Lens Epithelium Derived Growth Factor (LEDGF)

PSIP1; p75; p52; DFS70; PAIP; PSIP2; PC4 And SFRS1 Interacting Protein 1; CLL-associated antigen KW-7; Dense fine speckles 70 kDa protein; Transcriptional coactivator p75/p52

SPN	Q131
HGNC	9527
MGI	2142116
PubMed	22773103
RGD	631439
UniProt	O75475
Wiki	PSIP1

PSIP1 is a misnomer as the protein is present in most tissues and has no direct role in the development of Lens epithelium. LEDGF/p75, a transcription coactivator, gained prominence as the host factor which assists HIV integration and is probably the only protein whose knock down severely affects the HIV integration levels. The interaction between HIV integrase and human LEDGF/p75 is a promising target for anti-HIV drug discovery. Expressed at high level in the thymus. Expressed in fetal and adult brain. Expressed in neurons, but not astrocytes. Markedly elevated in fetal as compared to adult brain. In the adult brain, expressed in the subventricular zone (SVZ), in hippocampus, and undetectable elsewhere. In the fetal brain, expressed in the germinal neuroepithelium and cortical plate regions.

Reference

"Isolation of cDNAs encoding novel transcription coactivators p52 and p75 reveals an alternate regulatory mechanism of transcriptional activation."
EMBO J. 17:6723-6729(1998) [PubMed] [Europe PMC] [Abstract]

"Lens epithelium-derived growth factor: effects on growth and survival of lens epithelial cells, keratinocytes, and fibroblasts."
Biochem. Biophys. Res. Commun. 267:373-381(2000) [PubMed] [Europe PMC] [Abstract]

"Lens epithelium-derived growth factor (LEDGF/p75) and p52 are derived from a single gene by alternative splicing."
Gene 242:265-273(2000) [PubMed] [Europe PMC] [Abstract]

"Identification of tumor-associated antigens in chronic lymphocytic leukemia by SEREX."
Blood 100:2123-2131(2002) [PubMed] [Europe PMC] [Abstract]

"DNA sequence and analysis of human chromosome 9."
Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Autoantibodies to DFS 70 kd/transcription coactivator p75 in atopic dermatitis and other conditions."
J. Allergy Clin. Immunol. 105:1211-1220(2000) [PubMed] [Europe PMC] [Abstract]

"Lens epithelium-derived growth factor (LEDGF/p75) expression in fetal and adult human brain."
Exp. Eye Res. 79:941-948(2004) [PubMed] [Europe PMC] [Abstract]

"Lens epithelium-derived growth factor/p75 prevents proteasomal degradation of HIV-1 integrase."
J. Biol. Chem. 279:55570-55577(2004) [PubMed] [Europe PMC] [Abstract]

"The interaction of LEDGF/p75 with integrase is lentiviral-specific and promotes DNA binding."
J. Biol. Chem. 280:17841-17847(2005) [PubMed] [Europe PMC] [Abstract]

"Identification of the LEDGF/p75 HIV-1 integrase-interaction domain and NLS reveals NLS-independent chromatin tethering."
J. Cell Sci. 118:1733-1743(2005) [PubMed] [Europe PMC] [Abstract]

"A novel transcriptional coactivator, p52, functionally interacts with the essential splicing factor ASF/SF2."
Mol. Cell 2:751-759(1998) [PubMed] [Europe PMC] [Abstract]

"t(9;11)(p22;p15) with NUP98-LEDGF fusion gene in pediatric acute myeloid leukemia."
Leuk. Res. 29:467-470(2005) [PubMed] [Europe PMC] [Abstract]

"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]

"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."
Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]

"Phosphorylation analysis of primary human T lymphocytes using sequential IMAC and titanium oxide enrichment."
J. Proteome Res. 7:5167-5176(2008) [PubMed] [Europe PMC] [Abstract]

"A quantitative atlas of mitotic phosphorylation."
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

"Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography."
Proteomics 8:1346-1361(2008) [PubMed] [Europe PMC] [Abstract]

"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]

"Lens epithelium-derived growth factor/p75 interacts with the transposase-derived DDE domain of PogZ."
J. Biol. Chem. 284:11467-11477(2009) [PubMed] [Europe PMC] [Abstract]

"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]

"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]

"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]

"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]

"Solution structure of the HIV-1 integrase-binding domain in LEDGF/p75."
Nat. Struct. Mol. Biol. 12:526-532(2005) [PubMed] [Europe PMC] [Abstract]

"Structural basis for the recognition between HIV-1 integrase and transcriptional coactivator p75."
Proc. Natl. Acad. Sci. U.S.A. 102:17308-17313(2005) [PubMed] [Europe PMC] [Abstract]