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Runt Related Transcription Factor 1 (RUNX1)

AML1; AMLCR1; CBFA2; EVI-1; PEBP2aB; Acute Myeloid Leukemia 1; Aml1 Oncogene; Core-binding factor subunit alpha-2; Polyomavirus enhancer-binding protein 2 alpha B subunit

RUNX1 is an eukaryotic gene and the protein encoded by this gene is a transcription factor associated with M2 AML, a type of leukemia. It belongs to the Runt-related transcription factor (RUNX) family of genes which are also called core binding factor-α (CBFα). RUNX proteins form a heterodimeric complex with CBFβ which confers increased DNA binding and stability to the complex. RUNX1 is thought to be involved in the development of normal hematopoiesis. Chromosomal translocations involving this gene are well-documented and have been associated with several types of leukemia. Two transcript variants encoding different isoforms have been found for this gene. The consensus binding site for CBF has been identified to be a 7 bp sequence PyGPyGGTPy. Py denotes pyrimidine which can be either cytosine or thymine.

Organism species: Homo sapiens (Human)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPT035Hu01 Recombinant Runt Related Transcription Factor 1 (RUNX1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAT035Hu01 Monoclonal Antibody to Runt Related Transcription Factor 1 (RUNX1) WB; IHC; ICC; IP.
PAT035Hu01 Polyclonal Antibody to Runt Related Transcription Factor 1 (RUNX1) WB; IHC; ICC; IF.
Assay Kits CLT035Hu01 CLIA Kit for Runt Related Transcription Factor 1 (RUNX1) CLIA assay for Antigen Detection
ELT035Hu01 ELISA Kit for Runt Related Transcription Factor 1 (RUNX1) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Mus musculus (Mouse)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPT035Mu01 Recombinant Runt Related Transcription Factor 1 (RUNX1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAT035Mu01 Monoclonal Antibody to Runt Related Transcription Factor 1 (RUNX1) WB; IHC; ICC; IP.
PAT035Mu01 Polyclonal Antibody to Runt Related Transcription Factor 1 (RUNX1) WB; IHC; ICC; IF.
Assay Kits CLT035Mu01 CLIA Kit for Runt Related Transcription Factor 1 (RUNX1) CLIA assay for Antigen Detection
ELT035Mu01 ELISA Kit for Runt Related Transcription Factor 1 (RUNX1) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Rattus norvegicus (Rat)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPT035Ra01 Recombinant Runt Related Transcription Factor 1 (RUNX1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAT035Ra01 Monoclonal Antibody to Runt Related Transcription Factor 1 (RUNX1) WB; IHC; ICC; IP.
PAT035Ra01 Polyclonal Antibody to Runt Related Transcription Factor 1 (RUNX1) WB; IHC; ICC; IF.
Assay Kits CLT035Ra01 CLIA Kit for Runt Related Transcription Factor 1 (RUNX1) CLIA assay for Antigen Detection
ELT035Ra01 ELISA Kit for Runt Related Transcription Factor 1 (RUNX1) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Canis familiaris; Canine (Dog)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPT035Ca01 Recombinant Runt Related Transcription Factor 1 (RUNX1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAT035Ca01 Monoclonal Antibody to Runt Related Transcription Factor 1 (RUNX1) WB; IHC; ICC; IP.
PAT035Ca01 Polyclonal Antibody to Runt Related Transcription Factor 1 (RUNX1) WB; IHC; ICC; IF.
Assay Kits CLT035Ca01 CLIA Kit for Runt Related Transcription Factor 1 (RUNX1) CLIA assay for Antigen Detection
ELT035Ca01 ELISA Kit for Runt Related Transcription Factor 1 (RUNX1) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Sus scrofa; Porcine (Pig)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPT035Po01 Recombinant Runt Related Transcription Factor 1 (RUNX1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAT035Po01 Monoclonal Antibody to Runt Related Transcription Factor 1 (RUNX1) WB; IHC; ICC; IP.
PAT035Po01 Polyclonal Antibody to Runt Related Transcription Factor 1 (RUNX1) WB; IHC; ICC; IF.
Assay Kits CLT035Po01 CLIA Kit for Runt Related Transcription Factor 1 (RUNX1) CLIA assay for Antigen Detection
ELT035Po01 ELISA Kit for Runt Related Transcription Factor 1 (RUNX1) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Bos taurus; Bovine (Cattle)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPT035Bo01 Recombinant Runt Related Transcription Factor 1 (RUNX1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAT035Bo01 Monoclonal Antibody to Runt Related Transcription Factor 1 (RUNX1) WB; IHC; ICC; IP.
PAT035Bo01 Polyclonal Antibody to Runt Related Transcription Factor 1 (RUNX1) WB; IHC; ICC; IF.
Assay Kits CLT035Bo01 CLIA Kit for Runt Related Transcription Factor 1 (RUNX1) CLIA assay for Antigen Detection
ELT035Bo01 ELISA Kit for Runt Related Transcription Factor 1 (RUNX1) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Oryctolagus cuniculus (Rabbit)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPT035Rb01 Recombinant Runt Related Transcription Factor 1 (RUNX1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAT035Rb01 Monoclonal Antibody to Runt Related Transcription Factor 1 (RUNX1) WB; IHC; ICC; IP.
PAT035Rb01 Polyclonal Antibody to Runt Related Transcription Factor 1 (RUNX1) WB; IHC; ICC; IF.
Assay Kits CLT035Rb01 CLIA Kit for Runt Related Transcription Factor 1 (RUNX1) CLIA assay for Antigen Detection
ELT035Rb01 ELISA Kit for Runt Related Transcription Factor 1 (RUNX1) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Rhesus monkey (Simian)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPT035Mo01 Recombinant Runt Related Transcription Factor 1 (RUNX1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAT035Mo01 Monoclonal Antibody to Runt Related Transcription Factor 1 (RUNX1) WB; IHC; ICC; IP.
PAT035Mo01 Polyclonal Antibody to Runt Related Transcription Factor 1 (RUNX1) WB; IHC; ICC; IF.
Assay Kits CLT035Mo01 CLIA Kit for Runt Related Transcription Factor 1 (RUNX1) CLIA assay for Antigen Detection
ELT035Mo01 ELISA Kit for Runt Related Transcription Factor 1 (RUNX1) Enzyme-linked immunosorbent assay for Antigen Detection

