Phosphoprotein Associated With Glycosphingolipid Microdomains 1 (PAG1)
CBP; PAG; Csk-Binding Protein; Transmembrane adapter protein PAG; Transmembrane phosphoprotein Cbp
PAG1 is a type III transmembrane adaptor protein that binds to the tyrosine kinase csk protein. It is thought to be involved in the regulation of T cell activation.Initiation of signal transduction via immunoreceptors depends on interactions of the engaged receptor with glycosphingolipid-enriched membrane microdomains (GEMs), also known as membrane rafts. GEMs are small signaling islets that are relatively resistant to detergent solubilization except by n-alkyl glycosides. Sequence analysis predicted that the 432-amino acid type III transmembrane protein contains a 16-amino acid extracellular domain, a 20-amino acid transmembrane domain, a putative palmitoylation site, and a 397-amino acid cytoplasmic tail with 9 potential tyrosine phosphorylation sites and multiple putative casein kinase II and protein kinase C phosphorylation sites.
Organism species: Homo sapiens (Human)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPQ255Hu01 | Recombinant Phosphoprotein Associated With Glycosphingolipid Microdomains 1 (PAG1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAQ255Hu01 | Monoclonal Antibody to Phosphoprotein Associated With Glycosphingolipid Microdomains 1 (PAG1) | WB; IHC; ICC; IP. |
| PAQ255Hu01 | Polyclonal Antibody to Phosphoprotein Associated With Glycosphingolipid Microdomains 1 (PAG1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLQ255Hu01 | CLIA Kit for Phosphoprotein Associated With Glycosphingolipid Microdomains 1 (PAG1) | CLIA assay for Antigen Detection |
| ELQ255Hu01 | ELISA Kit for Phosphoprotein Associated With Glycosphingolipid Microdomains 1 (PAG1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Mus musculus (Mouse)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPQ255Mu01 | Recombinant Phosphoprotein Associated With Glycosphingolipid Microdomains 1 (PAG1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAQ255Mu01 | Monoclonal Antibody to Phosphoprotein Associated With Glycosphingolipid Microdomains 1 (PAG1) | WB; IHC; ICC; IP. |
| PAQ255Mu01 | Polyclonal Antibody to Phosphoprotein Associated With Glycosphingolipid Microdomains 1 (PAG1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLQ255Mu01 | CLIA Kit for Phosphoprotein Associated With Glycosphingolipid Microdomains 1 (PAG1) | CLIA assay for Antigen Detection |
| ELQ255Mu01 | ELISA Kit for Phosphoprotein Associated With Glycosphingolipid Microdomains 1 (PAG1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Rattus norvegicus (Rat)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPQ255Ra01 | Recombinant Phosphoprotein Associated With Glycosphingolipid Microdomains 1 (PAG1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAQ255Ra01 | Monoclonal Antibody to Phosphoprotein Associated With Glycosphingolipid Microdomains 1 (PAG1) | WB; IHC; ICC; IP. |
| PAQ255Ra01 | Polyclonal Antibody to Phosphoprotein Associated With Glycosphingolipid Microdomains 1 (PAG1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLQ255Ra01 | CLIA Kit for Phosphoprotein Associated With Glycosphingolipid Microdomains 1 (PAG1) | CLIA assay for Antigen Detection |
| ELQ255Ra01 | ELISA Kit for Phosphoprotein Associated With Glycosphingolipid Microdomains 1 (PAG1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Canis familiaris; Canine (Dog)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPQ255Ca01 | Recombinant Phosphoprotein Associated With Glycosphingolipid Microdomains 1 (PAG1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAQ255Ca01 | Monoclonal Antibody to Phosphoprotein Associated With Glycosphingolipid Microdomains 1 (PAG1) | WB; IHC; ICC; IP. |
| PAQ255Ca01 | Polyclonal Antibody to Phosphoprotein Associated With Glycosphingolipid Microdomains 1 (PAG1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLQ255Ca01 | CLIA Kit for Phosphoprotein Associated With Glycosphingolipid Microdomains 1 (PAG1) | CLIA assay for Antigen Detection |
