Nucleobindin 2 (NUCB2)

NUCB2; NEFA; Nucleobindin 2; DNA-binding protein NEFA; Gastric cancer antigen Zg4; Prepronesfatin

SPN	T602
HGNC	8044
MGI	1858179
PubMed	24478398
RGD	620888
UniProt	P80303
Wiki	NUCB2

Barnikol-Watanabe et al. (1994) isolated a partial NUCB2 cDNA clone. They assembled a full-length clone encoding a deduced 420-amino acid protein, which they called NEFA, with a calculated molecular mass of 48 kD. The protein contains a basic amino acid-rich putative DNA-binding domain with a potential nuclear targeting signal, 2 helix-loop-helix motif regions, 2 concurrent EF-hand motifs, an acidic amino acid region between the EF-hands, and a leucine zipper motif. The protein shares high homology with human NUCB1. Isoelectric focusing demonstrated the presence of NUCB2 on the plasma membrane, in the cytosol, and in the culture supernatant. NUCB2 is a highly charged protein with a relative molecular mass of 55 kD by SDS-PAGE, despite the lack of N- or O-glycosylation sites.

Reference

"Human protein NEFA, a novel DNA binding/EF-hand/leucine zipper protein. Molecular cloning and sequence analysis of the cDNA, isolation and characterization of the protein."
Biol. Chem. Hoppe-Seyler 375:497-512(1994) [PubMed] [Europe PMC] [Abstract]

"Assembly of a high-resolution map of the Acadian Usher syndrome region and localization of the nuclear EF-hand acidic gene."
Biochim. Biophys. Acta 1407:84-91(1998) [PubMed] [Europe PMC] [Abstract]

"Serological identification and expression analysis of gastric cancer-associated genes."
Br. J. Cancer 86:1824-1830(2002) [PubMed] [Europe PMC] [Abstract]

"Troglitazone, a ligand of peroxisome proliferator-activated receptor-gamma, stabilizes NUCB2 (Nesfatin) mRNA by activating the ERK1/2 pathway: isolation and characterization of the human NUCB2 gene."
Endocrinology 151:2494-2503(2010) [PubMed] [Europe PMC] [Abstract]

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

"Human chromosome 11 DNA sequence and analysis including novel gene identification."
Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract]

"Golgi retention of human protein NEFA is mediated by its N-terminal Leu/Ile-rich region."
FEBS Lett. 509:469-475(2001) [PubMed] [Europe PMC] [Abstract]

"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]

"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]

"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]

"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]