Splicing Factor 1 (SF1)

ZFM1; ZNF162; BBP; Zinc Finger Protein 162; mBBP; Mammalian branch point-binding protein; Zinc finger gene in MEN1 locus; Transcription factor ZFM1

SPN	R691
HGNC	12950
MGI	1095403
PubMed	21146534
UniProt	Q15637
Wiki	SF1

The predicted 623-amino acid protein contains a nuclear transport domain, a metal-binding or zinc finger motif, and glutamine- and proline-rich regions. It shows some sequence similarity to WT1 and EGR2. RT-PCR was used to show expression in the thyroid gland, pancreas, adrenal gland, and ovary.

All of the ZNF162 isoforms contain a KH domain, a sequence motif present in proteins playing a major role in regulating cellular RNA metabolism. The gene contains 14 exons and spans approximately 15 kb. Analysis of the ZNF162 genomic cDNA sequences predicted the use of duplicated 5-prime and 3-prime splice sites as well as exon skipping and intron inclusion to generate 6 ZNF162 mRNAs by alternative splicing events.

Reference

"Mammalian splicing factor SF1 is encoded by variant cDNAs and binds to RNA."
RNA 2:794-810(1996) [PubMed] [Europe PMC] [Abstract]

"Identification of two novel isoforms of the ZNF162 gene: a growing family of signal transduction and activator of RNA proteins."
Genomics 42:268-277(1997) [PubMed] [Europe PMC] [Abstract]

"Isolation and characterization of a novel gene encoding nuclear protein at a locus (D11S636) tightly linked to multiple endocrine neoplasia type 1 (MEN1)."
Hum. Mol. Genet. 3:465-470(1994) [PubMed] [Europe PMC] [Abstract]

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

"Human chromosome 11 DNA sequence and analysis including novel gene identification."
Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Diverse modes of alternative splicing of human splicing factor SF1 deduced from the exon-intron structure of the gene."
Gene 211:29-37(1998) [PubMed] [Europe PMC] [Abstract]

, Submitted (MAY-2006) to UniProtKB

"Phosphorylation of splicing factor SF1 on Ser20 by cGMP-dependent protein kinase regulates spliceosome assembly."
EMBO J. 18:4549-4559(1999) [PubMed] [Europe PMC] [Abstract]

"Large-scale proteomic analysis of the human spliceosome."
Genome Res. 12:1231-1245(2002) [PubMed] [Europe PMC] [Abstract]

"The transcriptional repressor ZFM1 interacts with and modulates the ability of EWS to activate transcription."
J. Biol. Chem. 273:18086-18091(1998) [PubMed] [Europe PMC] [Abstract]

"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]

"A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]

"U2AF-homology motif interactions are required for alternative splicing regulation by SPF45."
Nat. Struct. Mol. Biol. 14:620-629(2007) [PubMed] [Europe PMC] [Abstract]

"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]

"A quantitative atlas of mitotic phosphorylation."
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]

"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]

"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]

"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]

"Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]

"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]

"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]

"Structural basis for recognition of the intron branch site RNA by splicing factor 1."
Science 294:1098-1102(2001) [PubMed] [Europe PMC] [Abstract]

"Structural basis for the molecular recognition between human splicing factors U2AF65 and SF1/mBBP."
Mol. Cell 11:965-976(2003) [PubMed] [Europe PMC] [Abstract]