Cathepsin A (CTSA)
CTS-A; GLB2; GSL; NGBE; PPCA; PPGB; Protective Protein For Beta-Galactosidase; Galactosialidosis; Carboxypeptidase C; Carboxypeptidase L
Cathepsin A is a ubiquitously expressed multifunctional enzyme, with deamidase, esterase, and carboxypeptidase activities and a preference for substrates with hydrophobic amino acid residues at the P1-prime position. Association with CTSA, as part of the lysosomal multienzyme complex, is essential for stabilization of lysosomal beta-galactosidase (GLB1), as well as for activation of the lysosomal neuraminidase (NEU1) . Galjart et al. isolated cDNA encoding the human 'protective protein.' The cDNA directed the synthesis of a 452-amino acid precursor molecule that is processed in vivo to yield mature 'protective protein,' a heterodimer of 32 kD and 20 kD polypeptides held together by disulfide bridges. Because of homology to yeast carboxypeptidase Y, Galjart et al. suggested a protease activity for the 'protective protein.'
Organism species: Homo sapiens (Human)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | NONE | Recombinant Cathepsin A (CTSA) | Positive Control; Immunogen; SDS-PAGE; WB. |
| NONE | Native Cathepsin A (CTSA) | Bioactivity | |
| Antibodies | MT841Hu21 | Monoclonal Antibody to Cathepsin A (CTSA) | WB; IHC; ICC; IP. |
| PT841Hu01 | Polyclonal Antibody to Cathepsin A (CTSA) | WB; IHC; ICC; IF. | |
| LT841Hu71 | Biotin Linked-Polyclonal Antibody to Cathepsin A (CTSA) | ELISA(Det);IC; IH; IH(P); WB | |
| NONE | APC Linked-Polyclonal Antibody to Cathepsin A (CTSA) | IHC, WB | |
| NONE | HRP Linked-Polyclonal Antibody to Cathepsin A (CTSA) | Simple Western; WB | |
| NONE | PE Linked-Polyclonal Antibody to Cathepsin A (CTSA) | Flow Cytometry | |
| LT841Hu81 | FITC Linked-Polyclonal Antibody to Cathepsin A (CTSA) | Flow Cytometry;ICC/IF | |
| Assay Kits | NONE | ELISA Kit for Cathepsin A (CTSA) | Enzyme-linked immunosorbent assay for Antigen Detection |
| NONE | CLIA kit for Cathepsin A (CTSA) | CLIA assay for Antigen Detection |
Organism species: Mus musculus (Mouse)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | T841Mu02 | Recombinant Cathepsin A (CTSA) | Positive Control; Immunogen; SDS-PAGE; WB. |
| NONE | Native Cathepsin A (CTSA) | Bioactivity | |
| Antibodies | NONE | Monoclonal Antibody to Cathepsin A (CTSA) | WB; IHC; ICC; IP. |
| PT841Mu01 | Polyclonal Antibody to Cathepsin A (CTSA) | WB; IHC; ICC; IF. | |
| LT841Mu71 | Biotin Linked-Polyclonal Antibody to Cathepsin A (CTSA) | ELISA(Det);IC; IH; IH(P); WB | |
| NONE | APC Linked-Polyclonal Antibody to Cathepsin A (CTSA) | IHC, WB | |
| NONE | HRP Linked-Polyclonal Antibody to Cathepsin A (CTSA) | Simple Western; WB | |
| NONE | PE Linked-Polyclonal Antibody to Cathepsin A (CTSA) | Flow Cytometry | |
| LT841Mu81 | FITC Linked-Polyclonal Antibody to Cathepsin A (CTSA) | Flow Cytometry;ICC/IF | |
| Assay Kits | KET841Mu | ELISA Kit for Cathepsin A (CTSA) | Enzyme-linked immunosorbent assay for Antigen Detection |
| NONE | CLIA kit for Cathepsin A (CTSA) | CLIA assay for Antigen Detection |
Organism species: Rattus norvegicus (Rat)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | NONE | Recombinant Cathepsin A (CTSA) | Positive Control; Immunogen; SDS-PAGE; WB. |
| NONE | Native Cathepsin A (CTSA) | Bioactivity | |
| Antibodies | NONE | Monoclonal Antibody to Cathepsin A (CTSA) | WB; IHC; ICC; IP. |
| PT841Ra01 | Polyclonal Antibody to Cathepsin A (CTSA) | WB; IHC; ICC; IF. | |