| ELQ255Ca01 | ELISA Kit for Phosphoprotein Associated With Glycosphingolipid Microdomains 1 (PAG1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Sus scrofa; Porcine (Pig)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPQ255Po01 | Recombinant Phosphoprotein Associated With Glycosphingolipid Microdomains 1 (PAG1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAQ255Po01 | Monoclonal Antibody to Phosphoprotein Associated With Glycosphingolipid Microdomains 1 (PAG1) | WB; IHC; ICC; IP. |
| PAQ255Po01 | Polyclonal Antibody to Phosphoprotein Associated With Glycosphingolipid Microdomains 1 (PAG1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLQ255Po01 | CLIA Kit for Phosphoprotein Associated With Glycosphingolipid Microdomains 1 (PAG1) | CLIA assay for Antigen Detection |
| ELQ255Po01 | ELISA Kit for Phosphoprotein Associated With Glycosphingolipid Microdomains 1 (PAG1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Bos taurus; Bovine (Cattle)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPQ255Bo01 | Recombinant Phosphoprotein Associated With Glycosphingolipid Microdomains 1 (PAG1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAQ255Bo01 | Monoclonal Antibody to Phosphoprotein Associated With Glycosphingolipid Microdomains 1 (PAG1) | WB; IHC; ICC; IP. |
| PAQ255Bo01 | Polyclonal Antibody to Phosphoprotein Associated With Glycosphingolipid Microdomains 1 (PAG1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLQ255Bo01 | CLIA Kit for Phosphoprotein Associated With Glycosphingolipid Microdomains 1 (PAG1) | CLIA assay for Antigen Detection |
| ELQ255Bo01 | ELISA Kit for Phosphoprotein Associated With Glycosphingolipid Microdomains 1 (PAG1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Oryctolagus cuniculus (Rabbit)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPQ255Rb01 | Recombinant Phosphoprotein Associated With Glycosphingolipid Microdomains 1 (PAG1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAQ255Rb01 | Monoclonal Antibody to Phosphoprotein Associated With Glycosphingolipid Microdomains 1 (PAG1) | WB; IHC; ICC; IP. |
| PAQ255Rb01 | Polyclonal Antibody to Phosphoprotein Associated With Glycosphingolipid Microdomains 1 (PAG1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLQ255Rb01 | CLIA Kit for Phosphoprotein Associated With Glycosphingolipid Microdomains 1 (PAG1) | CLIA assay for Antigen Detection |
| ELQ255Rb01 | ELISA Kit for Phosphoprotein Associated With Glycosphingolipid Microdomains 1 (PAG1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Rhesus monkey (Simian)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPQ255Mo01 | Recombinant Phosphoprotein Associated With Glycosphingolipid Microdomains 1 (PAG1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAQ255Mo01 | Monoclonal Antibody to Phosphoprotein Associated With Glycosphingolipid Microdomains 1 (PAG1) | WB; IHC; ICC; IP. |
| PAQ255Mo01 | Polyclonal Antibody to Phosphoprotein Associated With Glycosphingolipid Microdomains 1 (PAG1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLQ255Mo01 | CLIA Kit for Phosphoprotein Associated With Glycosphingolipid Microdomains 1 (PAG1) | CLIA assay for Antigen Detection |
| ELQ255Mo01 | ELISA Kit for Phosphoprotein Associated With Glycosphingolipid Microdomains 1 (PAG1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Reference
"Phosphoprotein associated with glycosphingolipid-enriched microdomains (PAG), a novel ubiquitously expressed transmembrane adaptor protein, binds the protein tyrosine kinase csk and is involved in regulation of T cell activation."
J. Exp. Med. 191:1591-1604(2000) [PubMed] [Europe PMC] [Abstract]
"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
"Interaction between two adapter proteins, PAG and EBP50: a possible link between membrane rafts and actin cytoskeleton."
FEBS Lett. 507:133-136(2001) [PubMed] [Europe PMC] [Abstract]
"Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry."
Anal. Chem. 76:2763-2772(2004) [PubMed] [Europe PMC] [Abstract]
"Transmembrane adaptor molecules: a new category of lymphoid-cell markers."
Blood 107:213-221(2006) [PubMed] [Europe PMC] [Abstract]
"Oncogenic association of the Cbp/PAG adaptor protein with the Lyn tyrosine kinase in human B-NHL rafts."
Blood 111:2310-2320(2008) [PubMed] [Europe PMC] [Abstract]
"Large-scale proteomics analysis of the human kinome."
Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]
"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]