Ubiquitin Conjugating Enzyme E2I (UBE2I)

P18; UBC9; UBCE9; SUMO-protein ligase; Ubiquitin carrier protein 9; Ubiquitin carrier protein I; Ubiquitin-protein ligase I; SUMO-conjugating enzyme UBC9

SPN	S201
HGNC	12485
MGI	107365
PubMed	12200128
RGD	3926
UniProt	P63279
Wiki	UBE2I

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s.

UBE2I encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene.The protein has a predicted sequence of 158 amino acids and can support the growth of yeast ubc9 temperature-sensitive mutants at non-permissive temperatures, indicating that their gene is a functional homolog of yeast ubc9.

Reference

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Nucleic Acids Res. 24:2005-2010(1996) [PubMed] [Europe PMC] [Abstract]

"Assignment of the gene for a ubiquitin-conjugating enzyme (UBE2I) to human chromosome band 16p13.3 by in situ hybridization."
Cytogenet. Cell Genet. 75:222-223(1996) [PubMed] [Europe PMC] [Abstract]

"Cloning, expression, and mapping of UBE2I, a novel gene encoding a human homologue of yeast ubiquitin-conjugating enzymes which are critical for regulating the cell cycle."
Cytogenet. Cell Genet. 72:86-89(1996) [PubMed] [Europe PMC] [Abstract]

"Poly(ADP-ribose) polymerase interacts with a novel human ubiquitin conjugating enzyme: hUbc9."
Gene 190:287-296(1997) [PubMed] [Europe PMC] [Abstract]

"Mammalian ubiquitin-conjugating enzyme Ubc9 interacts with Rad51 recombination protein and localizes in synaptonemal complexes."
Proc. Natl. Acad. Sci. U.S.A. 93:2958-2963(1996) [PubMed] [Europe PMC] [Abstract]

"Two-hybrid interaction of a human UBC9 homolog with centromere proteins of Saccharomyces cerevisiae."
Mol. Gen. Genet. 251:153-160(1996) [PubMed] [Europe PMC] [Abstract]

"Molecular cloning of the cDNA and chromosome localization of the gene for human ubiquitin-conjugating enzyme 9."
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Oncogene 15:1489-1495(1997) [PubMed] [Europe PMC] [Abstract]

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Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"mUBC9, a novel adenovirus E1A-interacting protein that complements a yeast cell cycle defect."
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"Mammalian homologs of seven in absentia regulate DCC via the ubiquitin-proteasome pathway."
Genes Dev. 11:2701-2714(1997) [PubMed] [Europe PMC] [Abstract]

"Ubc9 interacts with the androgen receptor and activates receptor-dependent transcription."
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"Association of FHIT (fragile histidine triad), a candidate tumour suppressor gene, with the ubiquitin-conjugating enzyme hUBC9."
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"Polymeric chains of SUMO-2 and SUMO-3 are conjugated to protein substrates by SAE1/SAE2 and Ubc9."
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"Transcription factor AP-2 interacts with the SUMO-conjugating enzyme UBC9 and is sumolated in vivo."
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"Role of two residues proximal to the active site of Ubc9 in substrate recognition by the Ubc9.SUMO-1 thiolester complex."
Biochemistry 42:3168-3179(2003) [PubMed] [Europe PMC] [Abstract]

"Role of an N-terminal site of Ubc9 in SUMO-1, -2, and -3 binding and conjugation."
Biochemistry 42:9959-9969(2003) [PubMed] [Europe PMC] [Abstract]

"The RanBP2 SUMO E3 ligase is neither HECT- nor RING-type."
Nat. Struct. Mol. Biol. 11:984-991(2004) [PubMed] [Europe PMC] [Abstract]

"Requirement of the coiled-coil domain of PML-RARalpha oncoprotein for localization, sumoylation, and inhibition of monocyte differentiation."
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"Unique binding interactions among Ubc9, SUMO and RanBP2 reveal a mechanism for SUMO paralog selection."
Nat. Struct. Mol. Biol. 12:67-74(2005) [PubMed] [Europe PMC] [Abstract]

"A general approach for investigating enzymatic pathways and substrates for ubiquitin-like modifiers."
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"Ubc9 interacts with SOX4 and represses its transcriptional activity."
Biochem. Biophys. Res. Commun. 344:727-734(2006) [PubMed] [Europe PMC] [Abstract]

"Functional interaction between human herpesvirus 6 immediate-early 2 protein and ubiquitin-conjugating enzyme 9 in the absence of sumoylation."
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"RSUME, a small RWD-containing protein, enhances SUMO conjugation and stabilizes HIF-1alpha during hypoxia."
Cell 131:309-323(2007) [PubMed] [Europe PMC] [Abstract]