| LT841Ra71 | Biotin Linked-Polyclonal Antibody to Cathepsin A (CTSA) | ELISA(Det);IC; IH; IH(P); WB | |
| NONE | APC Linked-Polyclonal Antibody to Cathepsin A (CTSA) | IHC, WB | |
| NONE | HRP Linked-Polyclonal Antibody to Cathepsin A (CTSA) | Simple Western; WB | |
| NONE | PE Linked-Polyclonal Antibody to Cathepsin A (CTSA) | Flow Cytometry | |
| LT841Ra81 | FITC Linked-Polyclonal Antibody to Cathepsin A (CTSA) | Flow Cytometry;ICC/IF | |
| Assay Kits | NONE | ELISA Kit for Cathepsin A (CTSA) | Enzyme-linked immunosorbent assay for Antigen Detection |
| NONE | CLIA kit for Cathepsin A (CTSA) | CLIA assay for Antigen Detection |
Organism species: Canis familiaris; Canine (Dog)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | NONE | Recombinant Cathepsin A (CTSA) | Positive Control; Immunogen; SDS-PAGE; WB. |
| NONE | Native Cathepsin A (CTSA) | Bioactivity | |
| Antibodies | NONE | Monoclonal Antibody to Cathepsin A (CTSA) | WB; IHC; ICC; IP. |
| PT841Ca01 | Polyclonal Antibody to Cathepsin A (CTSA) | WB; IHC; ICC; IF. | |
| LT841Ca71 | Biotin Linked-Polyclonal Antibody to Cathepsin A (CTSA) | ELISA(Det);IC; IH; IH(P); WB | |
| NONE | APC Linked-Polyclonal Antibody to Cathepsin A (CTSA) | IHC, WB | |
| NONE | HRP Linked-Polyclonal Antibody to Cathepsin A (CTSA) | Simple Western; WB | |
| NONE | PE Linked-Polyclonal Antibody to Cathepsin A (CTSA) | Flow Cytometry | |
| LT841Ca81 | FITC Linked-Polyclonal Antibody to Cathepsin A (CTSA) | Flow Cytometry;ICC/IF | |
| Assay Kits | NONE | ELISA Kit for Cathepsin A (CTSA) | Enzyme-linked immunosorbent assay for Antigen Detection |
| NONE | CLIA kit for Cathepsin A (CTSA) | CLIA assay for Antigen Detection |
Organism species: Sus scrofa; Porcine (Pig)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | NONE | Recombinant Cathepsin A (CTSA) | Positive Control; Immunogen; SDS-PAGE; WB. |
| NONE | Native Cathepsin A (CTSA) | Bioactivity | |
| Antibodies | NONE | Monoclonal Antibody to Cathepsin A (CTSA) | WB; IHC; ICC; IP. |
| PT841Po01 | Polyclonal Antibody to Cathepsin A (CTSA) | WB; IHC; ICC; IF. | |
| LT841Po71 | Biotin Linked-Polyclonal Antibody to Cathepsin A (CTSA) | ELISA(Det);IC; IH; IH(P); WB | |
| NONE | APC Linked-Polyclonal Antibody to Cathepsin A (CTSA) | IHC, WB | |
| NONE | HRP Linked-Polyclonal Antibody to Cathepsin A (CTSA) | Simple Western; WB | |
| NONE | PE Linked-Polyclonal Antibody to Cathepsin A (CTSA) | Flow Cytometry | |
| LT841Po81 | FITC Linked-Polyclonal Antibody to Cathepsin A (CTSA) | Flow Cytometry;ICC/IF | |
| Assay Kits | NONE | ELISA Kit for Cathepsin A (CTSA) | Enzyme-linked immunosorbent assay for Antigen Detection |
| NONE | CLIA kit for Cathepsin A (CTSA) | CLIA assay for Antigen Detection |
Organism species: Bos taurus; Bovine (Cattle)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | T841Bo01 | Recombinant Cathepsin A (CTSA) | Positive Control; Immunogen; SDS-PAGE; WB. |
| NONE | Native Cathepsin A (CTSA) | Bioactivity | |
| Antibodies | NONE | Monoclonal Antibody to Cathepsin A (CTSA) | WB; IHC; ICC; IP. |
| PT841Bo01 | Polyclonal Antibody to Cathepsin A (CTSA) | WB; IHC; ICC; IF. | |
| NONE | Biotin Linked-Polyclonal Antibody to Cathepsin A (CTSA) | ELISA(Det);IC; IH; IH(P); WB | |
| NONE | APC Linked-Polyclonal Antibody to Cathepsin A (CTSA) | IHC, WB | |
| NONE | HRP Linked-Polyclonal Antibody to Cathepsin A (CTSA) | Simple Western; WB | |
| NONE | PE Linked-Polyclonal Antibody to Cathepsin A (CTSA) | Flow Cytometry | |
