Splicing Factor 3B Subunit 1 (SF3B1)

PRP10; PRPF10; SAP155; SF3b155; Spliceosome-associated protein 155; Pre-mRNA-splicing factor SF3b 155 kDa subunit

SPN	R689
HGNC	10768
MGI	1932339
PubMed	21998214
RGD	620148
UniProt	O75533
Wiki	SF3B1

Splicing factor 3B subunit 1 is subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP).

The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms.

Reference

"Phosphorylation of spliceosomal protein SAP 155 coupled with splicing catalysis."
Genes Dev. 12:1409-1414(1998) [PubMed] [Europe PMC] [Abstract]

"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]

"Functional association of U2 snRNP with the ATP-independent spliceosomal complex E."
Mol. Cell 5:779-787(2000) [PubMed] [Europe PMC] [Abstract]

"Characterization of novel SF3b and 17S U2 snRNP proteins, including a human Prp5p homologue and an SF3b DEAD-box protein."
EMBO J. 21:4978-4988(2002) [PubMed] [Europe PMC] [Abstract]

"Phosphorylation-dependent interaction between the splicing factors SAP155 and NIPP1."
J. Biol. Chem. 277:31834-31841(2002) [PubMed] [Europe PMC] [Abstract]

"Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis."
RNA 8:426-439(2002) [PubMed] [Europe PMC] [Abstract]

"Molecular architecture of the multiprotein splicing factor SF3b."
Science 300:980-984(2003) [PubMed] [Europe PMC] [Abstract]

"The human 18S U11/U12 snRNP contains a set of novel proteins not found in the U2-dependent spliceosome."
RNA 10:929-941(2004) [PubMed] [Europe PMC] [Abstract]

"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]

"The WSTF-SNF2h chromatin remodeling complex interacts with several nuclear proteins in transcription."
J. Biol. Chem. 281:16264-16271(2006) [PubMed] [Europe PMC] [Abstract]

"A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]

"Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra."
J. Proteome Res. 6:4150-4162(2007) [PubMed] [Europe PMC] [Abstract]

"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]

"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]

"A quantitative atlas of mitotic phosphorylation."
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]

"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]

"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]

"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]

"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

"Human senataxin resolves RNA/DNA hybrids formed at transcriptional pause sites to promote Xrn2-dependent termination."
Mol. Cell 42:794-805(2011) [PubMed] [Europe PMC] [Abstract]

"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]

"PQBP1, a factor linked to intellectual disability, affects alternative splicing associated with neurite outgrowth."
Genes Dev. 27:615-626(2013) [PubMed] [Europe PMC] [Abstract]

"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]

"Crystal structure of a core spliceosomal protein interface."
Proc. Natl. Acad. Sci. U.S.A. 103:1266-1271(2006) [PubMed] [Europe PMC] [Abstract]

"U2AF-homology motif interactions are required for alternative splicing regulation by SPF45."
Nat. Struct. Mol. Biol. 14:620-629(2007) [PubMed] [Europe PMC] [Abstract]

"NMR solution structure of the human spliceosomal protein complex p14-SF3B155."
Submitted (JAN-2007) to the PDB data bank

"Structural model of the p14/SF3b155 - branch duplex complex."
RNA 17:155-165(2011) [PubMed] [Europe PMC] [Abstract]

"Cancer-relevant splicing factor CAPERalpha engages the essential splicing factor SF3b155 in a specific ternary complex."
J. Biol. Chem. 289:17325-17337(2014) [PubMed] [Europe PMC] [Abstract]