Polymerase DNA Directed Beta (POLb)

SPN	R113
HGNC	9174
MGI	97740
PubMed	2261080
RGD	3363
UniProt	P06746
Wiki	POLB

DNA polymerase beta maintains genome integrity by participating in base excision repair. Over expression of POLB mRNA has been correlated with a number of cancer types whereas deficiencies in POLB results in hypersensitivity to alkylating agents, induced apoptosis and chromosomal breaking therefore it is essential that POLB expression is tightly regulated.

POLB gene is upregulated by CREB1 transcription factor binding to the cAMP response element(CRE) present in the promoter of the POLB gene in response to exposure to alkylating agents. POLB gene expression is also regulated at the post transcriptional level as the 3’UTR of the POLB mRNA has been shown to contain three stem-loop structures that influence gene expression.

Reference

"Molecular cloning and high-level expression of human polymerase beta cDNA and comparison of the purified recombinant human and rat enzymes."
Protein Expr. Purif. 18:100-110(2000) [PubMed] [Europe PMC] [Abstract]

"Polymorphisms in the human DNA polymerase beta gene."
Hum. Genet. 95:389-390(1995) [PubMed] [Europe PMC] [Abstract]

"The human DNA polymerase beta gene structure. Evidence of alternative splicing in gene expression."
Nucleic Acids Res. 22:2719-2725(1994) [PubMed] [Europe PMC] [Abstract]

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Human beta-polymerase gene. Structure of the 5'-flanking region and active promoter."
J. Biol. Chem. 263:16992-16998(1988) [PubMed] [Europe PMC] [Abstract]

"Expression of human DNA polymerase beta in Escherichia coli and characterization of the recombinant enzyme."
Biochemistry 27:901-909(1988) [PubMed] [Europe PMC] [Abstract]

"Sequence of human DNA polymerase beta mRNA obtained through cDNA cloning."
Biochem. Biophys. Res. Commun. 136:341-347(1986) [PubMed] [Europe PMC] [Abstract]

"Interaction of human apurinic endonuclease and DNA polymerase beta in the base excision repair pathway."
Proc. Natl. Acad. Sci. U.S.A. 94:7166-7169(1997) [PubMed] [Europe PMC] [Abstract]

"Catalytic center of DNA polymerase beta for excision of deoxyribose phosphate groups."
Biochemistry 37:6456-6464(1998) [PubMed] [Europe PMC] [Abstract]

"Covalent trapping of human DNA polymerase beta by the oxidative DNA lesion 2-deoxyribonolactone."
J. Biol. Chem. 277:7637-7640(2002) [PubMed] [Europe PMC] [Abstract]

"Arginine methylation regulates DNA polymerase beta."
Mol. Cell 22:51-62(2006) [PubMed] [Europe PMC] [Abstract]

"Ubiquitin ligase ARF-BP1/Mule modulates base excision repair."
EMBO J. 28:3207-3215(2009) [PubMed] [Europe PMC] [Abstract]

"USP47 is a deubiquitylating enzyme that regulates base excision repair by controlling steady-state levels of DNA Polymerase beta."
Mol. Cell 41:609-615(2011) [PubMed] [Europe PMC] [Abstract]

"A structural basis for metal ion mutagenicity and nucleotide selectivity in human DNA polymerase beta."
Biochemistry 35:12762-12777(1996) [PubMed] [Europe PMC] [Abstract]

"Characterization of the metal ion binding helix-hairpin-helix motifs in human DNA polymerase beta by X-ray structural analysis."
Biochemistry 35:12778-12787(1996) [PubMed] [Europe PMC] [Abstract]

"Crystal structures of human DNA polymerase beta complexed with gapped and nicked DNA: evidence for an induced fit mechanism."
Biochemistry 36:11205-11215(1997) [PubMed] [Europe PMC] [Abstract]

"Structure of DNA polymerase beta with the mutagenic DNA lesion 8-oxodeoxyguanine reveals structural insights into its coding potential."
Structure 11:121-127(2003) [PubMed] [Europe PMC] [Abstract]