| NONE | FITC Linked-Polyclonal Antibody to Cathepsin A (CTSA) | Flow Cytometry;ICC/IF | |
| Assay Kits | NONE | ELISA Kit for Cathepsin A (CTSA) | Enzyme-linked immunosorbent assay for Antigen Detection |
| NONE | CLIA kit for Cathepsin A (CTSA) | CLIA assay for Antigen Detection |
Organism species: Rhesus monkey (Simian)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | NONE | Recombinant Cathepsin A (CTSA) | Positive Control; Immunogen; SDS-PAGE; WB. |
| NONE | Native Cathepsin A (CTSA) | Bioactivity | |
| Antibodies | NONE | Monoclonal Antibody to Cathepsin A (CTSA) | WB; IHC; ICC; IP. |
| NONE | Polyclonal Antibody to Cathepsin A (CTSA) | WB; IHC; ICC; IF. | |
| NONE | Biotin Linked-Polyclonal Antibody to Cathepsin A (CTSA) | ELISA(Det);IC; IH; IH(P); WB | |
| NONE | APC Linked-Polyclonal Antibody to Cathepsin A (CTSA) | IHC, WB | |
| NONE | HRP Linked-Polyclonal Antibody to Cathepsin A (CTSA) | Simple Western; WB | |
| NONE | PE Linked-Polyclonal Antibody to Cathepsin A (CTSA) | Flow Cytometry | |
| NONE | FITC Linked-Polyclonal Antibody to Cathepsin A (CTSA) | Flow Cytometry;ICC/IF | |
| Assay Kits | NONE | ELISA Kit for Cathepsin A (CTSA) | Enzyme-linked immunosorbent assay for Antigen Detection |
| NONE | CLIA kit for Cathepsin A (CTSA) | CLIA assay for Antigen Detection |
Organism species: Chicken (Gallus)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | NONE | Recombinant Cathepsin A (CTSA) | Positive Control; Immunogen; SDS-PAGE; WB. |
| NONE | Native Cathepsin A (CTSA) | Bioactivity | |
| Antibodies | NONE | Monoclonal Antibody to Cathepsin A (CTSA) | WB; IHC; ICC; IP. |
| NONE | Polyclonal Antibody to Cathepsin A (CTSA) | WB; IHC; ICC; IF. | |
| NONE | Biotin Linked-Polyclonal Antibody to Cathepsin A (CTSA) | ELISA(Det);IC; IH; IH(P); WB | |
| NONE | APC Linked-Polyclonal Antibody to Cathepsin A (CTSA) | IHC, WB | |
| NONE | HRP Linked-Polyclonal Antibody to Cathepsin A (CTSA) | Simple Western; WB | |
| NONE | PE Linked-Polyclonal Antibody to Cathepsin A (CTSA) | Flow Cytometry | |
| NONE | FITC Linked-Polyclonal Antibody to Cathepsin A (CTSA) | Flow Cytometry;ICC/IF | |
| Assay Kits | NONE | ELISA Kit for Cathepsin A (CTSA) | Enzyme-linked immunosorbent assay for Antigen Detection |
| NONE | CLIA kit for Cathepsin A (CTSA) | CLIA assay for Antigen Detection |
Organism species: Ovis aries; Ovine (Sheep)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | NONE | Recombinant Cathepsin A (CTSA) | Positive Control; Immunogen; SDS-PAGE; WB. |
| NONE | Native Cathepsin A (CTSA) | Bioactivity | |
| Antibodies | NONE | Monoclonal Antibody to Cathepsin A (CTSA) | WB; IHC; ICC; IP. |
| NONE | Polyclonal Antibody to Cathepsin A (CTSA) | WB; IHC; ICC; IF. | |
| NONE | Biotin Linked-Polyclonal Antibody to Cathepsin A (CTSA) | ELISA(Det);IC; IH; IH(P); WB | |
| NONE | APC Linked-Polyclonal Antibody to Cathepsin A (CTSA) | IHC, WB | |
| NONE | HRP Linked-Polyclonal Antibody to Cathepsin A (CTSA) | Simple Western; WB | |
| NONE | PE Linked-Polyclonal Antibody to Cathepsin A (CTSA) | Flow Cytometry | |
| NONE | FITC Linked-Polyclonal Antibody to Cathepsin A (CTSA) | Flow Cytometry;ICC/IF | |
| Assay Kits | NONE | ELISA Kit for Cathepsin A (CTSA) | Enzyme-linked immunosorbent assay for Antigen Detection |
| NONE | CLIA kit for Cathepsin A (CTSA) | CLIA assay for Antigen Detection |
Organism species: Oryctolagus cuniculus (Rabbit)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | NONE | Recombinant Cathepsin A (CTSA) | Positive Control; Immunogen; SDS-PAGE; WB. |