"Functional characterization of TIP60 sumoylation in UV-irradiated DNA damage response."
Oncogene 27:931-941(2008) [PubMed] [Europe PMC] [Abstract]

"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]

"SUMOylation enhances DNA methyltransferase 1 activity."
Biochem. J. 421:449-461(2009) [PubMed] [Europe PMC] [Abstract]

"Sumoylation of forkhead L2 by Ubc9 is required for its activity as a transcriptional repressor of the steroidogenic acute regulatory gene."
Cell. Signal. 21:1935-1944(2009) [PubMed] [Europe PMC] [Abstract]

"SUMOylation of the mitochondrial fission protein Drp1 occurs at multiple nonconsensus sites within the B domain and is linked to its activity cycle."
FASEB J. 23:3917-3927(2009) [PubMed] [Europe PMC] [Abstract]

"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]

"Identification of a molecular recognition feature in the E1A oncoprotein that binds the SUMO conjugase UBC9 and likely interferes with polySUMOylation."
Oncogene 29:4693-4704(2010) [PubMed] [Europe PMC] [Abstract]

"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

"SUMOylation and SUMO-interacting motif (SIM) of metastasis tumor antigen 1 (MTA1) synergistically regulate its transcriptional repressor function."
J. Biol. Chem. 286:43793-43808(2011) [PubMed] [Europe PMC] [Abstract]

"Epstein-Barr virus latent membrane protein 1 (LMP1) C-terminal-activating region 3 contributes to LMP1-mediated cellular migration via its interaction with Ubc9."
J. Virol. 85:10144-10153(2011) [PubMed] [Europe PMC] [Abstract]

"The RAX/PACT-PKR stress response pathway promotes p53 sumoylation and activation, leading to G(1) arrest."
Cell Cycle 11:407-417(2012) [PubMed] [Europe PMC] [Abstract]

"Phosphorylation of Ubc9 by Cdk1 enhances SUMOylation activity."
PLoS ONE 7:E34250-E34250(2012) [PubMed] [Europe PMC] [Abstract]

"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]

"A SUMO-dependent interaction between Senataxin and the exosome, disrupted in the neurodegenerative disease AOA2, targets the exosome to sites of transcription-induced DNA damage."
Genes Dev. 27:2227-2232(2013) [PubMed] [Europe PMC] [Abstract]

"UHRF2, a ubiquitin E3 ligase, acts as a small ubiquitin-like modifier E3 ligase for zinc finger protein 131."
J. Biol. Chem. 288:9102-9111(2013) [PubMed] [Europe PMC] [Abstract]

"RSUME enhances glucocorticoid receptor SUMOylation and transcriptional activity."
Mol. Cell. Biol. 33:2116-2127(2013) [PubMed] [Europe PMC] [Abstract]

"In silico structural and functional characterization of the RSUME splice variants."
PLoS ONE 8:E57795-E57795(2013) [PubMed] [Europe PMC] [Abstract]

"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]

"Crystal structure of murine/human Ubc9 provides insight into the variability of the ubiquitin-conjugating system."
J. Biol. Chem. 272:21381-21387(1997) [PubMed] [Europe PMC] [Abstract]

"Structural basis for E2-mediated SUMO conjugation revealed by a complex between ubiquitin-conjugating enzyme Ubc9 and RanGAP1."
Cell 108:345-356(2002) [PubMed] [Europe PMC] [Abstract]

"Insights into E3 ligase activity revealed by a SUMO-RanGAP1-Ubc9-Nup358 complex."
Nature 435:687-692(2005) [PubMed] [Europe PMC] [Abstract]

"Lysine activation and functional analysis of E2-mediated conjugation in the SUMO pathway."
Nat. Struct. Mol. Biol. 13:491-499(2006) [PubMed] [Europe PMC] [Abstract]

"Structure and analysis of a complex between SUMO and Ubc9 illustrates features of a conserved E2-Ubl interaction."
J. Mol. Biol. 369:608-618(2007) [PubMed] [Europe PMC] [Abstract]

"Structural basis for regulation of poly-SUMO chain by a SUMO-like domain of Nip45."
Proteins 78:1491-1502(2010) [PubMed] [Europe PMC] [Abstract]

"Structure of Importin13-Ubc9 complex: nuclear import and release of a key regulator of sumoylation."
EMBO J. 30:427-438(2011) [PubMed] [Europe PMC] [Abstract]