Nuclear Mitotic Apparatus Protein 1 (NUMA1)

NMP22; NUMA; Nuclear matrix protein-22; SP-H antigen

SPN	T948
HGNC	8059
MGI	2443665
PubMed	15896312
RGD	1308777
UniProt	Q14980
Wiki	NUMA1

The NUMA protein was one of the first to be described as a cell cycle-related protein based on a distinct immunofluorescent staining pattern: in interphase, NUMA is present throughout the nucleus, and in mitosis, it localizes to the spindle apparatus (Lydersen and Pettijohn, 1980). Some patients with autoimmune disease have antibodies directed against the NUMA protein. The full-length NUMA cDNA (Compton et al., 1992; Yang et al., 1992) predicts a protein with a calculated molecular mass of about 237 kD, containing 2 globular domains separated by a coiled-coil domain of 1,485 amino acids. Wong et al. (2006) stated that the coiled-coil region of NUMA can form parallel coiled-coil dimers. By fluorescence in situ hybridization, Sparks et al. (1993) demonstrated that the NUMA1 gene is present in single copy and located on chromosome 11q13.

Reference

"NuMA: an unusually long coiled-coil related protein in the mammalian nucleus."
J. Cell Biol. 116:1303-1317(1992) [PubMed] [Europe PMC] [Abstract]

"Primary structure of NuMA, an intranuclear protein that defines a novel pathway for segregation of proteins at mitosis."
J. Cell Biol. 116:1395-1408(1992) [PubMed] [Europe PMC] [Abstract]

"Primary structure and microtubule-interacting domain of the SP-H antigen: a mitotic map located at the spindle pole characterized as a homologous protein to NuMA."
J. Cell Sci. 105:589-600(1993) [PubMed] [Europe PMC] [Abstract]

"Human chromosome 11 DNA sequence and analysis including novel gene identification."
Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Nuclear proteins of the bovine esophageal epithelium. II. The NuMA gene gives rise to multiple mRNAs and gene products reactive with monoclonal antibody W1."
J. Cell Sci. 104:249-260(1993) [PubMed] [Europe PMC] [Abstract]

"Nuclear mitotic apparatus protein (NuMA): spindle association, nuclear targeting and differential subcellular localization of various NuMA isoforms."
J. Cell Sci. 107:1389-1402(1994) [PubMed] [Europe PMC] [Abstract]

"Mutation of the predicted p34cdc2 phosphorylation sites in NuMA impair the assembly of the mitotic spindle and block mitosis."
J. Cell Sci. 108:621-633(1995) [PubMed] [Europe PMC] [Abstract]

"Sensitivity and specificity of NMP-22, telomerase, and BTA in the detection of human bladder cancer."
Urology 52:398-402(1998) [PubMed] [Europe PMC] [Abstract]

"Direct binding of NuMA to tubulin is mediated by a novel sequence motif in the tail domain that bundles and stabilizes microtubules."
J. Cell Sci. 115:1815-1824(2002) [PubMed] [Europe PMC] [Abstract]

"NuMA is a major acceptor of poly(ADP-ribosyl)ation by tankyrase 1 in mitosis."
Biochem. J. 391:177-184(2005) [PubMed] [Europe PMC] [Abstract]

"Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]

"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]

"A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]

"Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra."
J. Proteome Res. 6:4150-4162(2007) [PubMed] [Europe PMC] [Abstract]

"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."
Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]

"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]

"Phosphorylation analysis of primary human T lymphocytes using sequential IMAC and titanium oxide enrichment."
J. Proteome Res. 7:5167-5176(2008) [PubMed] [Europe PMC] [Abstract]

"A quantitative atlas of mitotic phosphorylation."
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]

"Requirements for NuMA in maintenance and establishment of mammalian spindle poles."
J. Cell Biol. 184:677-690(2009) [PubMed] [Europe PMC] [Abstract]

"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]

"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]

"Extensive crosstalk between O-GlcNAcylation and phosphorylation regulates cytokinesis."
Sci. Signal. 3:RA2-RA2(2010) [PubMed] [Europe PMC] [Abstract]

"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]

"NuMA after 30 years: the matrix revisited."
Trends Cell Biol. 20:214-222(2010) [PubMed] [Europe PMC] [Abstract]

"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]

"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]

"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]

"Self assembly of NuMA: multiarm oligomers as structural units of a nuclear lattice."
EMBO J. 18:1689-1700(1999) [PubMed] [Europe PMC] [Abstract]