Reference

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"MOZ and MORF histone acetyltransferases interact with the Runt-domain transcription factor Runx2."
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"SUV39H1 interacts with AML1 and abrogates AML1 transactivity. AML1 is methylated in vivo."
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"Isoforms of the Ets transcription factor NERF/ELF-2 physically interact with AML1 and mediate opposing effects on AML1-mediated transcription of the B cell-specific blk gene."
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"RUNX1 associates with histone deacetylases and SUV39H1 to repress transcription."
Oncogene 25:5777-5786(2006) [PubMed] [Europe PMC] [Abstract]

"Cdk6 blocks myeloid differentiation by interfering with Runx1 DNA binding and Runx1-C/EBPalpha interaction."
EMBO J. 26:2361-2370(2007) [PubMed] [Europe PMC] [Abstract]

"LRP16 is fused to RUNX1 in monocytic leukemia cell line with t(11;21)(q13;q22)."
Eur. J. Haematol. 79:25-31(2007) [PubMed] [Europe PMC] [Abstract]

"Foxp3 controls regulatory T-cell function by interacting with AML1/Runx1."
Nature 446:685-689(2007) [PubMed] [Europe PMC] [Abstract]

"PEBP2-beta/CBF-beta-dependent phosphorylation of RUNX1 and p300 by HIPK2: implications for leukemogenesis."
Blood 112:3777-3787(2008) [PubMed] [Europe PMC] [Abstract]

"Yap1 phosphorylation by c-Abl is a critical step in selective activation of proapoptotic genes in response to DNA damage."
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"The leukemia-associated AML1 (Runx1) -- CBF beta complex functions as a DNA-induced molecular clamp."
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"DNA recognition by the RUNX1 transcription factor is mediated by an allosteric transition in the RUNT domain and by DNA bending."
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"Immunoglobulin motif DNA recognition and heterodimerization of the PEBP2/CBF Runt domain."
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"The Ig fold of the core binding factor alpha Runt domain is a member of a family of structurally and functionally related Ig-fold DNA-binding domains."
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"The RUNX1 Runt domain at 1.25A resolution: a structural switch and specifically bound chloride ions modulate DNA binding."
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Blood 86:1-14(1995) [PubMed] [Europe PMC] [Abstract]

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"Junctions of the AML1/MTG8(ETO) fusion are constant in t(8;21) acute myeloid leukemia detected by reverse transcription polymerase chain reaction."
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"The 3;21 translocation in myelodysplasia results in a fusion transcript between the AML1 gene and the gene for EAP, a highly conserved protein associated with the Epstein-Barr virus small RNA EBER 1."
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"Alternative, out-of-frame runt/MTG8 transcripts are encoded by the derivative (8) chromosome in the t(8;21) of acute myeloid leukemia M2."
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"The t(12;21) of acute lymphoblastic leukemia results in a tel-AML1 gene fusion."
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"Identification of two transcripts of AML1/ETO-fused gene in t(8;21) leukemic cells and expression of wild-type ETO gene in hematopoietic cells."
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"Fusion of the TEL gene on 12p13 to the AML1 gene on 21q22 in acute lymphoblastic leukemia."
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"Genome profiling of chronic myelomonocytic leukemia: frequent alterations of RAS and RUNX1 genes."
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