| NONE | Native Cathepsin A (CTSA) | Bioactivity | |
| Antibodies | NONE | Monoclonal Antibody to Cathepsin A (CTSA) | WB; IHC; ICC; IP. |
| NONE | Polyclonal Antibody to Cathepsin A (CTSA) | WB; IHC; ICC; IF. | |
| NONE | Biotin Linked-Polyclonal Antibody to Cathepsin A (CTSA) | ELISA(Det);IC; IH; IH(P); WB | |
| NONE | APC Linked-Polyclonal Antibody to Cathepsin A (CTSA) | IHC, WB | |
| NONE | HRP Linked-Polyclonal Antibody to Cathepsin A (CTSA) | Simple Western; WB | |
| NONE | PE Linked-Polyclonal Antibody to Cathepsin A (CTSA) | Flow Cytometry | |
| NONE | FITC Linked-Polyclonal Antibody to Cathepsin A (CTSA) | Flow Cytometry;ICC/IF | |
| Assay Kits | NONE | ELISA Kit for Cathepsin A (CTSA) | Enzyme-linked immunosorbent assay for Antigen Detection |
| NONE | CLIA kit for Cathepsin A (CTSA) | CLIA assay for Antigen Detection |
Organism species: Cavia (Guinea pig )
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | NONE | Recombinant Cathepsin A (CTSA) | Positive Control; Immunogen; SDS-PAGE; WB. |
| NONE | Native Cathepsin A (CTSA) | Bioactivity | |
| Antibodies | NONE | Monoclonal Antibody to Cathepsin A (CTSA) | WB; IHC; ICC; IP. |
| NONE | Polyclonal Antibody to Cathepsin A (CTSA) | WB; IHC; ICC; IF. | |
| NONE | Biotin Linked-Polyclonal Antibody to Cathepsin A (CTSA) | ELISA(Det);IC; IH; IH(P); WB | |
| NONE | APC Linked-Polyclonal Antibody to Cathepsin A (CTSA) | IHC, WB | |
| NONE | HRP Linked-Polyclonal Antibody to Cathepsin A (CTSA) | Simple Western; WB | |
| NONE | PE Linked-Polyclonal Antibody to Cathepsin A (CTSA) | Flow Cytometry | |
| NONE | FITC Linked-Polyclonal Antibody to Cathepsin A (CTSA) | Flow Cytometry;ICC/IF | |
| Assay Kits | NONE | ELISA Kit for Cathepsin A (CTSA) | Enzyme-linked immunosorbent assay for Antigen Detection |
| NONE | CLIA kit for Cathepsin A (CTSA) | CLIA assay for Antigen Detection |
Organism species: Equus caballus; Equine (Horse)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | NONE | Recombinant Cathepsin A (CTSA) | Positive Control; Immunogen; SDS-PAGE; WB. |
| NONE | Native Cathepsin A (CTSA) | Bioactivity | |
| Antibodies | NONE | Monoclonal Antibody to Cathepsin A (CTSA) | WB; IHC; ICC; IP. |
| NONE | Polyclonal Antibody to Cathepsin A (CTSA) | WB; IHC; ICC; IF. | |
| NONE | Biotin Linked-Polyclonal Antibody to Cathepsin A (CTSA) | ELISA(Det);IC; IH; IH(P); WB | |
| NONE | APC Linked-Polyclonal Antibody to Cathepsin A (CTSA) | IHC, WB | |
| NONE | HRP Linked-Polyclonal Antibody to Cathepsin A (CTSA) | Simple Western; WB | |
| NONE | PE Linked-Polyclonal Antibody to Cathepsin A (CTSA) | Flow Cytometry | |
| NONE | FITC Linked-Polyclonal Antibody to Cathepsin A (CTSA) | Flow Cytometry;ICC/IF | |
| Assay Kits | NONE | ELISA Kit for Cathepsin A (CTSA) | Enzyme-linked immunosorbent assay for Antigen Detection |
| NONE | CLIA kit for Cathepsin A (CTSA) | CLIA assay for Antigen Detection |
Organism species: Capra hircus; Caprine (Goat)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | NONE | Recombinant Cathepsin A (CTSA) | Positive Control; Immunogen; SDS-PAGE; WB. |
| NONE | Native Cathepsin A (CTSA) | Bioactivity | |
| Antibodies | NONE | Monoclonal Antibody to Cathepsin A (CTSA) | WB; IHC; ICC; IP. |
| NONE | Polyclonal Antibody to Cathepsin A (CTSA) | WB; IHC; ICC; IF. | |
| NONE | Biotin Linked-Polyclonal Antibody to Cathepsin A (CTSA) | ELISA(Det);IC; IH; IH(P); WB | |
| NONE | APC Linked-Polyclonal Antibody to Cathepsin A (CTSA) | IHC, WB | |
