Acyl Coenzyme A Synthetase Medium Chain Family, Member 1 (ACSM1)

MACS1; BUCS1; LAE; Butyryl Coenzyme A Synthetase 1; Acyl-CoA synthetase medium-chain family member 1; Middle-chain acyl-CoA synthetase 1; Lipoate-activating enzyme

SPN	T115
HGNC	18049
MGI	2152200
PubMed	18632593
UniProt	Q08AH1

ACSM1 has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). Acts on acids from C₄ to C(11) and on the corresponding 3-hydroxy- and 2,3- or 3,4-unsaturated acids (in vitro). Functions as GTP-dependent lipoate-activating enzyme that generates the substrate for lipoyltransferase.

The medium chain acyl-CoA dehydrogenase is the best known structure of all acyl-CoA dehydrogenases, and is the most commonly deficient enzyme within the class that leads to metabolic disorders in animals. The molecule is a homotetramer with each subunit containing roughly 400 amino acids and one equivalent of FAD. The tetramer is classified as a “dimer of dimers” with an overall diameter of approximately 90 Å.

Reference

"Molecular identification and characterization of two medium-chain acyl-CoA synthetases, MACS1 and the Sa gene product."
J. Biol. Chem. 276:35961-35966(2001) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]