Endoplasmic Reticulum Aminopeptidase 2 (ERAP2)

L-RAP; LRAP; Leukocyte-Derived Arginine Aminopeptidase

SPN	Q353
HGNC	29499
PubMed	15908954
UniProt	Q6P179
Wiki	ERAP2

Aminopeptidases hydrolyze N-terminal amino acids of proteins or peptide substrates. Major histocompatibility complex (MHC) class I molecules rely on aminopeptidases such as ERAP1 and LRAP to trim precursors to antigenic peptides in the endoplasmic reticulum (ER) following cleavage in the cytoplasm by tripeptidyl peptidase II (TPP2) The deduced full-length protein contains 960 amino acids and has a calculated molecular mass of 123 kD. It shares 43% and 49% identity with PLAP and ERAP1, respectively, and contains a hydrophobic N-terminal region, zinc metallopeptidase (M1) motifs, and 9 potential N-glycosylation sites. The truncated form, LRAP(s), contains 532 amino acids. Northern blot analysis using the full-length LRAP cDNA as probe detected ubiquitous expression of a 6.7-kb transcript, with relatively high expression in spleen and leukocytes.

Reference

"Human leukocyte-derived arginine aminopeptidase. The third member of the oxytocinase subfamily of aminopeptidases."
J. Biol. Chem. 278:32275-32283(2003) [PubMed] [Europe PMC] [Abstract]

"Regulation of the human leukocyte-derived arginine aminopeptidase/endoplasmic reticulum-aminopeptidase 2 gene by interferon-gamma."
FEBS J. 272:916-928(2005) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry."
J. Proteome Res. 4:2070-2080(2005) [PubMed] [Europe PMC] [Abstract]

"Concerted peptide trimming by human ERAP1 and ERAP2 aminopeptidase complexes in the endoplasmic reticulum."
Nat. Immunol. 6:689-697(2005) [PubMed] [Europe PMC] [Abstract]

"The ER aminopeptidase, ERAP1, trims precursors to lengths of MHC class I peptides by a 'molecular ruler' mechanism."
Proc. Natl. Acad. Sci. U.S.A. 102:17107-17112(2005) [PubMed] [Europe PMC] [Abstract]

"Expression of endoplasmic reticulum aminopeptidases in EBV-B cell lines from healthy donors and in leukemia/lymphoma, carcinoma, and melanoma cell lines."
J. Immunol. 176:4869-4879(2006) [PubMed] [Europe PMC] [Abstract]

"Inactivation of RB1 in mantle-cell lymphoma detected by nonsense-mediated mRNA decay pathway inhibition and microarray analysis."
Blood 109:5422-5429(2007) [PubMed] [Europe PMC] [Abstract]

"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

"The crystal structure of human endoplasmic reticulum aminopeptidase 2 reveals the atomic basis for distinct roles in antigen processing."
Biochemistry 51:286-295(2012) [PubMed] [Europe PMC] [Abstract]