| NONE | HRP Linked-Polyclonal Antibody to Cathepsin A (CTSA) | Simple Western; WB | |
| NONE | PE Linked-Polyclonal Antibody to Cathepsin A (CTSA) | Flow Cytometry | |
| NONE | FITC Linked-Polyclonal Antibody to Cathepsin A (CTSA) | Flow Cytometry;ICC/IF | |
| Assay Kits | NONE | ELISA Kit for Cathepsin A (CTSA) | Enzyme-linked immunosorbent assay for Antigen Detection |
| NONE | CLIA kit for Cathepsin A (CTSA) | CLIA assay for Antigen Detection |
Organism species: Pan-species (General)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | NONE | Recombinant Cathepsin A (CTSA) | Positive Control; Immunogen; SDS-PAGE; WB. |
| NONE | Native Cathepsin A (CTSA) | Bioactivity | |
| Antibodies | NONE | Monoclonal Antibody to Cathepsin A (CTSA) | WB; IHC; ICC; IP. |
| NONE | Polyclonal Antibody to Cathepsin A (CTSA) | WB; IHC; ICC; IF. | |
| NONE | Biotin Linked-Polyclonal Antibody to Cathepsin A (CTSA) | ELISA(Det);IC; IH; IH(P); WB | |
| NONE | APC Linked-Polyclonal Antibody to Cathepsin A (CTSA) | IHC, WB | |
| NONE | HRP Linked-Polyclonal Antibody to Cathepsin A (CTSA) | Simple Western; WB | |
| NONE | PE Linked-Polyclonal Antibody to Cathepsin A (CTSA) | Flow Cytometry | |
| NONE | FITC Linked-Polyclonal Antibody to Cathepsin A (CTSA) | Flow Cytometry;ICC/IF | |
| Assay Kits | NONE | ELISA Kit for Cathepsin A (CTSA) | Enzyme-linked immunosorbent assay for Antigen Detection |
| NONE | CLIA kit for Cathepsin A (CTSA) | CLIA assay for Antigen Detection |
Reference
"Expression of cDNA encoding the human 'protective protein' associated with lysosomal beta-galactosidase and neuraminidase: homology to yeast proteases."
Cell 54:755-764(1988) [PubMed] [Europe PMC] [Abstract]
"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
"The DNA sequence and comparative analysis of human chromosome 20."
Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
"A peptidase in human platelets that deamidates tachykinins. Probable identity with the lysosomal 'protective protein'."
J. Biol. Chem. 265:11265-11272(1990) [PubMed] [Europe PMC] [Abstract]
"Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides."
Nat. Biotechnol. 21:566-569(2003) [PubMed] [Europe PMC] [Abstract]
"Human lysosomal protective protein has cathepsin A-like activity distinct from its protective function."
J. Biol. Chem. 266:14754-14762(1991) [PubMed] [Europe PMC] [Abstract]
"Elucidation of N-glycosylation sites on human platelet proteins: a glycoproteomic approach."
Mol. Cell. Proteomics 5:226-233(2006) [PubMed] [Europe PMC] [Abstract]
"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
"Three-dimensional structure of the human 'protective protein': structure of the precursor form suggests a complex activation mechanism."
Structure 3:1249-1259(1995) [PubMed] [Europe PMC] [Abstract]
"A mutation in a mild form of galactosialidosis impairs dimerization of the protective protein and renders it unstable."
EMBO J. 10:4041-4048(1991) [PubMed] [Europe PMC] [Abstract]
"Protective protein gene mutations in galactosialidosis."
J. Clin. Invest. 91:2393-2398(1993) [PubMed] [Europe PMC] [Abstract]
"Molecular and biochemical analysis of protective protein/cathepsin A mutations: correlation with clinical severity in galactosialidosis."
Hum. Mol. Genet. 5:1977-1987(1996) [PubMed] [Europe PMC] [Abstract]
"Structural and functional study of K453E mutant protective protein/cathepsin A causing the late infantile form of galactosialidosis."
J. Hum. Genet. 45:200-206(2000) [PubMed] [Europe PMC] [Abstract]