

Tumor susceptibility gene 101, also known as TSG101, is a protein belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis.

The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression.

Reference

"The TSG101 tumor susceptibility gene is located in chromosome 11 band p15 and is mutated in human breast cancer."
Cell 88:143-154(1997) [PubMed] [Europe PMC] [Abstract]

Erratum
Cell 93:661-661(1998) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Aberrant splicing of the TSG101 and FHIT genes occurs frequently in multiple malignancies and in normal tissues and mimics alterations previously described in tumours."
Oncogene 15:2119-2126(1997) [PubMed] [Europe PMC] [Abstract]

"Aberrant splicing but not mutations of TSG101 in human breast cancer."
Cancer Res. 57:3131-3134(1997) [PubMed] [Europe PMC] [Abstract]

"Genomic architecture and transcriptional activation of the mouse and human tumor susceptibility gene TSG101: common types of shorter transcripts are true alternative splice variants."
Oncogene 17:2761-2770(1998) [PubMed] [Europe PMC] [Abstract]

"DNMT1 binds HDAC2 and a new co-repressor, DMAP1, to form a complex at replication foci."
Nat. Genet. 25:269-277(2000) [PubMed] [Europe PMC] [Abstract]

"Tsg101 and the vacuolar protein sorting pathway are essential for HIV-1 budding."
Cell 107:55-65(2001) [PubMed] [Europe PMC] [Abstract]

"Tsg101, a homologue of ubiquitin-conjugating (E2) enzymes, binds the L domain in HIV type 1 Pr55(Gag)."
Proc. Natl. Acad. Sci. U.S.A. 98:7724-7729(2001) [PubMed] [Europe PMC] [Abstract]

"Mammalian class E vps proteins recognize ubiquitin and act in the removal of endosomal protein-ubiquitin conjugates."
J. Cell Biol. 157:91-101(2002) [PubMed] [Europe PMC] [Abstract]

"The protein network of HIV budding."
Cell 114:701-713(2003) [PubMed] [Europe PMC] [Abstract]

"HIV Gag mimics the Tsg101-recruiting activity of the human Hrs protein."
J. Cell Biol. 162:425-434(2003) [PubMed] [Europe PMC] [Abstract]

"Ebola virus matrix protein VP40 interaction with human cellular factors Tsg101 and Nedd4."
J. Mol. Biol. 326:493-502(2003) [PubMed] [Europe PMC] [Abstract]

"PPPYVEPTAP motif is the late domain of human T-cell leukemia virus type 1 Gag and mediates its functional interaction with cellular proteins Nedd4 and Tsg101."
J. Virol. 77:11882-11895(2003) [PubMed] [Europe PMC] [Abstract]

"TSG101 interaction with HRS mediates endosomal trafficking and receptor down-regulation."
Proc. Natl. Acad. Sci. U.S.A. 100:7626-7631(2003) [PubMed] [Europe PMC] [Abstract]

"Divergent retroviral late-budding domains recruit vacuolar protein sorting factors by using alternative adaptor proteins."
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Erratum
Proc. Natl. Acad. Sci. U.S.A. 100:152845-152845(2003)

"Tal, a Tsg101-specific E3 ubiquitin ligase, regulates receptor endocytosis and retrovirus budding."
Genes Dev. 18:1737-1752(2004) [PubMed] [Europe PMC] [Abstract]

"The human endosomal sorting complex required for transport (ESCRT-I) and its role in HIV-1 budding."
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"The trihelical bundle subdomain of the GGA proteins interacts with multiple partners through overlapping but distinct sites."
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"Interactions of GGA3 with the ubiquitin sorting machinery."
Nat. Cell Biol. 6:244-251(2004) [PubMed] [Europe PMC] [Abstract]

"Identification of human VPS37C, a component of endosomal sorting complex required for transport-I important for viral budding."
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"Identification of domains in gag important for prototypic foamy virus egress."
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"Cellular factors required for Lassa virus budding."
J. Virol. 80:4191-4195(2006) [PubMed] [Europe PMC] [Abstract]

"Identification of human MVB12 proteins as ESCRT-I subunits that function in HIV budding."
Cell Host Microbe 2:41-53(2007) [PubMed] [Europe PMC] [Abstract]

"Human ESCRT and ALIX proteins interact with proteins of the midbody and function in cytokinesis."
EMBO J. 26:4215-4227(2007) [PubMed] [Europe PMC] [Abstract]

"Spongiform neurodegeneration-associated E3 ligase Mahogunin ubiquitylates TSG101 and regulates endosomal trafficking."
Mol. Biol. Cell 18:1129-1142(2007) [PubMed] [Europe PMC] [Abstract]

"Parallels between cytokinesis and retroviral budding: a role for the ESCRT machinery."
Science 316:1908-1912(2007) [PubMed] [Europe PMC] [Abstract]

"Identification of Alix-type and non-Alix-type ALG-2-binding sites in human phospholipid scramblase 3: differential binding to an alternatively spliced isoform and amino acid-substituted mutants."
J. Biol. Chem. 283:9623-9632(2008) [PubMed] [Europe PMC] [Abstract]

"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]

"Penta-EF-hand protein ALG-2 functions as a Ca2+-dependent adaptor that bridges Alix and TSG101."
Biochem. Biophys. Res. Commun. 386:237-241(2009) [PubMed] [Europe PMC] [Abstract]

"Abnormal regulation of TSG101 in mice with spongiform neurodegeneration."
Biochim. Biophys. Acta 1792:1027-1035(2009) [PubMed] [Europe PMC] [Abstract]

"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

"UBAP1 is a component of an endosome-specific ESCRT-I complex that is essential for MVB sorting."
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"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]

"Structure and functional interactions of the Tsg101 UEV domain."
EMBO J. 21:2397-2406(2002) [PubMed] [Europe PMC] [Abstract]

"Structure of the Tsg101 UEV domain in complex with the PTAP motif of the HIV-1 p6 protein."
Nat. Struct. Biol. 9:812-817(2002) [PubMed] [Europe PMC] [Abstract]

"Ubiquitin recognition by the human TSG101 protein."
Mol. Cell 13:783-789(2004) [PubMed] [Europe PMC] [Abstract]

"Structure of human TSG101 UEV domain."
Acta Crystallogr. D 62:458-464(2006) [PubMed] [Europe PMC] [Abstract]

"Crystallographic and functional analysis of the ESCRT-I /HIV-1 Gag PTAP interaction."
Structure 18:1536-1547(2010) [PubMed] [Europe PMC] [Abstract]

"Elucidation of new binding interactions with the tumor susceptibility gene 101 (Tsg101) protein using modified HIV-1 Gag-p6 derived peptide ligands."
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