Trypsin (TRY)

SPN	T316
PubMed	15034119
UniProt	P00761
Wiki	Trypsin

Trypsin is produced in the pancreas as the inactive proenzyme trypsinogen. Trypsin predominantly cleaves peptide chains at the carboxyl side of the amino acids lysine or arginine, except when either is followed by proline. It is used for numerous biotechnological processes. The process is commonly referred to as trypsin proteolysis or trypsinisation and proteins that have been digested/treated with trypsin are said to have been trypsinized.Trypsin is secreted into the duodenum, where it acts to hydrolyse peptides into their smaller building blocks, namely amino acids (these peptides are the result of the enzyme pepsin breaking down the proteins in the stomach). This is necessary for the uptake of protein in the food as though peptides are smaller than proteins, they are still too big to be absorbed through the lining of the ileum. Trypsin catalyses the hydrolysis of peptide bonds.

Reference

"On trypsinogen and trypsin of pig."
Biochim. Biophys. Acta 69:115-129(1963) [PubMed] [Europe PMC] [Abstract]

"Determination of the amino acid sequence of porcine trypsin by sequenator analysis."
Biochemistry 12:3146-3153(1973) [PubMed] [Europe PMC] [Abstract]

"Refined 1.6-A resolution crystal structure of the complex formed between porcine beta-trypsin and MCTI-A, a trypsin inhibitor of the squash family. Detailed comparison with bovine beta-trypsin and its complex."
J. Mol. Biol. 229:1022-1036(1993) [PubMed] [Europe PMC] [Abstract]

"Amino acid sequencing of a trypsin inhibitor by refined 1.6 A X-ray crystal structure of its complex with porcine beta-trypsin."
FEBS Lett. 297:143-146(1992) [PubMed] [Europe PMC] [Abstract]

"Refined 1.8-A resolution crystal structure of the porcine epsilon-trypsin."
Biochim. Biophys. Acta 1209:77-82(1994) [PubMed] [Europe PMC] [Abstract]

"The three-dimensional structure of recombinant leech-derived tryptase inhibitor in complex with trypsin. Implications for the structure of human mast cell tryptase and its inhibition."
J. Biol. Chem. 272:19931-19937(1997) [PubMed] [Europe PMC] [Abstract]

"Structure of the complex of leech-derived tryptase inhibitor (LDTI) with trypsin and modeling of the LDTI-tryptase system."
Structure 5:1465-1474(1997) [PubMed] [Europe PMC] [Abstract]

"L-isoaspartate 115 of porcine beta-trypsin promotes crystallization of its complex with bdellastasin."
Acta Crystallogr. D 56:581-588(2000) [PubMed] [Europe PMC] [Abstract]