Dedicator Of Cytokinesis 1 (DOCK1)

DOCK180; Ced5; DOwnstream Of CrK; 180 kDa protein downstream of CRK

SPN	Q632
HGNC	2987
MGI	2429765
PubMed	23592719
RGD	1566072
UniProt	Q14185
Wiki	Dock180

Dock180 is part of a large class of proteins (GEFs) which contibrute to cellular signalling events by activating small G proteins. In their resting state G proteins are bound to Guanosine diphosphate (GDP) and their activation requires the dissociation of GDP and binding of guanosine triphosphate (GTP). GEFs activate G proteins by promoting this nucleotide exchange.Dock180 and related proteins differ from other GEFs in that they do not possess the canonical structure of tandem DH-PH domains known to elicit nucleotide exchange. Instead they possess a DHR2 domain which mediates Rac activation by stabilising it in its nucleotide-free state. Dock180 was identified, using a far-western blotting approach, as a binding partner of the adaptor protein Crk that was able to induce morphological changes in 3T3 fibroblasts.

Reference

"DOCK180, a major CRK-binding protein, alters cell morphology upon translocation to the cell membrane."
Mol. Cell. Biol. 16:1770-1776(1996) [PubMed] [Europe PMC] [Abstract]

"The DNA sequence and comparative analysis of human chromosome 10."
Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]

"Interaction between the amino-terminal SH3 domain of CRK and its natural target proteins."
J. Biol. Chem. 271:14468-14472(1996) [PubMed] [Europe PMC] [Abstract]

"Activation of Rac1 by a Crk SH3-binding protein, DOCK180."
Genes Dev. 12:3331-3336(1998) [PubMed] [Europe PMC] [Abstract]

"Identification and kinetic analysis of the interaction between Nck-2 and DOCK180."
FEBS Lett. 491:193-199(2001) [PubMed] [Europe PMC] [Abstract]

"Membrane recruitment of DOCK180 by binding to PtdIns(3,4,5)P3."
Biochem. J. 354:73-78(2001) [PubMed] [Europe PMC] [Abstract]

"Unconventional Rac-GEF activity is mediated through the Dock180-ELMO complex."
Nat. Cell Biol. 4:574-582(2002) [PubMed] [Europe PMC] [Abstract]

"Identification of an evolutionarily conserved superfamily of DOCK180-related proteins with guanine nucleotide exchange activity."
J. Cell Sci. 115:4901-4913(2002) [PubMed] [Europe PMC] [Abstract]

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Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

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BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]