Phospholipid Scramblase 3 (PLSCR3)

Ca(2+)-dependent phospholipid scramblase 3

SPN	S923
HGNC	16495
MGI	1917560
PubMed	17355638
RGD	1307016
UniProt	Q9NRY6
Wiki	PLSCR3

Using the sequence of PLSCR1 as query, Wiedmer et al. (2000) identified an EST containing PLSCR3, and they cloned the full-length cDNA by PCR of an erythroleukemia cell cDNA library. The deduced 295-amino acid protein contains an N-terminal proline-rich region and a C-terminal Ca(2+)-binding domain. The proline-rich region contains several PxxP motifs that mediate binding to proteins containing SH3 and WW domains.

PLSCR3 shares 47% identity with PLSCR1, with most of the identity in the C-terminal region. Northern blot analysis revealed a 1.8-kb transcript expressed in most tissues and a 2.1-kb transcript expressed in skeletal muscle. Expression was below the limit of detection in testis, brain, and liver.

Reference

"Identification of three new members of the phospholipid scramblase gene family."
Biochim. Biophys. Acta 1467:244-253(2000) [PubMed] [Europe PMC] [Abstract]

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Phosphorylation of mitochondrial phospholipid scramblase 3 by protein kinase C-delta induces its activation and facilitates mitochondrial targeting of tBid."
J. Cell. Biochem. 101:1210-1221(2007) [PubMed] [Europe PMC] [Abstract]

"Identification of Alix-type and non-Alix-type ALG-2-binding sites in human phospholipid scramblase 3: differential binding to an alternatively spliced isoform and amino acid-substituted mutants."
J. Biol. Chem. 283:9623-9632(2008) [PubMed] [Europe PMC] [Abstract]