Leukocyte Elastase Inhibitor (LEI)

SERPINB1; EI; ELANH2; LEI; M/NEI; MNEI; PI2; Serpin Peptidase Inhibitor Clade B Member 1; Anti-Elastase; Monocyte/Neutrophil Elastase Inhibitor

SPN	T394
HGNC	3311
MGI	1913472
PubMed	19723034
UniProt	P30740
Wiki	SERPINB1

Monocyte/neutrophil elastase inhibitor (EI) is a protein of approximately 42,000 Mr with serpin-like functional properties. Remold-O'Donnell et al. (1992) cloned EI cDNA and identified 3 EI mRNA species of 1.5, 1.9, and 2.6 kb in monocyte-like cells and no hybridizing mRNA in lymphoblastoid cells lacking detectable EI enzymatic activity. The cDNA open reading frame encoded a 379-amino acid protein. Its sequence established EI as a member of the serpin superfamily. Sequence alignment indicated that the reactive center P1 residue is cys-344, consistent with abrogation of elastase inhibitory activity by iodoacetamide and making EI a naturally occurring cys-serpin. Other elastase inhibitors include alpha-1-antitrypsin (PI) and secretory leukocyte protease inhibitor (SLPI).

Reference

"Sequence and molecular characterization of human monocyte/neutrophil elastase inhibitor."
Proc. Natl. Acad. Sci. U.S.A. 89:5635-5639(1992) [PubMed] [Europe PMC] [Abstract]

"Structure and sequence of human M/NEI (monocyte/neutrophil elastase inhibitor), an Ov-serpin family gene."
Gene 213:179-187(1998) [PubMed] [Europe PMC] [Abstract]

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

"The DNA sequence and analysis of human chromosome 6."
Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides."
Nat. Biotechnol. 21:566-569(2003) [PubMed] [Europe PMC] [Abstract]

"A serpin from human tumor cells with direct lymphoid immunomodulatory activity: mitogenic stimulation of human tumor-infiltrating lymphocytes."
Biochim. Biophys. Acta 1269:41-50(1995) [PubMed] [Europe PMC] [Abstract]

"Microsequences of 145 proteins recorded in the two-dimensional gel protein database of normal human epidermal keratinocytes."
Electrophoresis 13:960-969(1992) [PubMed] [Europe PMC] [Abstract]

"The serpin MNEI inhibits elastase-like and chymotrypsin-like serine proteases through efficient reactions at two active sites."
Biochemistry 40:15762-15770(2001) [PubMed] [Europe PMC] [Abstract]

"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]

"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]

"Identification of SERPINB1 as a physiological inhibitor of human granzyme H."
J. Immunol. 190:1319-1330(2013) [PubMed] [Europe PMC] [Abstract]