General Transcription Factor IIA, Polypeptide 2 (GTF2A2)

TFIIA; Transcription initiation factor IIA gamma chain

SPN	S892
HGNC	4647
MGI	1933289
RGD	620720
UniProt	P52657

Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A) and the general initiation factors TFIIA, TFIIB, TFIID, TFIIE, TFIIF, TFIIG/TFIIJ, and TFIIH. The first step involves recognition of the TATA element by the TATA-binding subunit (TBP) and may be regulated by TFIIA, a factor that interacts with both TBP and a TBP-associated factor (TAF) in TFIID.

TFIIA has 2 subunits (43 and 12 kD) in yeast and 3 subunits in higher eukaryotes. In HeLa extracts, it consists of a 35-kD alpha subunit and a 19-kD beta subunit encoded by the N- and C-terminal regions of GTF2A1 , respectively, and a 12-kD gamma subunit encoded by GTF2A2 .

Reference

"Reconstitution of human TFIIA activity from recombinant polypeptides: a role in TFIID-mediated transcription."
Genes Dev. 8:2336-2348(1994) [PubMed] [Europe PMC] [Abstract]

"Molecular cloning of the small (gamma) subunit of human TFIIA reveals functions critical for activated transcription."
Genes Dev. 8:2324-2335(1994) [PubMed] [Europe PMC] [Abstract]

"Human general transcription factor TFIIA: characterization of a cDNA encoding the small subunit and requirement for basal and activated transcription."
Proc. Natl. Acad. Sci. U.S.A. 92:3313-3317(1995) [PubMed] [Europe PMC] [Abstract]

"Analysis of the DNA sequence and duplication history of human chromosome 15."
Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Simian virus 40 large T antigen stabilizes the TATA-binding protein-TFIIA complex on the TATA element."
Mol. Cell. Biol. 18:3926-3935(1998) [PubMed] [Europe PMC] [Abstract]

"A nuclear factor ASC-2, as a cancer-amplified transcriptional coactivator essential for ligand-dependent transactivation by nuclear receptors in vivo."
J. Biol. Chem. 274:34283-34293(1999) [PubMed] [Europe PMC] [Abstract]

"TAC, a TBP-sans-TAFs complex containing the unprocessed TFIIAalphabeta precursor and the TFIIAgamma subunit."
Mol. Cell 6:527-537(2000) [PubMed] [Europe PMC] [Abstract]