Insulin Like Growth Factor Binding Protein 5 (IGFBP5)

IBP5

SPN	T874
HGNC	5474
MGI	96440
PubMed	16700616
RGD	2876
UniProt	P24593
Wiki	IGFBP5

Insulin-like growth factor-binding protein 5 is a protein encoded by the IGFBP5 gene. Allander et al. (1994) showed that the IGFBP5 gene is divided into 4 exons and spans about 33 kb, primarily due to a first intron of approximately 25 kb. Primer extension studies identified the IGFBP5 mRNA cap site 772 bp 5-prime to the first nucleotide of the translation start codon. A potential TATA element beginning 33 bp 5-prime to the mRNA cap site was identified. When a DNA fragment containing this cap site and 461 bp of upstream sequence was placed 5-prime to the chloramphenicol acetyltransferase (CAT) reporter gene and transfected into human breast cancer cells, it directed CAT expression in an orientation-specific manner, suggesting that this region contains elements essential for IGFBP5 promoter activity.

Reference

"Molecular cloning of a new human insulin-like growth factor binding protein."
Biochem. Biophys. Res. Commun. 176:219-225(1991) [PubMed] [Europe PMC] [Abstract]

"Identification of five different insulin-like growth factor binding proteins (IGFBPs) from adult rat serum and molecular cloning of a novel IGFBP-5 in rat and human."
J. Biol. Chem. 266:10646-10653(1991) [PubMed] [Europe PMC] [Abstract]

"Characterization of the chromosomal gene and promoter for human insulin-like growth factor binding protein-5."
J. Biol. Chem. 269:10891-10898(1994) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"A novel human insulin-like growth factor binding protein secreted by osteoblast-like cells."
Biochem. Biophys. Res. Commun. 176:213-218(1991) [PubMed] [Europe PMC] [Abstract]

"Isolation and characterization of circulating 13-kDa C-terminal fragments of human insulin-like growth factor binding protein-5."
FEBS Lett. 441:281-286(1998) [PubMed] [Europe PMC] [Abstract]

"Defining the disulfide bonds of insulin-like growth factor-binding protein-5 by tandem mass spectrometry with electron transfer dissociation and collision-induced dissociation."
J. Biol. Chem. 287:1510-1519(2012) [PubMed] [Europe PMC] [Abstract]

"Structure of the IGF-binding domain of the insulin-like growth factor-binding protein-5 (IGFBP-5): implications for IGF and IGF-I receptor interactions."
EMBO J. 17:6558-6572(1998) [PubMed] [Europe PMC] [Abstract]